Synthesis and characterization of natural biomaterial composite nanofibers for ocular drug delivery systems
Due to its numerous reported benefits, including biomimetic, immunomodulation, and compatibility with tissue microenvironment, natural bio composite nanomaterials are in demand as an effective replacement in the field of tissue engineering. As a result, in this study, we investigated polysaccharides...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | OpenNano |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352952023000026 |
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| author | Jayavigneeswari Suresh babu Aravindan Saravanan Bharathselvi Muthuvel Ronnie George Janakiraman Narayanan |
| author_facet | Jayavigneeswari Suresh babu Aravindan Saravanan Bharathselvi Muthuvel Ronnie George Janakiraman Narayanan |
| author_sort | Jayavigneeswari Suresh babu |
| collection | DOAJ |
| description | Due to its numerous reported benefits, including biomimetic, immunomodulation, and compatibility with tissue microenvironment, natural bio composite nanomaterials are in demand as an effective replacement in the field of tissue engineering. As a result, in this study, we investigated polysaccharides containing natural materials from Coriandrum sativum(CS) and Trigonella foenum-graecum(TFG) to create a composite nanofiber matrix with polyvinyl alcohol (PVA). Drug-free and drug-containing versions of the natural composite nanofiber have been developed. Zeta potential, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy were used to characterize the conductivity, molecular makeup and size of the fibers (SEM). Brunauer-Emmett-Teller (BET) analysis was used to determine the topography of the surface. To determine how natural biomaterials will affect PVA, the stiffness of the composite nanofibers was tested. Drug release from the PVA composite nanofiber matrix using HPLC analysis was conducted on fibers loaded with drugs. Primary Human tenon fibroblast cells were used to test the fibers biocompatibility. SEM analysis revealed that bead-free composite nanofibers had homogeneous dimensions ranging from 150 nm to 200 nm. The presence of heterogeneous molecules from the natural material was revealed by FTIR data in the composite nanofiber. According to the BET analysis, the addition of natural biomaterial significantly altered the surface topography. With the addition of the drug 5-Fluorouracil, the stiffness of PVA nanofibers decreased from 103 ± 6 to 70 ± 15 Kpa. At the same time, the drug improved the mechanical properties of the composite nanofiber mats, increasing stiffness from 150 ± 22 Kpa to 180 ± 17 Kpa. Human Tenon fibroblast Cell toxicity assay showed that there was a difference in the cell viability of the composite nanofibers with the drug. The release kinetics of HPLC data showed that the composite matrix fibre mat had sustained drug release for 4 hrs. Cell viability assay findings revealed that composite nanofibers did not have any significant effect on the cell viability. Further research on the toxicity of the composite material in vivo is required to determine the mechanism. This may be helpful in modulating fibrosis process post trabeculectomy surgery. |
| first_indexed | 2024-04-10T17:23:36Z |
| format | Article |
| id | doaj.art-4f75c05a51ed4494851d7872af45fcec |
| institution | Directory Open Access Journal |
| issn | 2352-9520 |
| language | English |
| last_indexed | 2024-04-10T17:23:36Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | OpenNano |
| spelling | doaj.art-4f75c05a51ed4494851d7872af45fcec2023-02-05T04:16:33ZengElsevierOpenNano2352-95202023-03-0110100122Synthesis and characterization of natural biomaterial composite nanofibers for ocular drug delivery systemsJayavigneeswari Suresh babu0Aravindan Saravanan1Bharathselvi Muthuvel2Ronnie George3Janakiraman Narayanan4Department of Nanobiotechnology, Vision Research Foundation, Chennai, IndiaDepartment of Nanobiotechnology, Vision Research Foundation, Chennai, IndiaDepartment of Biochemistry, Vision Research Foundation, Chennai, IndiaDepartment of Glaucoma, Medical Research Foundation, Chennai, IndiaDepartment of Nanobiotechnology, Vision Research Foundation, Chennai, India; Corresponding author at: Department of Nanobiotechnology, KNBIRVO Block, Vision Research Foundation, Sankara Nethralaya campus, 18/41, College road Nungambakkam, Chennai, Tamil Nadu, 600006, India.Due to its numerous reported benefits, including biomimetic, immunomodulation, and compatibility with tissue microenvironment, natural bio composite nanomaterials are in demand as an effective replacement in the field of tissue engineering. As a result, in this study, we investigated polysaccharides containing natural materials from Coriandrum sativum(CS) and Trigonella foenum-graecum(TFG) to create a composite nanofiber matrix with polyvinyl alcohol (PVA). Drug-free and drug-containing versions of the natural composite nanofiber have been developed. Zeta potential, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy were used to characterize the conductivity, molecular makeup and size of the fibers (SEM). Brunauer-Emmett-Teller (BET) analysis was used to determine the topography of the surface. To determine how natural biomaterials will affect PVA, the stiffness of the composite nanofibers was tested. Drug release from the PVA composite nanofiber matrix using HPLC analysis was conducted on fibers loaded with drugs. Primary Human tenon fibroblast cells were used to test the fibers biocompatibility. SEM analysis revealed that bead-free composite nanofibers had homogeneous dimensions ranging from 150 nm to 200 nm. The presence of heterogeneous molecules from the natural material was revealed by FTIR data in the composite nanofiber. According to the BET analysis, the addition of natural biomaterial significantly altered the surface topography. With the addition of the drug 5-Fluorouracil, the stiffness of PVA nanofibers decreased from 103 ± 6 to 70 ± 15 Kpa. At the same time, the drug improved the mechanical properties of the composite nanofiber mats, increasing stiffness from 150 ± 22 Kpa to 180 ± 17 Kpa. Human Tenon fibroblast Cell toxicity assay showed that there was a difference in the cell viability of the composite nanofibers with the drug. The release kinetics of HPLC data showed that the composite matrix fibre mat had sustained drug release for 4 hrs. Cell viability assay findings revealed that composite nanofibers did not have any significant effect on the cell viability. Further research on the toxicity of the composite material in vivo is required to determine the mechanism. This may be helpful in modulating fibrosis process post trabeculectomy surgery.http://www.sciencedirect.com/science/article/pii/S2352952023000026Poly vinyl alcoholFenugreekCoriander5-FluorouracilHuman tenon fibroblastNanofiber |
| spellingShingle | Jayavigneeswari Suresh babu Aravindan Saravanan Bharathselvi Muthuvel Ronnie George Janakiraman Narayanan Synthesis and characterization of natural biomaterial composite nanofibers for ocular drug delivery systems OpenNano Poly vinyl alcohol Fenugreek Coriander 5-Fluorouracil Human tenon fibroblast Nanofiber |
| title | Synthesis and characterization of natural biomaterial composite nanofibers for ocular drug delivery systems |
| title_full | Synthesis and characterization of natural biomaterial composite nanofibers for ocular drug delivery systems |
| title_fullStr | Synthesis and characterization of natural biomaterial composite nanofibers for ocular drug delivery systems |
| title_full_unstemmed | Synthesis and characterization of natural biomaterial composite nanofibers for ocular drug delivery systems |
| title_short | Synthesis and characterization of natural biomaterial composite nanofibers for ocular drug delivery systems |
| title_sort | synthesis and characterization of natural biomaterial composite nanofibers for ocular drug delivery systems |
| topic | Poly vinyl alcohol Fenugreek Coriander 5-Fluorouracil Human tenon fibroblast Nanofiber |
| url | http://www.sciencedirect.com/science/article/pii/S2352952023000026 |
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