Accuracy of a rapid diagnosis test, microscopy and loop-mediated isothermal amplification in the detection of asymptomatic Plasmodium infections in Korhogo, Northern Côte d’Ivoire
Abstract Background Highly sensitive and accurate malaria diagnostic tools are essential to identify asymptomatic low parasitaemia infections. This study evaluated the performance of histidine-rich protein 2 (HRP-2) based rapid diagnostic tests (RDTs), microscopy and loop-mediated isothermal amplifi...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-04-01
|
| Series: | Malaria Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12936-022-04133-6 |
| _version_ | 1818354995560448000 |
|---|---|
| author | Edjronké M. A. Benié Kigbafori D. Silué Xavier C. Ding Issa Yeo J. B. Assamoi Karim Tuo Akpa P. Gnagne Lasme J. C. E. Esso Jean T. Coulibaly Serge-Brice Assi Bassirou Bonfoh William Yavo Eliézer K. N’Goran |
| author_facet | Edjronké M. A. Benié Kigbafori D. Silué Xavier C. Ding Issa Yeo J. B. Assamoi Karim Tuo Akpa P. Gnagne Lasme J. C. E. Esso Jean T. Coulibaly Serge-Brice Assi Bassirou Bonfoh William Yavo Eliézer K. N’Goran |
| author_sort | Edjronké M. A. Benié |
| collection | DOAJ |
| description | Abstract Background Highly sensitive and accurate malaria diagnostic tools are essential to identify asymptomatic low parasitaemia infections. This study evaluated the performance of histidine-rich protein 2 (HRP-2) based rapid diagnostic tests (RDTs), microscopy and loop-mediated isothermal amplification (LAMP) for the detection of asymptomatic Plasmodium spp. infections in Northern Côte d’Ivoire, using nested polymerase chain reaction (nPCR) as reference. Methods A household-based survey was carried out in July 2016, in the health district of Korhogo, involving 1011 adults without malaria symptom nor history of fever during the week before recruitment. The fresh capillary blood samples were collected to detect Plasmodium infections using on HRP-2-based RDTs, microscopy and LAMP and stored as dried blood spots (DBS). A subset of the DBS (247/1011, 24.4%) was randomly selected for nPCR analyses. Additionally, venous blood samples, according to LAMP result (45 LAMP positive and 65 LAMP negative) were collected among the included participants to perform the nested PCR used as the reference. Results The prevalence of asymptomatic Plasmodium spp. infections determined by RDT, microscopy, and LAMP were 4% (95% confidence interval (CI) 2.8–5.3), 5.2% (95% CI 3.9–6.6) and 18.8% (95% CI 16.4–21.2), respectively. Considering PCR on venous blood as reference, performed on 110 samples, the sensibility and specificity were, respectively, 17.8% (95% CI 6.1–29.4) and 100% for RDT, 20.0% (95% CI 7.8–32) and 100% for microscopy, and 93.3% (95% CI 85.7–100) and 95.4% (95% CI 92.2–100) for LAMP. Conclusion In Northern Côte d’Ivoire, asymptomatic Plasmodium infection was found to be widely distributed as approximately one out of five study participants was found to be Plasmodium infected. LAMP appears currently to be the only available diagnostic method that can identify in the field this reservoir of infections and should be the method to consider for potential future active case detection interventions targeting elimination of these infections. |
| first_indexed | 2024-12-13T19:34:17Z |
| format | Article |
