Wound Healing and Antioxidant Properties of <i>Launaea procumbens</i> Supported by Metabolomic Profiling and Molecular Docking
Wounds adversely affect people’s quality of life and have psychological, social, and economic impacts. Herbal remedies of <i>Launaea procumbens</i> (LP) are used to treat wounds. In an excision wound model, topical application of LP significantly promoted wound closure (on day 14, LP-tre...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/11/2258 |
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| author | Shaimaa R. Ahmed Ehab M. Mostafa Arafa Musa Enas Ezzat Rateb Mohammad M. Al-Sanea Dalia H. Abu-Baih Mahmoud A. Elrehany Entesar Ali Saber Mostafa E. Rateb Usama Ramadan Abdelmohsen |
| author_facet | Shaimaa R. Ahmed Ehab M. Mostafa Arafa Musa Enas Ezzat Rateb Mohammad M. Al-Sanea Dalia H. Abu-Baih Mahmoud A. Elrehany Entesar Ali Saber Mostafa E. Rateb Usama Ramadan Abdelmohsen |
| author_sort | Shaimaa R. Ahmed |
| collection | DOAJ |
| description | Wounds adversely affect people’s quality of life and have psychological, social, and economic impacts. Herbal remedies of <i>Launaea procumbens</i> (LP) are used to treat wounds. In an excision wound model, topical application of LP significantly promoted wound closure (on day 14, LP-treated animals had the highest percentages of wound closure in comparison with the other groups, as the wound was entirely closed with a closure percentage of 100%, <i>p</i> < 0.05). Histological analysis revealed a considerable rise in the number of fibroblasts, the amount of collagen, and its cross-linking in LP-treated wounds. Gene expression patterns showed significant elevation of TGF-β levels (2.1-fold change after 7 days treatment and 2.7-fold change in 14 days treatment) and downregulation of the inflammatory TNF-α and IL-1β levels in LP-treated wounds. Regarding in vitro antioxidant activity, LP extract significantly diminished the formation of H<sub>2</sub>O<sub>2</sub> radical (IC<sub>50</sub> = 171.6 μg/mL) and scavenged the superoxide radical (IC<sub>50</sub> of 286.7 µg/mL), indicating antioxidant potential in a dose-dependent manner. Dereplication of the secondary metabolites using LC-HRMS resulted in the annotation of 16 metabolites. The identified compounds were docked against important wound-healing targets, including vascular endothelial growth factor (VEGF), collagen α-1, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and transforming growth factor-β (TGF-β). Among dereplicated compounds, luteolin 8-C-glucoside (orientin) demonstrated binding potential to four investigated targets (VEGF, interleukin 1β, TNF-α, and collagen α-1). To conclude, <i>Launaea procumbens</i> extract could be regarded as a promising topical therapy to promote wound healing in excisional wounds, and luteolin 8-C-glucoside (orientin), one of its constituents, is a potential wound-healing drug lead. |
| first_indexed | 2024-03-09T18:31:08Z |
| format | Article |
| id | doaj.art-4f78f801237a42eaab3e23e4c2fb0124 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T18:31:08Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-4f78f801237a42eaab3e23e4c2fb01242023-11-24T07:32:19ZengMDPI AGAntioxidants2076-39212022-11-011111225810.3390/antiox11112258Wound Healing and Antioxidant Properties of <i>Launaea procumbens</i> Supported by Metabolomic Profiling and Molecular DockingShaimaa R. Ahmed0Ehab M. Mostafa1Arafa Musa2Enas Ezzat Rateb3Mohammad M. Al-Sanea4Dalia H. Abu-Baih5Mahmoud A. Elrehany6Entesar Ali Saber7Mostafa E. Rateb8Usama Ramadan Abdelmohsen9Department of Pharmacognosy, College of Pharmacy, Jouf University, Sakaka 72341, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, Jouf University, Sakaka 72341, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, Jouf University, Sakaka 72341, Saudi ArabiaDepartment of Physiology, Faculty of Medicine, Beni-Suef University, Beni-Suef 62521, EgyptPharmaceutical Chemistry Department, College of Pharmacy, Jouf University, Sakaka 72341, Saudi ArabiaDepartment of