Correlation analysis between peripheral blood dendritic cell subsets and PD-1 in patients with peritoneal adenocarcinoma
Abstract The aim of this study was to explore the association between differential percentages of dendritic cell (DC) subsets in peripheral blood and malignancy (grade and lymph node metastasis) of peritoneal adenocarcinoma patients and the frequencies of dendritic cell subsets in the normal control...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2024-02-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2024000100614&lng=en&tlng=en |
| _version_ | 1827346693987762176 |
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| author | Huihui Hu Man Zhang |
| author_facet | Huihui Hu Man Zhang |
| author_sort | Huihui Hu |
| collection | DOAJ |
| description | Abstract The aim of this study was to explore the association between differential percentages of dendritic cell (DC) subsets in peripheral blood and malignancy (grade and lymph node metastasis) of peritoneal adenocarcinoma patients and the frequencies of dendritic cell subsets in the normal controls. The peripheral blood of 30 patients with peritoneal adenocarcinoma and 12 healthy controls were collected for multicolor flow cytometry analysis. Peritoneal adenocarcinoma patients were grouped according to the malignant degree (grade and lymph node metastasis). Percentages of myeloid DCs (mDCs) and its subsets MDC1 and MDC2 in DCs were lower in peripheral blood of patients with peritoneal adenocarcinoma than in normal controls. The percentages of plasmacytoid dendritic cells (pDCs) and CD16+mDCs in DCs were higher than in normal controls. Compared with poor differentiation grade, patients with well/moderate differentiation grade had an increased percentage of CD16+mDCs. Contrary to CD16+mDCs, the percentage of MDC1 was lower in the well/moderate differentiation grade group. In patients with no lymph node metastasis, pDCs and CD16+mDCs levels were higher compared with patients with lymph node metastasis. mDCs and MDC1 levels had opposite results. pDCs were positively correlated with CD16+mDCs in peripheral blood of peritoneal patients, as was mDCs and MDC1. CD16+mDCs were negatively correlated with MDC1. The percentages of pDCs and CD16+mDCs in DCs were positively correlated with CD3+CD8+T cells, and pDCs also positively correlated with CD8+PD-1+T cells. Our results revealed that DCs subsets correlated with peritoneal adenocarcinoma malignancy. Dendritic cells play an independent role in the immune function of peritoneal adenocarcinoma. |
| first_indexed | 2024-03-07T23:35:21Z |
| format | Article |
| id | doaj.art-4f7af36d4cc846899fe8a1930bba14fa |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-03-07T23:35:21Z |
| publishDate | 2024-02-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-4f7af36d4cc846899fe8a1930bba14fa2024-02-20T07:40:45ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2024-02-015710.1590/1414-431x2023e13192Correlation analysis between peripheral blood dendritic cell subsets and PD-1 in patients with peritoneal adenocarcinomaHuihui Huhttps://orcid.org/0000-0002-9418-2639Man Zhanghttps://orcid.org/0000-0001-5166-6804Abstract The aim of this study was to explore the association between differential percentages of dendritic cell (DC) subsets in peripheral blood and malignancy (grade and lymph node metastasis) of peritoneal adenocarcinoma patients and the frequencies of dendritic cell subsets in the normal controls. The peripheral blood of 30 patients with peritoneal adenocarcinoma and 12 healthy controls were collected for multicolor flow cytometry analysis. Peritoneal adenocarcinoma patients were grouped according to the malignant degree (grade and lymph node metastasis). Percentages of myeloid DCs (mDCs) and its subsets MDC1 and MDC2 in DCs were lower in peripheral blood of patients with peritoneal adenocarcinoma than in normal controls. The percentages of plasmacytoid dendritic cells (pDCs) and CD16+mDCs in DCs were higher than in normal controls. Compared with poor differentiation grade, patients with well/moderate differentiation grade had an increased percentage of CD16+mDCs. Contrary to CD16+mDCs, the percentage of MDC1 was lower in the well/moderate differentiation grade group. In patients with no lymph node metastasis, pDCs and CD16+mDCs levels were higher compared with patients with lymph node metastasis. mDCs and MDC1 levels had opposite results. pDCs were positively correlated with CD16+mDCs in peripheral blood of peritoneal patients, as was mDCs and MDC1. CD16+mDCs were negatively correlated with MDC1. The percentages of pDCs and CD16+mDCs in DCs were positively correlated with CD3+CD8+T cells, and pDCs also positively correlated with CD8+PD-1+T cells. Our results revealed that DCs subsets correlated with peritoneal adenocarcinoma malignancy. Dendritic cells play an independent role in the immune function of peritoneal adenocarcinoma.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2024000100614&lng=en&tlng=enDendritic cellsPD-1Peripheral bloodPeritoneal adenocarcinomaFlow cytometry |
| spellingShingle | Huihui Hu Man Zhang Correlation analysis between peripheral blood dendritic cell subsets and PD-1 in patients with peritoneal adenocarcinoma Brazilian Journal of Medical and Biological Research Dendritic cells PD-1 Peripheral blood Peritoneal adenocarcinoma Flow cytometry |
| title | Correlation analysis between peripheral blood dendritic cell subsets and PD-1 in patients with peritoneal adenocarcinoma |
| title_full | Correlation analysis between peripheral blood dendritic cell subsets and PD-1 in patients with peritoneal adenocarcinoma |
| title_fullStr | Correlation analysis between peripheral blood dendritic cell subsets and PD-1 in patients with peritoneal adenocarcinoma |
| title_full_unstemmed | Correlation analysis between peripheral blood dendritic cell subsets and PD-1 in patients with peritoneal adenocarcinoma |
| title_short | Correlation analysis between peripheral blood dendritic cell subsets and PD-1 in patients with peritoneal adenocarcinoma |
| title_sort | correlation analysis between peripheral blood dendritic cell subsets and pd 1 in patients with peritoneal adenocarcinoma |
| topic | Dendritic cells PD-1 Peripheral blood Peritoneal adenocarcinoma Flow cytometry |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2024000100614&lng=en&tlng=en |
| work_keys_str_mv | AT huihuihu correlationanalysisbetweenperipheralblooddendriticcellsubsetsandpd1inpatientswithperitonealadenocarcinoma AT manzhang correlationanalysisbetweenperipheralblooddendriticcellsubsetsandpd1inpatientswithperitonealadenocarcinoma |