| id | doaj.art-4f5fec9be69f47edb93e789ab6bcb281 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-13T19:34:17Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-4f5fec9be69f47edb93e789ab6bcb2812022-12-21T23:33:51ZengBMCMalaria Journal1475-28752022-04-0121111010.1186/s12936-022-04133-6Accuracy of a rapid diagnosis test, microscopy and loop-mediated isothermal amplification in the detection of asymptomatic Plasmodium infections in Korhogo, Northern Côte d’IvoireEdjronké M. A. Benié0Kigbafori D. Silué1Xavier C. Ding2Issa Yeo3J. B. Assamoi4Karim Tuo5Akpa P. Gnagne6Lasme J. C. E. Esso7Jean T. Coulibaly8Serge-Brice Assi9Bassirou Bonfoh10William Yavo11Eliézer K. N’Goran12Unité de Formation et de Recherche Biosciences, Laboratoire de Biologie et Santé, Université Félix Houphouët-BoignyUnité de Formation et de Recherche Biosciences, Laboratoire de Biologie et Santé, Université Félix Houphouët-BoignyFIND, Campus BiotechUnité de Formation et de Recherche Biosciences, Laboratoire de Biologie et Santé, Université Félix Houphouët-BoignyCentre Suisse de Recherches Scientifiques en Côte d’IvoireInstitut Pasteur de Côte d’IvoireCentre de Recherche et de Lutte Contre le Paludisme, Institut National de Santé PubliqueCentre Suisse de Recherches Scientifiques en Côte d’IvoireUnité de Formation et de Recherche Biosciences, Laboratoire de Biologie et Santé, Université Félix Houphouët-BoignyProgramme National de Lutte Contre le Paludisme en Côte d’Ivoire, Ministère de la Santé et de l’Hygiène PubliqueCentre Suisse de Recherches Scientifiques en Côte d’IvoireCentre de Recherche et de Lutte Contre le Paludisme, Institut National de Santé PubliqueUnité de Formation et de Recherche Biosciences, Laboratoire de Biologie et Santé, Université Félix Houphouët-BoignyAbstract Background Highly sensitive and accurate malaria diagnostic tools are essential to identify asymptomatic low parasitaemia infections. This study evaluated the performance of histidine-rich protein 2 (HRP-2) based rapid diagnostic tests (RDTs), microscopy and loop-mediated isothermal amplification (LAMP) for the detection of asymptomatic Plasmodium spp. infections in Northern Côte d’Ivoire, using nested polymerase chain reaction (nPCR) as reference. Methods A household-based survey was carried out in July 2016, in the health district of Korhogo, involving 1011 adults without malaria symptom nor history of fever during the week before recruitment. The fresh capillary blood samples were collected to detect Plasmodium infections using on HRP-2-based RDTs, microscopy and LAMP and stored as dried blood spots (DBS). A subset of the DBS (247/1011, 24.4%) was randomly selected for nPCR analyses. Additionally, venous blood samples, according to LAMP result (45 LAMP positive and 65 LAMP negative) were collected among the included participants to perform the nested PCR used as the reference. Results The prevalence of asymptomatic Plasmodium spp. infections determined by RDT, microscopy, and LAMP were 4% (95% confidence interval (CI) 2.8–5.3), 5.2% (95% CI 3.9–6.6) and 18.8% (95% CI 16.4–21.2), respectively. Considering PCR on venous blood as reference, performed on 110 samples, the sensibility and specificity were, respectively, 17.8% (95% CI 6.1–29.4) and 100% for RDT, 20.0% (95% CI 7.8–32) and 100% for microscopy, and 93.3% (95% CI 85.7–100) and 95.4% (95% CI 92.2–100) for LAMP. Conclusion In Northern Côte d’Ivoire, asymptomatic Plasmodium infection was found to be widely distributed as approximately one out of five study participants was found to be Plasmodium infected. LAMP appears currently to be the only available diagnostic method that can identify in the field this reservoir of infections and should be the method to consider for potential future active case detection interventions targeting elimination of these infections.https://doi.org/10.1186/s12936-022-04133-6Plasmodium spp.AsymptomaticRDTMicroscopyLAMPPCR |
| spellingShingle | Edjronké M. A. Benié Kigbafori D. Silué Xavier C. Ding Issa Yeo J. B. Assamoi Karim Tuo Akpa P. Gnagne Lasme J. C. E. Esso Jean T. Coulibaly Serge-Brice Assi Bassirou Bonfoh William Yavo Eliézer K. N’Goran Accuracy of a rapid diagnosis test, microscopy and loop-mediated isothermal amplification in the detection of asymptomatic Plasmodium infections in Korhogo, Northern Côte d’Ivoire Malaria Journal Plasmodium spp. Asymptomatic RDT Microscopy LAMP PCR |
