Effectiveness of methods for cleaning arch wire: an in vitro study
The aim of this study was to evaluate various methods of removing bacterial and fungus biofilm, to simulate orthodontic arch wires cleaning before reinsertion in the patients appliance. Rectangular Nickel Titanium (NiTi), Stainless Steel (SS) and Titanium Molybdenum (TMA) wires were divided into fi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Uberlândia
2021-03-01
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| Series: | Bioscience Journal |
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| Online Access: | https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/55339 |
| _version_ | 1818157107376029696 |
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| author | Ana Carolina Portes Canongia Daniela Sales Alviano Moreno Leida Gomes Abraçado Matheus Melo Pithon Mônica Tirre Araújo |
| author_facet | Ana Carolina Portes Canongia Daniela Sales Alviano Moreno Leida Gomes Abraçado Matheus Melo Pithon Mônica Tirre Araújo |
| author_sort | Ana Carolina Portes Canongia |
| collection | DOAJ |
| description |
The aim of this study was to evaluate various methods of removing bacterial and fungus biofilm, to simulate orthodontic arch wires cleaning before reinsertion in the patients appliance. Rectangular Nickel Titanium (NiTi), Stainless Steel (SS) and Titanium Molybdenum (TMA) wires were divided into five groups, then contaminated with strains of Streptococcus mutans and Candida albicas. Four segments of each group served as control and were not contaminated. Six cleanings methods were used to remove the biofilm: cotton roll and a chemical agent (chlorhexidine, sodium hypochlorite, 70% alcohol), cotton roll and water, steel woll and immersion on enzymatic detergent. There was a control group not decontaminated Then wires were placed in broth separately, and after an incubation period the optical density (OD) was measured, observing whether there was microbial growth. A wire segment of each subgroup of SS 3M® was taken to the Scanning Electron Microscope (SEM) for visualization of the treatment response. The results were submitted to one-way ANOVA test and Tukey post-test. With the exception of 70% alcohol, the disinfection means behaved similarly regardless the type of wire. Two percent Chlorhexidine and 1% Sodium Hypochlorite totally removed the microorganisms while other agents left a high microbial concentration. Chemical cleaning is necessary to remove biofilm in orthodontic wires; 1% Sodium Hypochlorite and 2% Chlorhexidine are good disinfectants for this purpose.
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| first_indexed | 2024-12-11T15:08:56Z |
| format | Article |
| id | doaj.art-4f5a6ad0e87d4b7e9da7d28650c5a31e |
| institution | Directory Open Access Journal |
| issn | 1981-3163 |
| language | English |
| last_indexed | 2024-12-11T15:08:56Z |
| publishDate | 2021-03-01 |
| publisher | Universidade Federal de Uberlândia |
| record_format | Article |
| series | Bioscience Journal |
| spelling | doaj.art-4f5a6ad0e87d4b7e9da7d28650c5a31e2022-12-22T01:00:48ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632021-03-0137Effectiveness of methods for cleaning arch wire: an in vitro studyAna Carolina Portes Canongia0Daniela Sales Alviano Moreno1Leida Gomes Abraçado2Matheus Melo Pithon3Mônica Tirre Araújo4Universidade Federal do Rio de Janeiro Universidade Federal do Rio de Janeiro Centro Brasileiro de Pesquisas FísicasUniversidade Estadual do Sudoeste da BahiaUniversidade Federal do Rio de Janeiro The aim of this study was to evaluate various methods of removing bacterial and fungus biofilm, to simulate orthodontic arch wires cleaning before reinsertion in the patients appliance. Rectangular Nickel Titanium (NiTi), Stainless Steel (SS) and Titanium Molybdenum (TMA) wires were divided into five groups, then contaminated with strains of Streptococcus mutans and Candida albicas. Four segments of each group served as control and were not contaminated. Six cleanings methods were used to remove the biofilm: cotton roll and a chemical agent (chlorhexidine, sodium hypochlorite, 70% alcohol), cotton roll and water, steel woll and immersion on enzymatic detergent. There was a control group not decontaminated Then wires were placed in broth separately, and after an incubation period the optical density (OD) was measured, observing whether there was microbial growth. A wire segment of each subgroup of SS 3M® was taken to the Scanning Electron Microscope (SEM) for visualization of the treatment response. The results were submitted to one-way ANOVA test and Tukey post-test. With the exception of 70% alcohol, the disinfection means behaved similarly regardless the type of wire. Two percent Chlorhexidine and 1% Sodium Hypochlorite totally removed the microorganisms while other agents left a high microbial concentration. Chemical cleaning is necessary to remove biofilm in orthodontic wires; 1% Sodium Hypochlorite and 2% Chlorhexidine are good disinfectants for this purpose. https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/55339BacteriaHygieneOrthodontics. |
| spellingShingle | Ana Carolina Portes Canongia Daniela Sales Alviano Moreno Leida Gomes Abraçado Matheus Melo Pithon Mônica Tirre Araújo Effectiveness of methods for cleaning arch wire: an in vitro study Bioscience Journal Bacteria Hygiene Orthodontics. |
| title | Effectiveness of methods for cleaning arch wire: an in vitro study |
| title_full | Effectiveness of methods for cleaning arch wire: an in vitro study |
| title_fullStr | Effectiveness of methods for cleaning arch wire: an in vitro study |
| title_full_unstemmed | Effectiveness of methods for cleaning arch wire: an in vitro study |
| title_short | Effectiveness of methods for cleaning arch wire: an in vitro study |
| title_sort | effectiveness of methods for cleaning arch wire an in vitro study |
| topic | Bacteria Hygiene Orthodontics. |
| url | https://seer-dev.ufu.br/index.php/biosciencejournal/article/view/55339 |
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