Identification and characterization of extrachromosomal circular DNA in patients with high myopia and cataract
To explore the presence of extrachromosomal circular DNA (eccDNA) in the anterior capsule of the lens in the eyes of patients with cataract and with high myopia. Circle-Seq was performed to identify differences in the eccDNA and gene expression between the anterior capsule of the lens of patients wi...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Epigenetics |
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| Online Access: | http://dx.doi.org/10.1080/15592294.2023.2192324 |
| _version_ | 1797678813986947072 |
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| author | Kai Wen Lujie Zhang Yang Cai He Teng Jingli Liang Yi Yue Yaoling Li Yifang Huang Ming Liu Yufeng Zhang Ruihua Wei Jing Sun |
| author_facet | Kai Wen Lujie Zhang Yang Cai He Teng Jingli Liang Yi Yue Yaoling Li Yifang Huang Ming Liu Yufeng Zhang Ruihua Wei Jing Sun |
| author_sort | Kai Wen |
| collection | DOAJ |
| description | To explore the presence of extrachromosomal circular DNA (eccDNA) in the anterior capsule of the lens in the eyes of patients with cataract and with high myopia. Circle-Seq was performed to identify differences in the eccDNA and gene expression between the anterior capsule of the lens of patients with simple nuclear cataract (C, n = 6 cases) and patients with nuclear cataract along with high myopia (HM, n = 6 cases). The expression of eccDNA was confirmed using routine quantitative polymerase chain reaction. The eccDNA ranked in C and HM ranged in length from 0.017 kb − 9.9 Mb with two distinctive peaks detected at 0.2 kb and 0.5 kb, while eccDNA that were differentially expressed ranged in size from 0.05 kb − 57.8 kb with two distinctive peaks observed at 0.1 kb and 0.5 kb. Only 2.5% of the eccDNA in C and 2% in HM were>25 kb in size. The gene-rich chromosomes contributed to more number of eccDNA/Mb, while several well-known high myopia candidate genes, including catenin delta 2 (CTNND2) and ubiquitin-like with PHD, exhibited significantly increased levels of eccDNA in the anterior capsule of the lens in patients with high myopia. This study highlighted the topologic analysis of the anterior capsule of eyes with high myopia, which is an emerging direction for research and clinical applications. These findings suggested that eccDNA was commonly detected in eyes with high myopia and cataracts, and the candidate genes for high myopia identified in previous studies were also observed in the eccDNA. |
| first_indexed | 2024-03-11T23:05:23Z |
| format | Article |
| id | doaj.art-4f7a28961f71437b864539bbe90cdd60 |
| institution | Directory Open Access Journal |
| issn | 1559-2294 1559-2308 |
| language | English |
| last_indexed | 2024-03-11T23:05:23Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj.art-4f7a28961f71437b864539bbe90cdd602023-09-21T13:23:13ZengTaylor & Francis GroupEpigenetics1559-22941559-23082023-12-0118110.1080/15592294.2023.21923242192324Identification and characterization of extrachromosomal circular DNA in patients with high myopia and cataractKai Wen0Lujie Zhang1Yang Cai2He Teng3Jingli Liang4Yi Yue5Yaoling Li6Yifang Huang7Ming Liu8Yufeng Zhang9Ruihua Wei10Jing Sun11Tianjin Medical University Eye HospitalTianjin Children’s HospitalTianjin Medical University Eye HospitalTianjin Medical University Eye HospitalTianjin Medical University Eye HospitalTianjin Medical University Eye HospitalTianjin Medical University Eye HospitalTianjin Medical University Eye HospitalTianjin Medical University Eye HospitalTianjin Medical University Eye HospitalTianjin Medical University Eye HospitalTianjin Medical University Eye HospitalTo explore the presence of extrachromosomal circular DNA (eccDNA) in the anterior capsule of the lens in the eyes of patients with cataract and with high myopia. Circle-Seq was performed to identify differences in the eccDNA and gene expression between the anterior capsule of the lens of patients with simple nuclear cataract (C, n = 6 cases) and patients with nuclear cataract along with high myopia (HM, n = 6 cases). The expression of eccDNA was confirmed using routine quantitative polymerase chain reaction. The eccDNA ranked in C and HM ranged in length from 0.017 kb − 9.9 Mb with two distinctive peaks detected at 0.2 kb and 0.5 kb, while eccDNA that were differentially expressed ranged in size from 0.05 kb − 57.8 kb with two distinctive peaks observed at 0.1 kb and 0.5 kb. Only 2.5% of the eccDNA in C and 2% in HM were>25 kb in size. The gene-rich chromosomes contributed to more number of eccDNA/Mb, while several well-known high myopia candidate genes, including catenin delta 2 (CTNND2) and ubiquitin-like with PHD, exhibited significantly increased levels of eccDNA in the anterior capsule of the lens in patients with high myopia. This study highlighted the topologic analysis of the anterior capsule of eyes with high myopia, which is an emerging direction for research and clinical applications. These findings suggested that eccDNA was commonly detected in eyes with high myopia and cataracts, and the candidate genes for high myopia identified in previous studies were also observed in the eccDNA.http://dx.doi.org/10.1080/15592294.2023.2192324extrachromosomal circular dnahigh myopiacataractcircle-seqanterior capsule of the lens |
| spellingShingle | Kai Wen Lujie Zhang Yang Cai He Teng Jingli Liang Yi Yue Yaoling Li Yifang Huang Ming Liu Yufeng Zhang Ruihua Wei Jing Sun Identification and characterization of extrachromosomal circular DNA in patients with high myopia and cataract Epigenetics extrachromosomal circular dna high myopia cataract circle-seq anterior capsule of the lens |
| title | Identification and characterization of extrachromosomal circular DNA in patients with high myopia and cataract |
| title_full | Identification and characterization of extrachromosomal circular DNA in patients with high myopia and cataract |
| title_fullStr | Identification and characterization of extrachromosomal circular DNA in patients with high myopia and cataract |
| title_full_unstemmed | Identification and characterization of extrachromosomal circular DNA in patients with high myopia and cataract |
| title_short | Identification and characterization of extrachromosomal circular DNA in patients with high myopia and cataract |
| title_sort | identification and characterization of extrachromosomal circular dna in patients with high myopia and cataract |
| topic | extrachromosomal circular dna high myopia cataract circle-seq anterior capsule of the lens |
| url | http://dx.doi.org/10.1080/15592294.2023.2192324 |
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