Prey killing without invasion by Bdellovibrio bacteriovorus defective for a MIDAS-family adhesin
Abstract The bacterium Bdellovibrio bacteriovorus is a predator of other Gram-negative bacteria. The predator invades the prey’s periplasm and modifies the prey’s cell wall, forming a rounded killed prey, or bdelloplast, containing a live B. bacteriovorus. Redundancy in adhesive processes makes inva...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-47412-3 |
| _version_ | 1797209203750731776 |
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| author | Jess Tyson Paul Radford Carey Lambert Rob Till Simona G. Huwiler Andrew L. Lovering R. Elizabeth Sockett |
| author_facet | Jess Tyson Paul Radford Carey Lambert Rob Till Simona G. Huwiler Andrew L. Lovering R. Elizabeth Sockett |
| author_sort | Jess Tyson |
| collection | DOAJ |
| description | Abstract The bacterium Bdellovibrio bacteriovorus is a predator of other Gram-negative bacteria. The predator invades the prey’s periplasm and modifies the prey’s cell wall, forming a rounded killed prey, or bdelloplast, containing a live B. bacteriovorus. Redundancy in adhesive processes makes invasive mutants rare. Here, we identify a MIDAS adhesin family protein, Bd0875, that is expressed at the predator-prey invasive junction and is important for successful invasion of prey. A mutant strain lacking bd0875 is still able to form round, dead bdelloplasts; however, 10% of the bdelloplasts do not contain B. bacteriovorus, indicative of an invasion defect. Bd0875 activity requires the conserved MIDAS motif, which is linked to catch-and-release activity of MIDAS proteins in other organisms. A proteomic analysis shows that the uninvaded bdelloplasts contain B. bacteriovorus proteins, which are likely secreted into the prey by the Δbd0875 predator during an abortive invasion period. Thus, secretion of proteins into the prey seems to be sufficient for prey killing, even in the absence of a live predator inside the prey periplasm. |
| first_indexed | 2024-04-24T09:50:58Z |
| format | Article |
| id | doaj.art-4f503fa5a7a148cf87614ceee5ab0c4e |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-24T09:50:58Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-4f503fa5a7a148cf87614ceee5ab0c4e2024-04-14T11:22:34ZengNature PortfolioNature Communications2041-17232024-04-0115111110.1038/s41467-024-47412-3Prey killing without invasion by Bdellovibrio bacteriovorus defective for a MIDAS-family adhesinJess Tyson0Paul Radford1Carey Lambert2Rob Till3Simona G. Huwiler4Andrew L. Lovering5R. Elizabeth Sockett6School of Life Sciences, University of Nottingham, Medical School, Queen’s Medical CentreSchool of Life Sciences, University of Nottingham, Medical School, Queen’s Medical CentreSchool of Life Sciences, University of Nottingham, Medical School, Queen’s Medical CentreSchool of Life Sciences, University of Nottingham, Medical School, Queen’s Medical CentreDepartment of Plant & Microbial Biology, University of Zurich, CH-School of Biosciences, University of BirminghamSchool of Life Sciences, University of Nottingham, Medical School, Queen’s Medical CentreAbstract The bacterium Bdellovibrio bacteriovorus is a predator of other Gram-negative bacteria. The predator invades the prey’s periplasm and modifies the prey’s cell wall, forming a rounded killed prey, or bdelloplast, containing a live B. bacteriovorus. Redundancy in adhesive processes makes invasive mutants rare. Here, we identify a MIDAS adhesin family protein, Bd0875, that is expressed at the predator-prey invasive junction and is important for successful invasion of prey. A mutant strain lacking bd0875 is still able to form round, dead bdelloplasts; however, 10% of the bdelloplasts do not contain B. bacteriovorus, indicative of an invasion defect. Bd0875 activity requires the conserved MIDAS motif, which is linked to catch-and-release activity of MIDAS proteins in other organisms. A proteomic analysis shows that the uninvaded bdelloplasts contain B. bacteriovorus proteins, which are likely secreted into the prey by the Δbd0875 predator during an abortive invasion period. Thus, secretion of proteins into the prey seems to be sufficient for prey killing, even in the absence of a live predator inside the prey periplasm.https://doi.org/10.1038/s41467-024-47412-3 |
| spellingShingle | Jess Tyson Paul Radford Carey Lambert Rob Till Simona G. Huwiler Andrew L. Lovering R. Elizabeth Sockett Prey killing without invasion by Bdellovibrio bacteriovorus defective for a MIDAS-family adhesin Nature Communications |
| title | Prey killing without invasion by Bdellovibrio bacteriovorus defective for a MIDAS-family adhesin |
| title_full | Prey killing without invasion by Bdellovibrio bacteriovorus defective for a MIDAS-family adhesin |
| title_fullStr | Prey killing without invasion by Bdellovibrio bacteriovorus defective for a MIDAS-family adhesin |
| title_full_unstemmed | Prey killing without invasion by Bdellovibrio bacteriovorus defective for a MIDAS-family adhesin |
| title_short | Prey killing without invasion by Bdellovibrio bacteriovorus defective for a MIDAS-family adhesin |
| title_sort | prey killing without invasion by bdellovibrio bacteriovorus defective for a midas family adhesin |
| url | https://doi.org/10.1038/s41467-024-47412-3 |
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