Real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pH

Abstract A biopsy is often performed for the diagnosis of cancer during a surgical operation. In addition, pathological biopsy is required to discriminate the margin between cancer tissues and normal tissues in surgical specimens. In this study, we presented a novel method for discriminating between...

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Main Authors: Jooran Lee, Byungyeon Kim, Byungjun Park, Youngjae Won, Sang-Yeob Kim, Seungrag Lee
Format: Article
Language:English
Published: Nature Portfolio 2021-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-96531-0
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author Jooran Lee
Byungyeon Kim
Byungjun Park
Youngjae Won
Sang-Yeob Kim
Seungrag Lee
author_facet Jooran Lee
Byungyeon Kim
Byungjun Park
Youngjae Won
Sang-Yeob Kim
Seungrag Lee
author_sort Jooran Lee
collection DOAJ
description Abstract A biopsy is often performed for the diagnosis of cancer during a surgical operation. In addition, pathological biopsy is required to discriminate the margin between cancer tissues and normal tissues in surgical specimens. In this study, we presented a novel method for discriminating between tumor and normal tissues using fluorescence lifetime endoscopy (FLE). We demonstrated the relationship between the fluorescence lifetime and pH in fluorescein using the proposed fluorescence lifetime measurement system. We also showed that cancer could be diagnosed based on this relationship by assessing differences in pH based fluorescence lifetime between cancer and normal tissues using two different types of tumor such as breast tumors (MDA-MB-361) and skin tumors (A375), where cancer tissues have ranged in pH from 4.5 to 7.0 and normal tissues have ranged in pH from 7.0 to 7.4. To support this approach, we performed hematoxylin and eosin (H&E) staining test of normal and cancer tissues within a certain area. From these results, we showed the ability to diagnose a cancer using FLE technique, which were consistent with the diagnosis of a cancer with H&E staining test. In summary, the proposed pH-based FLE technique could provide a real time, in vivo, and in-situ clinical diagnostic method for the cancer surgical and could be presented as an alternative to biopsy procedures.
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spelling doaj.art-4f714369ab7e42958b806d64aaf49cca2022-12-21T22:59:36ZengNature PortfolioScientific Reports2045-23222021-08-0111111210.1038/s41598-021-96531-0Real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pHJooran Lee0Byungyeon Kim1Byungjun Park2Youngjae Won3Sang-Yeob Kim4Seungrag Lee5Medical Device Development Center, Osong Medical Innovation FoundationMedical Device Development Center, Osong Medical Innovation FoundationMedical Device Development Center, Osong Medical Innovation FoundationMedical Device Development Center, Osong Medical Innovation FoundationAsan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of MedicineMedical Device Development Center, Osong Medical Innovation FoundationAbstract A biopsy is often performed for the diagnosis of cancer during a surgical operation. In addition, pathological biopsy is required to discriminate the margin between cancer tissues and normal tissues in surgical specimens. In this study, we presented a novel method for discriminating between tumor and normal tissues using fluorescence lifetime endoscopy (FLE). We demonstrated the relationship between the fluorescence lifetime and pH in fluorescein using the proposed fluorescence lifetime measurement system. We also showed that cancer could be diagnosed based on this relationship by assessing differences in pH based fluorescence lifetime between cancer and normal tissues using two different types of tumor such as breast tumors (MDA-MB-361) and skin tumors (A375), where cancer tissues have ranged in pH from 4.5 to 7.0 and normal tissues have ranged in pH from 7.0 to 7.4. To support this approach, we performed hematoxylin and eosin (H&E) staining test of normal and cancer tissues within a certain area. From these results, we showed the ability to diagnose a cancer using FLE technique, which were consistent with the diagnosis of a cancer with H&E staining test. In summary, the proposed pH-based FLE technique could provide a real time, in vivo, and in-situ clinical diagnostic method for the cancer surgical and could be presented as an alternative to biopsy procedures.https://doi.org/10.1038/s41598-021-96531-0
spellingShingle Jooran Lee
Byungyeon Kim
Byungjun Park
Youngjae Won
Sang-Yeob Kim
Seungrag Lee
Real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pH
Scientific Reports
title Real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pH
title_full Real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pH
title_fullStr Real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pH
title_full_unstemmed Real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pH
title_short Real-time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the pH
title_sort real time cancer diagnosis of breast cancer using fluorescence lifetime endoscopy based on the ph
url https://doi.org/10.1038/s41598-021-96531-0
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