The Homeodomain-Leucine Zipper Genes Family Regulates the Jinggangmycin Mediated Immune Response of <i>Oryza sativa</i> to <i>Nilaparvata lugens</i>, and <i>Laodelphax striatellus</i>
The homeodomain-leucine zipper (HDZIP) is an important transcription factor family, instrumental not only in growth but in finetuning plant responses to environmental adversaries. Despite the plethora of literature available, the role of HDZIP genes under chewing and sucking insects remains elusive....
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MDPI AG
2022-08-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/9/8/398 |
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| author | Sheraz Ahmad Yu Chen Amir Zaman Shah Huaiqi Wang Chuanyuan Xi Haowen Zhu Linquan Ge |
| author_facet | Sheraz Ahmad Yu Chen Amir Zaman Shah Huaiqi Wang Chuanyuan Xi Haowen Zhu Linquan Ge |
| author_sort | Sheraz Ahmad |
| collection | DOAJ |
| description | The homeodomain-leucine zipper (HDZIP) is an important transcription factor family, instrumental not only in growth but in finetuning plant responses to environmental adversaries. Despite the plethora of literature available, the role of HDZIP genes under chewing and sucking insects remains elusive. Herein, we identified 40 <i>OsHDZIP</i> genes from the rice genome database. The evolutionary relationship, gene structure, conserved motifs, and chemical properties highlight the key aspects of <i>OsHDZIP</i> genes in rice. The <i>OsHDZIP</i> family is divided into a further four subfamilies (i.e., HDZIP I, HDZIP II, HDZIP III, and HDZIP IV). Moreover, the protein–protein interaction and Gene Ontology (GO) analysis showed that <i>OsHDZIP</i> genes regulate plant growth and response to various environmental stimuli. Various microRNA (miRNA) families targeted HDZIP III subfamily genes. The microarray data analysis showed that <i>OsHDZIP</i> was expressed in almost all tested tissues. Additionally, the differential expression patterns of the <i>OsHDZIP</i> genes were found under salinity stress and hormonal treatments, whereas under brown planthopper (BPH), striped stem borer (SSB), and rice leaf folder (RLF), only <i>OsHDZIP3</i>, <i>OsHDZIP4</i>, <i>OsHDZIP40</i>, <i>OsHDZIP10</i>, and <i>OsHDZIP20</i> displayed expression. The qRT-PCR analysis further validated the expression of <i>OsHDZIP20</i>, <i>OsHDZIP40</i>, and <i>OsHDZIP10</i> under BPH, small brown planthopper (SBPH) infestations, and jinggangmycin (JGM) spraying applications. Our results provide detailed knowledge of the <i>OsHDZIP</i> gene family resistance in rice plants and will facilitate the development of stress-resilient cultivars, particularly against chewing and sucking insect pests. |
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| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T10:01:24Z |
| publishDate | 2022-08-01 |
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| spelling | doaj.art-4f5be18921fd4ba3a586b53af16b9c762023-12-01T23:25:25ZengMDPI AGBioengineering2306-53542022-08-019839810.3390/bioengineering9080398The Homeodomain-Leucine Zipper Genes Family Regulates the Jinggangmycin Mediated Immune Response of <i>Oryza sativa</i> to <i>Nilaparvata lugens</i>, and <i>Laodelphax striatellus</i>Sheraz Ahmad0Yu Chen1Amir Zaman Shah2Huaiqi Wang3Chuanyuan Xi4Haowen Zhu5Linquan Ge6College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, ChinaThe homeodomain-leucine zipper (HDZIP) is an important transcription factor family, instrumental not only in growth but in finetuning plant responses to environmental adversaries. Despite the plethora of literature available, the role of HDZIP genes under chewing and sucking insects remains elusive. Herein, we identified 40 <i>OsHDZIP</i> genes from the rice genome database. The evolutionary relationship, gene structure, conserved motifs, and chemical properties highlight the key aspects of <i>OsHDZIP</i> genes in rice. The <i>OsHDZIP</i> family is divided into a further four subfamilies (i.e., HDZIP I, HDZIP II, HDZIP III, and HDZIP IV). Moreover, the protein–protein interaction and Gene Ontology (GO) analysis showed that <i>OsHDZIP</i> genes regulate plant growth and response to various environmental stimuli. Various microRNA (miRNA) families targeted HDZIP III subfamily genes. The microarray data analysis showed that <i>OsHDZIP</i> was expressed in almost all tested tissues. Additionally, the differential expression patterns of the <i>OsHDZIP</i> genes were found under salinity stress and hormonal treatments, whereas under brown planthopper (BPH), striped stem borer (SSB), and rice leaf folder (RLF), only <i>OsHDZIP3</i>, <i>OsHDZIP4</i>, <i>OsHDZIP40</i>, <i>OsHDZIP10</i>, and <i>OsHDZIP20</i> displayed expression. The qRT-PCR analysis further validated the expression of <i>OsHDZIP20</i>, <i>OsHDZIP40</i>, and <i>OsHDZIP10</i> under BPH, small brown planthopper (SBPH) infestations, and jinggangmycin (JGM) spraying applications. Our results provide detailed knowledge of the <i>OsHDZIP</i> gene family resistance in rice plants and will facilitate the development of stress-resilient cultivars, particularly against chewing and sucking insect pests.https://www.mdpi.com/2306-5354/9/8/398homeodomain-leucine zippertranscriptomic analysisbrown planthoppersmall brown planthopperjinggangmycin |
| spellingShingle | Sheraz Ahmad Yu Chen Amir Zaman Shah Huaiqi Wang Chuanyuan Xi Haowen Zhu Linquan Ge The Homeodomain-Leucine Zipper Genes Family Regulates the Jinggangmycin Mediated Immune Response of <i>Oryza sativa</i> to <i>Nilaparvata lugens</i>, and <i>Laodelphax striatellus</i> Bioengineering homeodomain-leucine zipper transcriptomic analysis brown planthopper small brown planthopper jinggangmycin |
| title | The Homeodomain-Leucine Zipper Genes Family Regulates the Jinggangmycin Mediated Immune Response of <i>Oryza sativa</i> to <i>Nilaparvata lugens</i>, and <i>Laodelphax striatellus</i> |
| title_full | The Homeodomain-Leucine Zipper Genes Family Regulates the Jinggangmycin Mediated Immune Response of <i>Oryza sativa</i> to <i>Nilaparvata lugens</i>, and <i>Laodelphax striatellus</i> |
| title_fullStr | The Homeodomain-Leucine Zipper Genes Family Regulates the Jinggangmycin Mediated Immune Response of <i>Oryza sativa</i> to <i>Nilaparvata lugens</i>, and <i>Laodelphax striatellus</i> |
| title_full_unstemmed | The Homeodomain-Leucine Zipper Genes Family Regulates the Jinggangmycin Mediated Immune Response of <i>Oryza sativa</i> to <i>Nilaparvata lugens</i>, and <i>Laodelphax striatellus</i> |
| title_short | The Homeodomain-Leucine Zipper Genes Family Regulates the Jinggangmycin Mediated Immune Response of <i>Oryza sativa</i> to <i>Nilaparvata lugens</i>, and <i>Laodelphax striatellus</i> |
| title_sort | homeodomain leucine zipper genes family regulates the jinggangmycin mediated immune response of i oryza sativa i to i nilaparvata lugens i and i laodelphax striatellus i |
| topic | homeodomain-leucine zipper transcriptomic analysis brown planthopper small brown planthopper jinggangmycin |
| url | https://www.mdpi.com/2306-5354/9/8/398 |
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