Comparison of two methods for determining human adipose cell size
The mean cell sizes of specimens of human adipose tissue were determined on sectioned slices according to the method described by Sjöström et al. (J. Lipid Res. 1971. 12: 521–530) and on adipocytes isolated after treatment of the tissue with collagenase. The average mean cell sizes from 11 biopsy sp...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1972-11-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520393561 |
| _version_ | 1818743171837853696 |
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| author | Ulf Smith Lars Sjöström Per Björntorp |
| author_facet | Ulf Smith Lars Sjöström Per Björntorp |
| author_sort | Ulf Smith |
| collection | DOAJ |
| description | The mean cell sizes of specimens of human adipose tissue were determined on sectioned slices according to the method described by Sjöström et al. (J. Lipid Res. 1971. 12: 521–530) and on adipocytes isolated after treatment of the tissue with collagenase. The average mean cell sizes from 11 biopsy specimens were 94.4 and 94.0 μm, respectively (r = 0.964; P(tb) < 0.001; y = 0.90x + 9.74), for the two methods. There was no indication of an increased rupture of isolated large human adipose cells. Thus, with precautions (freshly siliconized glassware and omitting the centrifugation of the isolated cells), the collagenase method may be used for metabolic as well as morphologic studies of human adipose tissue. |
| first_indexed | 2024-12-18T02:24:11Z |
| format | Article |
| id | doaj.art-4f513caf5d584d32b36a9f4c63f967a8 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-18T02:24:11Z |
| publishDate | 1972-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-4f513caf5d584d32b36a9f4c63f967a82022-12-21T21:24:06ZengElsevierJournal of Lipid Research0022-22751972-11-01136822824Comparison of two methods for determining human adipose cell sizeUlf Smith0Lars Sjöström1Per Björntorp2Departments of Medicine I and II, University of Gothenburg, Sahlgren's Hospital, Gothenburg, SwedenDepartments of Medicine I and II, University of Gothenburg, Sahlgren's Hospital, Gothenburg, SwedenDepartments of Medicine I and II, University of Gothenburg, Sahlgren's Hospital, Gothenburg, SwedenThe mean cell sizes of specimens of human adipose tissue were determined on sectioned slices according to the method described by Sjöström et al. (J. Lipid Res. 1971. 12: 521–530) and on adipocytes isolated after treatment of the tissue with collagenase. The average mean cell sizes from 11 biopsy specimens were 94.4 and 94.0 μm, respectively (r = 0.964; P(tb) < 0.001; y = 0.90x + 9.74), for the two methods. There was no indication of an increased rupture of isolated large human adipose cells. Thus, with precautions (freshly siliconized glassware and omitting the centrifugation of the isolated cells), the collagenase method may be used for metabolic as well as morphologic studies of human adipose tissue.http://www.sciencedirect.com/science/article/pii/S0022227520393561collagenase |
| spellingShingle | Ulf Smith Lars Sjöström Per Björntorp Comparison of two methods for determining human adipose cell size Journal of Lipid Research collagenase |
| title | Comparison of two methods for determining human adipose cell size |
| title_full | Comparison of two methods for determining human adipose cell size |
| title_fullStr | Comparison of two methods for determining human adipose cell size |
| title_full_unstemmed | Comparison of two methods for determining human adipose cell size |
| title_short | Comparison of two methods for determining human adipose cell size |
| title_sort | comparison of two methods for determining human adipose cell size |
| topic | collagenase |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520393561 |
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