Hepatitis B genotype G and high frequency of lamivudine-resistance mutations among human immunodeficiencyvirus/hepatitis B virus co-infected patients in Brazil
In this study, we evaluated the hepatitis B virus (HBV) genotype distribution and HBV genomic mutations among a group of human immunodeficiency virus-HBV co-infected patients from an AIDS outpatient clinic in São Paulo. HBV serological markers were detected by commercially available enzyme immunoass...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2010-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600007 |
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| author | Adriana Cristina da Silva Ângela Maria Miranda Spina Marcílio Figueiredo Lemos Isabel Takano Oba Cristina de Fátima Guastini Michele Soares Gomes-Gouvêa João Renato Rebello Pinho Maria Cássia Jacintho Mendes-Correa |
| author_facet | Adriana Cristina da Silva Ângela Maria Miranda Spina Marcílio Figueiredo Lemos Isabel Takano Oba Cristina de Fátima Guastini Michele Soares Gomes-Gouvêa João Renato Rebello Pinho Maria Cássia Jacintho Mendes-Correa |
| author_sort | Adriana Cristina da Silva |
| collection | DOAJ |
| description | In this study, we evaluated the hepatitis B virus (HBV) genotype distribution and HBV genomic mutations among a group of human immunodeficiency virus-HBV co-infected patients from an AIDS outpatient clinic in São Paulo. HBV serological markers were detected by commercially available enzyme immunoassay kits. HBV DNA was detected using in-house nested polymerase chain reaction and quantified by Cobas Amplicor. HBV genotypes and mutations in the basal core promoter (BCP)/pre-core/core regions and surface/polymerase genes were determined by sequencing. Among the 59 patients included in this study, 55 reported prior use of lamivudine (LAM) or tenofovir. HBV DNA was detected in 16/22 patients, with a genotype distribution of A (n = 12,75%), G (n = 2,13%), D (n = 1,6%) and F (n = 1,6%). The sequence data of the two patients infected with genotype G strongly suggested co-infection with genotype A. In 10 patients with viremia, LAM-resistance mutations in the polymerase gene (rtL180M + rtM204V and rtV173L + rtL180M + rtM204V) were found, accompanied by changes in the envelope gene (sI195M, sW196L and sI195M/sE164D). Mutations in the BCP and pre-core regions were identified in four patients. In conclusion, genotype G, which is rarely seen in Brazil, was observed in the group of patients included in our study. A high prevalence of mutations associated with LAM-resistance and mutations associated with anti-HBs resistance were also found among these patients. |
| first_indexed | 2024-03-12T08:51:48Z |
| format | Article |
| id | doaj.art-4f574a104bde4b71affa204d56487642 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:51:48Z |
| publishDate | 2010-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-4f574a104bde4b71affa204d564876422023-09-02T16:17:44ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602010-09-01105677077810.1590/S0074-02762010000600007Hepatitis B genotype G and high frequency of lamivudine-resistance mutations among human immunodeficiencyvirus/hepatitis B virus co-infected patients in BrazilAdriana Cristina da SilvaÂngela Maria Miranda SpinaMarcílio Figueiredo LemosIsabel Takano ObaCristina de Fátima GuastiniMichele Soares Gomes-GouvêaJoão Renato Rebello PinhoMaria Cássia Jacintho Mendes-CorreaIn this study, we evaluated the hepatitis B virus (HBV) genotype distribution and HBV genomic mutations among a group of human immunodeficiency virus-HBV co-infected patients from an AIDS outpatient clinic in São Paulo. HBV serological markers were detected by commercially available enzyme immunoassay kits. HBV DNA was detected using in-house nested polymerase chain reaction and quantified by Cobas Amplicor. HBV genotypes and mutations in the basal core promoter (BCP)/pre-core/core regions and surface/polymerase genes were determined by sequencing. Among the 59 patients included in this study, 55 reported prior use of lamivudine (LAM) or tenofovir. HBV DNA was detected in 16/22 patients, with a genotype distribution of A (n = 12,75%), G (n = 2,13%), D (n = 1,6%) and F (n = 1,6%). The sequence data of the two patients infected with genotype G strongly suggested co-infection with genotype A. In 10 patients with viremia, LAM-resistance mutations in the polymerase gene (rtL180M + rtM204V and rtV173L + rtL180M + rtM204V) were found, accompanied by changes in the envelope gene (sI195M, sW196L and sI195M/sE164D). Mutations in the BCP and pre-core regions were identified in four patients. In conclusion, genotype G, which is rarely seen in Brazil, was observed in the group of patients included in our study. A high prevalence of mutations associated with LAM-resistance and mutations associated with anti-HBs resistance were also found among these patients.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600007hepatitis BHIVgenotypesgenotype Gmutationlamivudine |
| spellingShingle | Adriana Cristina da Silva Ângela Maria Miranda Spina Marcílio Figueiredo Lemos Isabel Takano Oba Cristina de Fátima Guastini Michele Soares Gomes-Gouvêa João Renato Rebello Pinho Maria Cássia Jacintho Mendes-Correa Hepatitis B genotype G and high frequency of lamivudine-resistance mutations among human immunodeficiencyvirus/hepatitis B virus co-infected patients in Brazil Memorias do Instituto Oswaldo Cruz hepatitis B HIV genotypes genotype G mutation lamivudine |
| title | Hepatitis B genotype G and high frequency of lamivudine-resistance mutations among human immunodeficiencyvirus/hepatitis B virus co-infected patients in Brazil |
| title_full | Hepatitis B genotype G and high frequency of lamivudine-resistance mutations among human immunodeficiencyvirus/hepatitis B virus co-infected patients in Brazil |
| title_fullStr | Hepatitis B genotype G and high frequency of lamivudine-resistance mutations among human immunodeficiencyvirus/hepatitis B virus co-infected patients in Brazil |
| title_full_unstemmed | Hepatitis B genotype G and high frequency of lamivudine-resistance mutations among human immunodeficiencyvirus/hepatitis B virus co-infected patients in Brazil |
| title_short | Hepatitis B genotype G and high frequency of lamivudine-resistance mutations among human immunodeficiencyvirus/hepatitis B virus co-infected patients in Brazil |
| title_sort | hepatitis b genotype g and high frequency of lamivudine resistance mutations among human immunodeficiencyvirus hepatitis b virus co infected patients in brazil |
| topic | hepatitis B HIV genotypes genotype G mutation lamivudine |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600007 |
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