Microwave- and Ultrasound-Assisted Extraction of Cucurbitane-Type Triterpenoids from <i>Momordica charantia</i> L. Cultivars and Their Antiproliferative Effect on SAS Human Oral Cancer Cells
Cucurbitane-type triterpenoids are a major class of bioactive compounds present in bitter melon. In the present study, six different cultivars of bitter melon were extracted by using microwave- or ultrasound-assisted techniques to identify the prominent method that can extract the majority of cucurb...
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MDPI AG
2022-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/5/729 |
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| author | Yu-Tsung Lee Li-Heng Pao Chi-Yuan Chen Sui-Qing Huang Alaganandam Kumaran Jong-Ho Chyuan Chun-Hui Chiu |
| author_facet | Yu-Tsung Lee Li-Heng Pao Chi-Yuan Chen Sui-Qing Huang Alaganandam Kumaran Jong-Ho Chyuan Chun-Hui Chiu |
| author_sort | Yu-Tsung Lee |
| collection | DOAJ |
| description | Cucurbitane-type triterpenoids are a major class of bioactive compounds present in bitter melon. In the present study, six different cultivars of bitter melon were extracted by using microwave- or ultrasound-assisted techniques to identify the prominent method that can extract the majority of cucurbitane-type triterpenoids. A UHPLC–MS/MS (ultra-high-performance liquid chromatography tandem mass spectrometry) system was used for the identification and quantification of ten cucurbitane-type triterpenoids. The results suggest that the use of microwave-assisted extraction on cultivars 4 and 5 produced higher amounts of the selected cucurbitane-type triterpenoids. The interpretation of principal component analysis also identified that cultivar 4 is significantly different from the others in which the compounds 3β,7β,25-trihydroxycucurbita-5,23(E)-dien-19-al and momordicine I were found in higher quantities. Upon further evaluation, it was also identified that these two triterpenoids can act as antiproliferative agents due to their effects on SAS human oral cancer cell lines. |
| first_indexed | 2024-03-09T20:39:45Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T20:39:45Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-4f79d9c498b940108f6ce0191f0542052023-11-23T23:01:34ZengMDPI AGFoods2304-81582022-03-0111572910.3390/foods11050729Microwave- and Ultrasound-Assisted Extraction of Cucurbitane-Type Triterpenoids from <i>Momordica charantia</i> L. Cultivars and Their Antiproliferative Effect on SAS Human Oral Cancer CellsYu-Tsung Lee0Li-Heng Pao1Chi-Yuan Chen2Sui-Qing Huang3Alaganandam Kumaran4Jong-Ho Chyuan5Chun-Hui Chiu6Graduate Institute of Health Industry and Technology, Research Center for Chinese Herbal Medicine and Research Center for Food and Cosmetic Safety, College of Human Ecology, Chang Gung University of Science and Technology, No. 261, Wenhua 1st Road, Guishan District, Taoyuan City 33303, TaiwanGraduate Institute of Health Industry and Technology, Research Center for Chinese Herbal Medicine and Research Center for Food and Cosmetic Safety, College of Human Ecology, Chang Gung University of Science and Technology, No. 261, Wenhua 1st Road, Guishan District, Taoyuan City 33303, TaiwanGraduate Institute of Health Industry and Technology, Research Center for Chinese Herbal Medicine and Research Center for Food and Cosmetic Safety, College of Human Ecology, Chang Gung University of Science and Technology, No. 261, Wenhua 1st Road, Guishan District, Taoyuan City 33303, TaiwanGraduate Institute of Health Industry and Technology, Research Center for Chinese Herbal Medicine and Research Center for Food and Cosmetic Safety, College of Human Ecology, Chang Gung University of Science and Technology, No. 261, Wenhua 1st Road, Guishan District, Taoyuan