Blood transcriptome analysis revealing aging gene expression profiles in red panda
The red panda is an endangered forest species distributed on the edge of the Qinghai Tibet Plateau. The species has been conserved in ex-situ in many countries and its survival is threatened by many diseases. Its immune system is vulnerable to age-associated alterations, which accumulate and result...
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PeerJ Inc.
2022-07-01
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| Online Access: | https://peerj.com/articles/13743.pdf |
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| author | Jing Luo Liang Zhang Fujun Shen Li Luo Lei Chen Zhenxin Fan Rong Hou Bisong Yue Xiuyue Zhang |
| author_facet | Jing Luo Liang Zhang Fujun Shen Li Luo Lei Chen Zhenxin Fan Rong Hou Bisong Yue Xiuyue Zhang |
| author_sort | Jing Luo |
| collection | DOAJ |
| description | The red panda is an endangered forest species distributed on the edge of the Qinghai Tibet Plateau. The species has been conserved in ex-situ in many countries and its survival is threatened by many diseases. Its immune system is vulnerable to age-associated alterations, which accumulate and result in a progressive deterioration that leads to an increased incidence of diseases. We identified 2,219 differentially expressed genes (DEGs) between geriatric (11–16 years) and adult individuals (4–8 years), and 1690 DEGs between adults and juveniles (1 year). The gene expression and functional annotation results showed that the innate immunity of red pandas increases significantly in geriatric individuals, whereas its change remains unclear when comparing adults and juveniles. We found that the adaptive immunity of red pandas first increased and then decreased with age. We identified CXCR3, BLNK, and CCR4 as the hub genes in the age-related protein–protein interaction network, which showed their central role in age-related immune changes. Many DNA repair genes were down-regulated in geriatric red pandas, suggesting that the DNA repair ability of the blood tissue in geriatric red pandas is significantly reduced. The significantly up-regulated TLR5 in geriatric individuals also suggests the possibility of enhancing the vaccination immune response by incorporating flagellin, which could be used to address decreased vaccine responses caused by age-related declines in immune system function. This work provides an insight into gene expression changes associated with aging and paves the way for effective disease prevention and treatment strategies for red pandas in the future. |
| first_indexed | 2024-03-09T08:18:27Z |
| format | Article |
| id | doaj.art-4f7ac3287cb44615bcaffefdcf93d625 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:18:27Z |
| publishDate | 2022-07-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-4f7ac3287cb44615bcaffefdcf93d6252023-12-02T21:55:52ZengPeerJ Inc.PeerJ2167-83592022-07-0110e1374310.7717/peerj.13743Blood transcriptome analysis revealing aging gene expression profiles in red pandaJing Luo0Liang Zhang1Fujun Shen2Li Luo3Lei Chen4Zhenxin Fan5Rong Hou6Bisong Yue7Xiuyue Zhang8Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu, ChinaSichuan Key Laboratory for Conservation Biology of Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Sichuan, ChinaSichuan Key Laboratory for Conservation Biology of Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Sichuan, ChinaSichuan Key Laboratory for Conservation Biology of Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Sichuan, ChinaKey Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu, ChinaKey Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu, ChinaSichuan Key Laboratory for Conservation Biology of Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Sichuan, ChinaKey Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu, ChinaKey Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu, ChinaThe red panda is an endangered forest species distributed on the edge of the Qinghai Tibet Plateau. The species has been conserved in ex-situ in many countries and its survival is threatened by many diseases. Its immune system is vulnerable to age-associated alterations, which accumulate and result in a progressive deterioration that leads to an increased incidence of diseases. We identified 2,219 differentially expressed genes (DEGs) between geriatric (11–16 years) and adult individuals (4–8 years), and 1690 DEGs between adults and juveniles (1 year). The gene expression and functional annotation results showed that the innate immunity of red pandas increases significantly in geriatric individuals, whereas its change remains unclear when comparing adults and juveniles. We found that the adaptive immunity of red pandas first increased and then decreased with age. We identified CXCR3, BLNK, and CCR4 as the hub genes in the age-related protein–protein interaction network, which showed their central role in age-related immune changes. Many DNA repair genes were down-regulated in geriatric red pandas, suggesting that the DNA repair ability of the blood tissue in geriatric red pandas is significantly reduced. The significantly up-regulated TLR5 in geriatric individuals also suggests the possibility of enhancing the vaccination immune response by incorporating flagellin, which could be used to address decreased vaccine responses caused by age-related declines in immune system function. This work provides an insight into gene expression changes associated with aging and paves the way for effective disease prevention and treatment strategies for red pandas in the future.https://peerj.com/articles/13743.pdfRed pandaTranscriptomeAgingImmune alternationsDisease prevention |
| spellingShingle | Jing Luo Liang Zhang Fujun Shen Li Luo Lei Chen Zhenxin Fan Rong Hou Bisong Yue Xiuyue Zhang Blood transcriptome analysis revealing aging gene expression profiles in red panda PeerJ Red panda Transcriptome Aging Immune alternations Disease prevention |
| title | Blood transcriptome analysis revealing aging gene expression profiles in red panda |
| title_full | Blood transcriptome analysis revealing aging gene expression profiles in red panda |
| title_fullStr | Blood transcriptome analysis revealing aging gene expression profiles in red panda |
| title_full_unstemmed | Blood transcriptome analysis revealing aging gene expression profiles in red panda |
| title_short | Blood transcriptome analysis revealing aging gene expression profiles in red panda |
| title_sort | blood transcriptome analysis revealing aging gene expression profiles in red panda |
| topic | Red panda Transcriptome Aging Immune alternations Disease prevention |
| url | https://peerj.com/articles/13743.pdf |
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