Development of novel ELISA based on tyramine signal amplification for sensitive detection of danofloxacin
ObjectiveTo develop a novel ELISA for sensitive and naked-eye detection of danofloxacin （DAN）.MethodsBased on TYR signal amplification， a novel ELISA was developed for sensitive detection of DAN. Analytical performance of the method was conducted by sensitivity and specificity experiments. The metho...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
The Editorial Office of Chinese Journal of Food Hygiene
2022-05-01
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| Series: | Zhongguo shipin weisheng zazhi |
| Subjects: | |
| Online Access: | http://www.zgspws.com/zgspwszz/article/abstract/202203019?st=article_issue |
| Summary: | ObjectiveTo develop a novel ELISA for sensitive and naked-eye detection of danofloxacin （DAN）.MethodsBased on TYR signal amplification， a novel ELISA was developed for sensitive detection of DAN. Analytical performance of the method was conducted by sensitivity and specificity experiments. The method was used for detecting DAN-spiked chicken and pork samples to verify.ResultsThis method could be used to qualitatively detect DAN with naked eyes at a cut-off value of 0.4 ng/mL， which was 25-fold lower than that of the traditional ELISA （10 ng/mL）. The method exhibited no cross-reaction of DAN with six other drugs in buffer. The method was successfully used for detecting DAN-spiked chicken and pork samples.ConclusionThe developed novel ELISA exhibits high sensitivity and specificity， and can be used for the danofloxacin detection in actual samples by naked eye. |
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| ISSN: | 1004-8456 |