Biochemistry and Molecular Biology, Faculty of Pharmacy, Deraya University, New Minia 61111, EgyptDepartment of Biochemistry and Molecular Biology, Faculty of Pharmacy, Deraya University, New Minia 61111, EgyptDepartment of Medical Sciences (Histology), Deraya University, New Minia 61111, EgyptSchool of Computing, Engineering & Physical Sciences, University of the West of Scotland, Paisley PA1 2BE, UKDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, EgyptWounds adversely affect people’s quality of life and have psychological, social, and economic impacts. Herbal remedies of <i>Launaea procumbens</i> (LP) are used to treat wounds. In an excision wound model, topical application of LP significantly promoted wound closure (on day 14, LP-treated animals had the highest percentages of wound closure in comparison with the other groups, as the wound was entirely closed with a closure percentage of 100%, <i>p</i> < 0.05). Histological analysis revealed a considerable rise in the number of fibroblasts, the amount of collagen, and its cross-linking in LP-treated wounds. Gene expression patterns showed significant elevation of TGF-β levels (2.1-fold change after 7 days treatment and 2.7-fold change in 14 days treatment) and downregulation of the inflammatory TNF-α and IL-1β levels in LP-treated wounds. Regarding in vitro antioxidant activity, LP extract significantly diminished the formation of H<sub>2</sub>O<sub>2</sub> radical (IC<sub>50</sub> = 171.6 μg/mL) and scavenged the superoxide radical (IC<sub>50</sub> of 286.7 µg/mL), indicating antioxidant potential in a dose-dependent manner. Dereplication of the secondary metabolites using LC-HRMS resulted in the annotation of 16 metabolites. The identified compounds were docked against important wound-healing targets, including vascular endothelial growth factor (VEGF), collagen α-1, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and transforming growth factor-β (TGF-β). Among dereplicated compounds, luteolin 8-C-glucoside (orientin) demonstrated binding potential to four investigated targets (VEGF, interleukin 1β, TNF-α, and collagen α-1). To conclude, <i>Launaea procumbens</i> extract could be regarded as a promising topical therapy to promote wound healing in excisional wounds, and luteolin 8-C-glucoside (orientin), one of its constituents, is a potential wound-healing drug lead.https://www.mdpi.com/2076-3921/11/11/2258<i>Launaea procumbens</i>antioxidantwound healingLC-HRMS profilingdockingluteolin 8-C-glucoside |
| spellingShingle | Shaimaa R. Ahmed Ehab M. Mostafa Arafa Musa Enas Ezzat Rateb Mohammad M. Al-Sanea Dalia H. Abu-Baih Mahmoud A. Elrehany Entesar Ali Saber Mostafa E. Rateb Usama Ramadan Abdelmohsen Wound Healing and Antioxidant Properties of <i>Launaea procumbens</i> Supported by Metabolomic Profiling and Molecular Docking Antioxidants <i>Launaea procumbens</i> antioxidant wound healing LC-HRMS profiling docking luteolin 8-C-glucoside |
| title | Wound Healing and Antioxidant Properties of <i>Launaea procumbens</i> Supported by Metabolomic Profiling and Molecular Docking |
| title_full | Wound Healing and Antioxidant Properties of <i>Launaea procumbens</i> Supported by Metabolomic Profiling and Molecular Docking |
| title_fullStr | Wound Healing and Antioxidant Properties of <i>Launaea procumbens</i> Supported by Metabolomic Profiling and Molecular Docking |
| title_full_unstemmed | Wound Healing and Antioxidant Properties of <i>Launaea procumbens</i> Supported by Metabolomic Profiling and Molecular Docking |
| title_short | Wound Healing and Antioxidant Properties of <i>Launaea procumbens</i> Supported by Metabolomic Profiling and Molecular Docking |
| title_sort | wound healing and antioxidant properties of i launaea procumbens i supported by metabolomic profiling and molecular docking |
| topic | <i>Launaea procumbens</i> antioxidant wound healing LC-HRMS profiling docking luteolin 8-C-glucoside |
| url | https://www.mdpi.com/2076-3921/11/11/2258 |
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