DETECTION OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS RNA IN BIOLOGICAL SAMPLES BY REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION
Aim. Detection and identification of Venezuelan equine encephalomyelitis (VEE) virus RNA in biological samples by reverse-transcription polymerase chain reaction (RT-PCR) and RT-PCR in real time (rRT-PCR). Materials and methods. VEE, Sindbis, West Nile, Japanese and tick-borne encephalitis viruses w...
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2015-12-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.crie.ru/jour/article/view/14150 |
| _version_ | 1797782200693817344 |
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| author | A. A Petrov N. S Pyshnaya V. N Lebedev V. S Kulish L. F Stovba A. V Kazantsev S. V Borisevich |
| author_facet | A. A Petrov N. S Pyshnaya V. N Lebedev V. S Kulish L. F Stovba A. V Kazantsev S. V Borisevich |
| author_sort | A. A Petrov |
| collection | DOAJ |
| description | Aim. Detection and identification of Venezuelan equine encephalomyelitis (VEE) virus RNA in biological samples by reverse-transcription polymerase chain reaction (RT-PCR) and RT-PCR in real time (rRT-PCR). Materials and methods. VEE, Sindbis, West Nile, Japanese and tick-borne encephalitis viruses were studied. Cell culture of chicken fibroblasts, outbred mice and rats, Javanese macaques were used in the experiments. Biological activity determination of the running culture of causative agents used in the experiments was carried out by negative colony method in monolayer cell culture under agar coating and using intra-cerebral infection of mice. Reagent kits developed in the 48th Central Research Institute and Institute of Analytical Instrument Engineering were used during execution of experiments of VEE virus RNA detection by RT-PCR and rRT-PCR. Results. VEE virus was detected in biological samples by various methods. Data from RT-PCR and rRT-PCR are in accordance with the results of virus detection in samples using sensitive animals. Conclusion. Use of molecular-diagnostics methods for detection in biological samples of a causative agent of a dangerous infectious disease is important for procuring biological safety of Russian Federation. |
| first_indexed | 2024-03-13T00:07:39Z |
| format | Article |
| id | doaj.art-4f625a5e084a4e79b933f08160f21809 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-03-13T00:07:39Z |
| publishDate | 2015-12-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-4f625a5e084a4e79b933f08160f218092023-07-12T20:21:14ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132015-12-0192682862575DETECTION OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS RNA IN BIOLOGICAL SAMPLES BY REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTIONA. A Petrov0N. S Pyshnaya1V. N Lebedev2V. S Kulish3L. F Stovba4A. V Kazantsev5S. V Borisevich648th Research Institute of the Ministry of Defense of Russian Federation48th Research Institute of the Ministry of Defense of Russian Federation48th Research Institute of the Ministry of Defense of Russian Federation48th Research Institute of the Ministry of Defense of Russian Federation48th Research Institute of the Ministry of Defense of Russian Federation48th Research Institute of the Ministry of Defense of Russian Federation48th Research Institute of the Ministry of Defense of Russian FederationAim. Detection and identification of Venezuelan equine encephalomyelitis (VEE) virus RNA in biological samples by reverse-transcription polymerase chain reaction (RT-PCR) and RT-PCR in real time (rRT-PCR). Materials and methods. VEE, Sindbis, West Nile, Japanese and tick-borne encephalitis viruses were studied. Cell culture of chicken fibroblasts, outbred mice and rats, Javanese macaques were used in the experiments. Biological activity determination of the running culture of causative agents used in the experiments was carried out by negative colony method in monolayer cell culture under agar coating and using intra-cerebral infection of mice. Reagent kits developed in the 48th Central Research Institute and Institute of Analytical Instrument Engineering were used during execution of experiments of VEE virus RNA detection by RT-PCR and rRT-PCR. Results. VEE virus was detected in biological samples by various methods. Data from RT-PCR and rRT-PCR are in accordance with the results of virus detection in samples using sensitive animals. Conclusion. Use of molecular-diagnostics methods for detection in biological samples of a causative agent of a dangerous infectious disease is important for procuring biological safety of Russian Federation.https://microbiol.crie.ru/jour/article/view/14150vee virusrnareverse transcriptionpcrbiological samplelaboratory animalsexperimental infection |
| spellingShingle | A. A Petrov N. S Pyshnaya V. N Lebedev V. S Kulish L. F Stovba A. V Kazantsev S. V Borisevich DETECTION OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS RNA IN BIOLOGICAL SAMPLES BY REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION Журнал микробиологии, эпидемиологии и иммунобиологии vee virus rna reverse transcription pcr biological sample laboratory animals experimental infection |
| title | DETECTION OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS RNA IN BIOLOGICAL SAMPLES BY REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION |
| title_full | DETECTION OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS RNA IN BIOLOGICAL SAMPLES BY REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION |
| title_fullStr | DETECTION OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS RNA IN BIOLOGICAL SAMPLES BY REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION |
| title_full_unstemmed | DETECTION OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS RNA IN BIOLOGICAL SAMPLES BY REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION |
| title_short | DETECTION OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS RNA IN BIOLOGICAL SAMPLES BY REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION |
| title_sort | detection of venezuelan equine encephalomyelitis virus rna in biological samples by reverse transcription polymerase chain reaction |
| topic | vee virus rna reverse transcription pcr biological sample laboratory animals experimental infection |
| url | https://microbiol.crie.ru/jour/article/view/14150 |
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