| title | Accuracy of a rapid diagnosis test, microscopy and loop-mediated isothermal amplification in the detection of asymptomatic Plasmodium infections in Korhogo, Northern Côte d’Ivoire |
| title_full | Accuracy of a rapid diagnosis test, microscopy and loop-mediated isothermal amplification in the detection of asymptomatic Plasmodium infections in Korhogo, Northern Côte d’Ivoire |
| title_fullStr | Accuracy of a rapid diagnosis test, microscopy and loop-mediated isothermal amplification in the detection of asymptomatic Plasmodium infections in Korhogo, Northern Côte d’Ivoire |
| title_full_unstemmed | Accuracy of a rapid diagnosis test, microscopy and loop-mediated isothermal amplification in the detection of asymptomatic Plasmodium infections in Korhogo, Northern Côte d’Ivoire |
| title_short | Accuracy of a rapid diagnosis test, microscopy and loop-mediated isothermal amplification in the detection of asymptomatic Plasmodium infections in Korhogo, Northern Côte d’Ivoire |
| title_sort | accuracy of a rapid diagnosis test microscopy and loop mediated isothermal amplification in the detection of asymptomatic plasmodium infections in korhogo northern cote d ivoire |
| topic | Plasmodium spp. Asymptomatic RDT Microscopy LAMP PCR |
| url | https://doi.org/10.1186/s12936-022-04133-6 |
| work_keys_str_mv | AT edjronkemabenie accuracyofarapiddiagnosistestmicroscopyandloopmediatedisothermalamplificationinthedetectionofasymptomaticplasmodiuminfectionsinkorhogonortherncotedivoire AT kigbaforidsilue accuracyofarapiddiagnosistestmicroscopyandloopmediatedisothermalamplificationinthedetectionofasymptomaticplasmodiuminfectionsinkorhogonortherncotedivoire AT xaviercding accuracyofarapiddiagnosistestmicroscopyandloopmediatedisothermalamplificationinthedetectionofasymptomaticplasmodiuminfectionsinkorhogonortherncotedivoire AT issayeo accuracyofarapiddiagnosistestmicroscopyandloopmediatedisothermalamplificationinthedetectionofasymptomaticplasmodiuminfectionsinkorhogonortherncotedivoire AT jbassamoi accuracyofarapiddiagnosistestmicroscopyandloopmediatedisothermalamplificationinthedetectionofasymptomaticplasmodiuminfectionsinkorhogonortherncotedivoire AT karimtuo accuracyofarapiddiagnosistestmicroscopyandloopmediatedisothermalamplificationinthedetectionofasymptomaticplasmodiuminfectionsinkorhogonortherncotedivoire AT akpapgnagne accuracyofarapiddiagnosistestmicroscopyandloopmediatedisothermalamplificationinthedetectionofasymptomaticplasmodiuminfectionsinkorhogonortherncotedivoire AT lasmejceesso accuracyofarapiddiagnosistestmicroscopyandloopmediatedisothermalamplificationinthedetectionofasymptomaticplasmodiuminfectionsinkorhogonortherncotedivoire AT jeantcoulibaly accuracyofarapiddiagnosistestmicroscopyandloopmediatedisothermalamplificationinthedetectionofasymptomaticplasmodiuminfectionsinkorhogonortherncotedivoire AT sergebriceassi accuracyofarapiddiagnosistestmicroscopyandloopmediatedisothermalamplificationinthedetectionofasymptomaticplasmodiuminfectionsinkorhogonortherncotedivoire AT bassiroubonfoh accuracyofarapiddiagnosistestmicroscopyandloopmediatedisothermalamplificationinthedetectionofasymptomaticplasmodiuminfectionsinkorhogonortherncotedivoire AT williamyavo accuracyofarapiddiagnosistestmicroscopyandloopmediatedisothermalamplificationinthedetectionofasymptomaticplasmodiuminfectionsinkorhogonortherncotedivoire AT eliezerkngoran accuracyofarapiddiagnosistestmicroscopyandloopmediatedisothermalamplificationinthedetectionofasymptomaticplasmodiuminfectionsinkorhogonortherncotedivoire |