The Hydrolytic Peptides of Soybean Protein Induce Cell Cycle Arrest and Apoptosis on Human Oral Cancer Cell Line HSC-3
Protein hydrolysates from various sources, including tuna cooking juice, soy protein isolate, sodium caseinate, wheat gluten and skin gelatin from porcine, tilapia, halibut and milkfish were analyzed to screen their antiproliferative activities against the human oral squamous carcinoma cell line, HS...
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MDPI AG
2022-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/9/2839 |
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| author | Cheng-Hong Hsieh Tzu-Yuan Wang Bo-Chen Tung Hui-Ping Liu Lien-Te Yeh Kuo-Chiang Hsu |
| author_facet | Cheng-Hong Hsieh Tzu-Yuan Wang Bo-Chen Tung Hui-Ping Liu Lien-Te Yeh Kuo-Chiang Hsu |
| author_sort | Cheng-Hong Hsieh |
| collection | DOAJ |
| description | Protein hydrolysates from various sources, including tuna cooking juice, soy protein isolate, sodium caseinate, wheat gluten and skin gelatin from porcine, tilapia, halibut and milkfish were analyzed to screen their antiproliferative activities against the human oral squamous carcinoma cell line, HSC-3. The soy protein isolate was selected for further investigations based on its hydrolysates with bromelain (SB) and thermolysin (ST), showing the greatest inhibition of cell growth. The SB and ST hydrolysates showed antiproliferative activities up to 35.45–76.39% against HSC-3 cells at 72 h, and their IC<sub>50</sub> values were 0.74 and 0.60 mg/mL, respectively. SB and ST induced cell cycle arrest in the S phase through a pathway independent of p21 and p27 protein expression. Further, ST induced the apoptosis of HSC-3 cells by downregulating expression of Bcl-2, PARP, caspase 3 and caspase 9, but an upregulating expression of p53 and cleaved caspase 3. Unlike ST, SB may induce necrosis on HSC-3 cells. Thus, soybean hydrolysates may be a good source for providing antiproliferative peptides against HSC-3, while SB and ST may have the potential to be developed as functional foods. |
| first_indexed | 2024-03-10T03:53:33Z |
| format | Article |
| id | doaj.art-4f55c20dd8c64ea193723fc90171e762 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:53:33Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4f55c20dd8c64ea193723fc90171e7622023-11-23T08:50:24ZengMDPI AGMolecules1420-30492022-04-01279283910.3390/molecules27092839The Hydrolytic Peptides of Soybean Protein Induce Cell Cycle Arrest and Apoptosis on Human Oral Cancer Cell Line HSC-3Cheng-Hong Hsieh0Tzu-Yuan Wang1Bo-Chen Tung2Hui-Ping Liu3Lien-Te Yeh4Kuo-Chiang Hsu5Department of Food Nutrition and Health Biotechnology, Asia University, 500 Lioufeng Rd., Wufeng, Taichung City 41354, TaiwanDivision of Endocrine and Metabolism, China Medical University Hospital, 2 Yude Rd., Taichung City 40447, TaiwanDepartment of Nutrition, China Medical University, No. 100, Sec. 1, Jingmao Rd., Beitun Dist., Taichung City 40604, TaiwanDepartment of Nutrition, China Medical University, No. 100, Sec. 1, Jingmao Rd., Beitun Dist., Taichung City 40604, TaiwanDepartment of Baking Technology and Management, National Kaohsiung University of Hospitality and Tourism, No. 1, Songhe Rd., Xiaogang Dist., Kaohsiung City 81271, TaiwanDepartment of Food Nutrition and Health Biotechnology, Asia University, 500 Lioufeng Rd., Wufeng, Taichung City 41354, TaiwanProtein hydrolysates from various sources, including tuna cooking juice, soy protein isolate, sodium caseinate, wheat gluten and skin gelatin from porcine, tilapia, halibut and milkfish were analyzed to screen their antiproliferative activities against the human oral squamous carcinoma cell line, HSC-3. The soy protein isolate was selected for further investigations based on its hydrolysates with bromelain (SB) and thermolysin (ST), showing the greatest inhibition of cell growth. The SB and ST hydrolysates showed antiproliferative activities up to 35.45–76.39% against HSC-3 cells at 72 h, and their IC<sub>50</sub> values were 0.74 and 0.60 mg/mL, respectively. SB and ST induced cell cycle arrest in the S phase through a pathway independent of p21 and p27 protein expression. Further, ST induced the apoptosis of HSC-3 cells by downregulating expression of Bcl-2, PARP, caspase 3 and caspase 9, but an upregulating expression of p53 and cleaved caspase 3. Unlike ST, SB may induce necrosis on HSC-3 cells. Thus, soybean hydrolysates may be a good source for providing antiproliferative peptides against HSC-3, while SB and ST may have the potential to be developed as functional foods.https://www.mdpi.com/1420-3049/27/9/2839soy protein isolateanticancer peptidesoral cancerantiproliferationcell cycleapoptosis |
| spellingShingle | Cheng-Hong Hsieh Tzu-Yuan Wang Bo-Chen Tung Hui-Ping Liu Lien-Te Yeh Kuo-Chiang Hsu The Hydrolytic Peptides of Soybean Protein Induce Cell Cycle Arrest and Apoptosis on Human Oral Cancer Cell Line HSC-3 Molecules soy protein isolate anticancer peptides oral cancer antiproliferation cell cycle apoptosis |
| title | The Hydrolytic Peptides of Soybean Protein Induce Cell Cycle Arrest and Apoptosis on Human Oral Cancer Cell Line HSC-3 |
| title_full | The Hydrolytic Peptides of Soybean Protein Induce Cell Cycle Arrest and Apoptosis on Human Oral Cancer Cell Line HSC-3 |
| title_fullStr | The Hydrolytic Peptides of Soybean Protein Induce Cell Cycle Arrest and Apoptosis on Human Oral Cancer Cell Line HSC-3 |
| title_full_unstemmed | The Hydrolytic Peptides of Soybean Protein Induce Cell Cycle Arrest and Apoptosis on Human Oral Cancer Cell Line HSC-3 |
| title_short | The Hydrolytic Peptides of Soybean Protein Induce Cell Cycle Arrest and Apoptosis on Human Oral Cancer Cell Line HSC-3 |
| title_sort | hydrolytic peptides of soybean protein induce cell cycle arrest and apoptosis on human oral cancer cell line hsc 3 |
| topic | soy protein isolate anticancer peptides oral cancer antiproliferation cell cycle apoptosis |
| url | https://www.mdpi.com/1420-3049/27/9/2839 |
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