City 33303, TaiwanChemical Sciences and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Thiruvananthapuram, Kerala City 695019, IndiaHualien District Agricultural Research and Extension Station, Hualien City 973044, TaiwanGraduate Institute of Health Industry and Technology, Research Center for Chinese Herbal Medicine and Research Center for Food and Cosmetic Safety, College of Human Ecology, Chang Gung University of Science and Technology, No. 261, Wenhua 1st Road, Guishan District, Taoyuan City 33303, TaiwanCucurbitane-type triterpenoids are a major class of bioactive compounds present in bitter melon. In the present study, six different cultivars of bitter melon were extracted by using microwave- or ultrasound-assisted techniques to identify the prominent method that can extract the majority of cucurbitane-type triterpenoids. A UHPLC–MS/MS (ultra-high-performance liquid chromatography tandem mass spectrometry) system was used for the identification and quantification of ten cucurbitane-type triterpenoids. The results suggest that the use of microwave-assisted extraction on cultivars 4 and 5 produced higher amounts of the selected cucurbitane-type triterpenoids. The interpretation of principal component analysis also identified that cultivar 4 is significantly different from the others in which the compounds 3β,7β,25-trihydroxycucurbita-5,23(E)-dien-19-al and momordicine I were found in higher quantities. Upon further evaluation, it was also identified that these two triterpenoids can act as antiproliferative agents due to their effects on SAS human oral cancer cell lines.https://www.mdpi.com/2304-8158/11/5/729<i>Momordica charantia</i> L.microwave-assisted extraction3β,7β,25-trihydroxycucurbita-5,23(E)-dien-19-almomordicine IUHPLC–MS/MSSAS cell lines |
| spellingShingle | Yu-Tsung Lee Li-Heng Pao Chi-Yuan Chen Sui-Qing Huang Alaganandam Kumaran Jong-Ho Chyuan Chun-Hui Chiu Microwave- and Ultrasound-Assisted Extraction of Cucurbitane-Type Triterpenoids from <i>Momordica charantia</i> L. Cultivars and Their Antiproliferative Effect on SAS Human Oral Cancer Cells Foods <i>Momordica charantia</i> L. microwave-assisted extraction 3β,7β,25-trihydroxycucurbita-5,23(E)-dien-19-al momordicine I UHPLC–MS/MS SAS cell lines |
| title | Microwave- and Ultrasound-Assisted Extraction of Cucurbitane-Type Triterpenoids from <i>Momordica charantia</i> L. Cultivars and Their Antiproliferative Effect on SAS Human Oral Cancer Cells |
| title_full | Microwave- and Ultrasound-Assisted Extraction of Cucurbitane-Type Triterpenoids from <i>Momordica charantia</i> L. Cultivars and Their Antiproliferative Effect on SAS Human Oral Cancer Cells |
| title_fullStr | Microwave- and Ultrasound-Assisted Extraction of Cucurbitane-Type Triterpenoids from <i>Momordica charantia</i> L. Cultivars and Their Antiproliferative Effect on SAS Human Oral Cancer Cells |
| title_full_unstemmed | Microwave- and Ultrasound-Assisted Extraction of Cucurbitane-Type Triterpenoids from <i>Momordica charantia</i> L. Cultivars and Their Antiproliferative Effect on SAS Human Oral Cancer Cells |
| title_short | Microwave- and Ultrasound-Assisted Extraction of Cucurbitane-Type Triterpenoids from <i>Momordica charantia</i> L. Cultivars and Their Antiproliferative Effect on SAS Human Oral Cancer Cells |
| title_sort | microwave and ultrasound assisted extraction of cucurbitane type triterpenoids from i momordica charantia i l cultivars and their antiproliferative effect on sas human oral cancer cells |
| topic | <i>Momordica charantia</i> L. microwave-assisted extraction 3β,7β,25-trihydroxycucurbita-5,23(E)-dien-19-al momordicine I UHPLC–MS/MS SAS cell lines |
| url | https://www.mdpi.com/2304-8158/11/5/729 |
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