Fine Mapping of Two Interacting Loci for Transmission Ratio Distortion in Rice (Oryza sativa L.)
Transmission ratio distortion (TRD) denotes the observed allelic or genotypic frequency deviation from the expected Mendelian segregation ratios in the offspring of a heterozygote. TRD can severely hamper gene flow between and within rice species. Here, we report the fine mapping and characterizatio...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.866276/full |
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| author | Chaopu Zhang Chaopu Zhang Jilin Wang Jilin Wang Xiongfeng Xiao Xiongfeng Xiao Dianwen Wang Dianwen Wang Zhiyang Yuan Zhiyang Yuan Xiaodan Zhang Xiaodan Zhang Wenqiang Sun Wenqiang Sun Sibin Yu Sibin Yu Sibin Yu |
| author_facet | Chaopu Zhang Chaopu Zhang Jilin Wang Jilin Wang Xiongfeng Xiao Xiongfeng Xiao Dianwen Wang Dianwen Wang Zhiyang Yuan Zhiyang Yuan Xiaodan Zhang Xiaodan Zhang Wenqiang Sun Wenqiang Sun Sibin Yu Sibin Yu Sibin Yu |
| author_sort | Chaopu Zhang |
| collection | DOAJ |
| description | Transmission ratio distortion (TRD) denotes the observed allelic or genotypic frequency deviation from the expected Mendelian segregation ratios in the offspring of a heterozygote. TRD can severely hamper gene flow between and within rice species. Here, we report the fine mapping and characterization of two loci (TRD4.1 and TRD4.2) for TRD using large F2 segregating populations, which are derived from rice chromosome segment substitution lines, each containing a particular genomic segment introduced from the japonica cultivar Nipponbare (NIP) into the indica cultivar Zhenshan (ZS97). The two loci exhibited a preferential transmission of ZS97 alleles in the derived progeny. Reciprocal crossing experiments using near-isogenic lines harboring three different alleles at TRD4.1 suggest that the gene causes male gametic selection. Moreover, the transmission bias of TRD4.2 was diminished in heterozygotes when they carried homozygous TRD4.1ZS97. This indicates an epistatic interaction between these two loci. TRD4.2 was mapped into a 35-kb region encompassing one candidate gene that is specifically expressed in the reproductive organs in rice. These findings broaden the understanding of the genetic mechanisms of TRD and offer an approach to overcome the barrier of gene flow between the subspecies in rice, thus facilitating rice improvement by introgression breeding. |
| first_indexed | 2024-12-18T05:41:03Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-18T05:41:03Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-4f7aa590327644a099e01628b80e99c82022-12-21T21:19:11ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-03-011310.3389/fpls.2022.866276866276Fine Mapping of Two Interacting Loci for Transmission Ratio Distortion in Rice (Oryza sativa L.)Chaopu Zhang0Chaopu Zhang1Jilin Wang2Jilin Wang3Xiongfeng Xiao4Xiongfeng Xiao5Dianwen Wang6Dianwen Wang7Zhiyang Yuan8Zhiyang Yuan9Xiaodan Zhang10Xiaodan Zhang11Wenqiang Sun12Wenqiang Sun13Sibin Yu14Sibin Yu15Sibin Yu16National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaHubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, ChinaTransmission ratio distortion (TRD) denotes the observed allelic or genotypic frequency deviation from the expected Mendelian segregation ratios in the offspring of a heterozygote. TRD can severely hamper gene flow between and within rice species. Here, we report the fine mapping and characterization of two loci (TRD4.1 and TRD4.2) for TRD using large F2 segregating populations, which are derived from rice chromosome segment substitution lines, each containing a particular genomic segment introduced from the japonica cultivar Nipponbare (NIP) into the indica cultivar Zhenshan (ZS97). The two loci exhibited a preferential transmission of ZS97 alleles in the derived progeny. Reciprocal crossing experiments using near-isogenic lines harboring three different alleles at TRD4.1 suggest that the gene causes male gametic selection. Moreover, the transmission bias of TRD4.2 was diminished in heterozygotes when they carried homozygous TRD4.1ZS97. This indicates an epistatic interaction between these two loci. TRD4.2 was mapped into a 35-kb region encompassing one candidate gene that is specifically expressed in the reproductive organs in rice. These findings broaden the understanding of the genetic mechanisms of TRD and offer an approach to overcome the barrier of gene flow between the subspecies in rice, thus facilitating rice improvement by introgression breeding.https://www.frontiersin.org/articles/10.3389/fpls.2022.866276/fullricereproductive isolationtransmission ratio distortionallele frequencygametic selectionepistatic interaction |
| spellingShingle | Chaopu Zhang Chaopu Zhang Jilin Wang Jilin Wang Xiongfeng Xiao Xiongfeng Xiao Dianwen Wang Dianwen Wang Zhiyang Yuan Zhiyang Yuan Xiaodan Zhang Xiaodan Zhang Wenqiang Sun Wenqiang Sun Sibin Yu Sibin Yu Sibin Yu Fine Mapping of Two Interacting Loci for Transmission Ratio Distortion in Rice (Oryza sativa L.) Frontiers in Plant Science rice reproductive isolation transmission ratio distortion allele frequency gametic selection epistatic interaction |
| title | Fine Mapping of Two Interacting Loci for Transmission Ratio Distortion in Rice (Oryza sativa L.) |
| title_full | Fine Mapping of Two Interacting Loci for Transmission Ratio Distortion in Rice (Oryza sativa L.) |
| title_fullStr | Fine Mapping of Two Interacting Loci for Transmission Ratio Distortion in Rice (Oryza sativa L.) |
| title_full_unstemmed | Fine Mapping of Two Interacting Loci for Transmission Ratio Distortion in Rice (Oryza sativa L.) |
| title_short | Fine Mapping of Two Interacting Loci for Transmission Ratio Distortion in Rice (Oryza sativa L.) |
| title_sort | fine mapping of two interacting loci for transmission ratio distortion in rice oryza sativa l |
| topic | rice reproductive isolation transmission ratio distortion allele frequency gametic selection epistatic interaction |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.866276/full |
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