The effect of hepatopancreas homogenate of the Red king crab on HA-based filler
In this study, several methods were used to analyze the hydrolysis of hyaluronic acid (HA)-based cosmetic fillers by the hepatopancreas homogenate of the Red king crab. The results show that the homogenate and commercially available hyaluronidases have similar hydrolysis activities on the fillers. A...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2020-02-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/8579.pdf |
| _version_ | 1797419694762754048 |
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| author | Tatyana Ponomareva Dmitrii Sliadovskii Maria Timchenko Maxim Molchanov Alexander Timchenko Evgeny Sogorin |
| author_facet | Tatyana Ponomareva Dmitrii Sliadovskii Maria Timchenko Maxim Molchanov Alexander Timchenko Evgeny Sogorin |
| author_sort | Tatyana Ponomareva |
| collection | DOAJ |
| description | In this study, several methods were used to analyze the hydrolysis of hyaluronic acid (HA)-based cosmetic fillers by the hepatopancreas homogenate of the Red king crab. The results show that the homogenate and commercially available hyaluronidases have similar hydrolysis activities on the fillers. Atomic force microscopy images reveal that the HA fillers consist mainly of spherical-like particles, which are converted into filamentous structures as a result of hydrolysis by the Red king crab hepatopancreas homogenate. Turbidimetric analysis of the hydrolysis process shows that HA aggregation with acidic albumin exhibits a bell-shaped dependence on reaction time. Analysis of the hydrolysis process by nuclear magnetic resonance shows that HA degradation lasts several days. The maximum rate of the reaction is detected in the 1st h of incubation. The data confirm that the purified homogenate of the Red king crab hepatopancreas exerts hyaluronidase activity on HA-based cosmetic fillers; therefore, it may be considered as a potential therapeutic agent for treating filler complications. |
| first_indexed | 2024-03-09T06:52:00Z |
| format | Article |
| id | doaj.art-4f5f687cb7e84fad9b076907f8ecb448 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:52:00Z |
| publishDate | 2020-02-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-4f5f687cb7e84fad9b076907f8ecb4482023-12-03T10:24:49ZengPeerJ Inc.PeerJ2167-83592020-02-018e857910.7717/peerj.8579The effect of hepatopancreas homogenate of the Red king crab on HA-based fillerTatyana Ponomareva0Dmitrii Sliadovskii1Maria Timchenko2Maxim Molchanov3Alexander Timchenko4Evgeny Sogorin5Federal Research Center “Pushchino Scientific Center for Biological Research of the RAS”, Pushchino, RussiaFederal Research Center “Pushchino Scientific Center for Biological Research of the RAS”, Pushchino, RussiaFederal Research Center “Pushchino Scientific Center for Biological Research of the RAS”, Pushchino, RussiaInstitute of Theoretical and Experimental Biophysics of the RAS, Pushchino, RussiaInstitute of Protein Research of the RAS, Pushchino, RussiaFederal Research Center “Pushchino Scientific Center for Biological Research of the RAS”, Pushchino, RussiaIn this study, several methods were used to analyze the hydrolysis of hyaluronic acid (HA)-based cosmetic fillers by the hepatopancreas homogenate of the Red king crab. The results show that the homogenate and commercially available hyaluronidases have similar hydrolysis activities on the fillers. Atomic force microscopy images reveal that the HA fillers consist mainly of spherical-like particles, which are converted into filamentous structures as a result of hydrolysis by the Red king crab hepatopancreas homogenate. Turbidimetric analysis of the hydrolysis process shows that HA aggregation with acidic albumin exhibits a bell-shaped dependence on reaction time. Analysis of the hydrolysis process by nuclear magnetic resonance shows that HA degradation lasts several days. The maximum rate of the reaction is detected in the 1st h of incubation. The data confirm that the purified homogenate of the Red king crab hepatopancreas exerts hyaluronidase activity on HA-based cosmetic fillers; therefore, it may be considered as a potential therapeutic agent for treating filler complications.https://peerj.com/articles/8579.pdfTreating filler complicationsHyaluronidaseRed king crabHepatopancreasHyaluronic acidHyaluronidase activity |
| spellingShingle | Tatyana Ponomareva Dmitrii Sliadovskii Maria Timchenko Maxim Molchanov Alexander Timchenko Evgeny Sogorin The effect of hepatopancreas homogenate of the Red king crab on HA-based filler PeerJ Treating filler complications Hyaluronidase Red king crab Hepatopancreas Hyaluronic acid Hyaluronidase activity |
| title | The effect of hepatopancreas homogenate of the Red king crab on HA-based filler |
| title_full | The effect of hepatopancreas homogenate of the Red king crab on HA-based filler |
| title_fullStr | The effect of hepatopancreas homogenate of the Red king crab on HA-based filler |
| title_full_unstemmed | The effect of hepatopancreas homogenate of the Red king crab on HA-based filler |
| title_short | The effect of hepatopancreas homogenate of the Red king crab on HA-based filler |
| title_sort | effect of hepatopancreas homogenate of the red king crab on ha based filler |
| topic | Treating filler complications Hyaluronidase Red king crab Hepatopancreas Hyaluronic acid Hyaluronidase activity |
| url | https://peerj.com/articles/8579.pdf |
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