KIAA1429 facilitates progression of hepatocellular carcinoma by modulating m6A levels in HPN
Background: Most N6-methyladenosine (m6A)-associated modulatory proteins are involved in the pathogenesis of various cancers. The roles of m6A-related genes in liver hepatocellular carcinoma (LIHC) and the associated mechanisms remain unknown. Methods: GEO and GEPIA2 databases were used to identify...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023092927 |
| _version_ | 1797429685713371136 |
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| author | Yu Meng Wenwen Yang Jinchao Li Wei Chai |
| author_facet | Yu Meng Wenwen Yang Jinchao Li Wei Chai |
| author_sort | Yu Meng |
| collection | DOAJ |
| description | Background: Most N6-methyladenosine (m6A)-associated modulatory proteins are involved in the pathogenesis of various cancers. The roles of m6A-related genes in liver hepatocellular carcinoma (LIHC) and the associated mechanisms remain unknown. Methods: GEO and GEPIA2 databases were used to identify the m6A modification-related genes which were differentially expressed in LIHC and adjacent non-tumor tissues, and quantitative PCR was used to evaluate the expression of KIAA1429, a major m6A methyltransferase, in LIHC cells. The effect of KIAA1429 on the malignant phenotypes of LIHC cells was evaluated in vitro. The UALCAN, GEPIA, and GEO databases and western blotting assays were used to identify the target genes of KIAA1429. Results: KIAA1429 expression was markedly elevated in LIHC tissues, and patients with LIHC who had high KIAA1429 expression had a worse prognosis than those who had low expression. KIAA1429 silencing attenuated LIHC metastasis and proliferation. KIAA142 regulates m6A levels in HPN to intensify LIHC progression. Conclusion: Our study suggests a KIAA1429-HPN modulatory model based on m6A modifications, that offers insights into the occurrence and development of LIHC. |
| first_indexed | 2024-03-09T09:16:44Z |
| format | Article |
| id | doaj.art-4f7bd46b7f9f4cd98ed27992ada669c9 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-09T09:16:44Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-4f7bd46b7f9f4cd98ed27992ada669c92023-12-02T07:05:08ZengElsevierHeliyon2405-84402023-11-01911e22084KIAA1429 facilitates progression of hepatocellular carcinoma by modulating m6A levels in HPNYu Meng0Wenwen Yang1Jinchao Li2Wei Chai3The First Department of Hepatobiliary and Pancreatic Surgery, Cangzhou Central Hospital, Cangzhou, ChinaThe Department of Nursing, Cangzhou Medical College, Cangzhou, ChinaThe First Department of Hepatobiliary and Pancreatic Surgery, Cangzhou Central Hospital, Cangzhou, ChinaThe First Department of Hepatobiliary and Pancreatic Surgery, Cangzhou Central Hospital, Cangzhou, China; Corresponding author. No. 16 Xinhua West Road, Yunhe District, Cangzhou, Hebei, China.Background: Most N6-methyladenosine (m6A)-associated modulatory proteins are involved in the pathogenesis of various cancers. The roles of m6A-related genes in liver hepatocellular carcinoma (LIHC) and the associated mechanisms remain unknown. Methods: GEO and GEPIA2 databases were used to identify the m6A modification-related genes which were differentially expressed in LIHC and adjacent non-tumor tissues, and quantitative PCR was used to evaluate the expression of KIAA1429, a major m6A methyltransferase, in LIHC cells. The effect of KIAA1429 on the malignant phenotypes of LIHC cells was evaluated in vitro. The UALCAN, GEPIA, and GEO databases and western blotting assays were used to identify the target genes of KIAA1429. Results: KIAA1429 expression was markedly elevated in LIHC tissues, and patients with LIHC who had high KIAA1429 expression had a worse prognosis than those who had low expression. KIAA1429 silencing attenuated LIHC metastasis and proliferation. KIAA142 regulates m6A levels in HPN to intensify LIHC progression. Conclusion: Our study suggests a KIAA1429-HPN modulatory model based on m6A modifications, that offers insights into the occurrence and development of LIHC.http://www.sciencedirect.com/science/article/pii/S2405844023092927KIAA1429Hepatocellular carcinomam6A methylationHPNMigration |
| spellingShingle | Yu Meng Wenwen Yang Jinchao Li Wei Chai KIAA1429 facilitates progression of hepatocellular carcinoma by modulating m6A levels in HPN Heliyon KIAA1429 Hepatocellular carcinoma m6A methylation HPN Migration |
| title | KIAA1429 facilitates progression of hepatocellular carcinoma by modulating m6A levels in HPN |
| title_full | KIAA1429 facilitates progression of hepatocellular carcinoma by modulating m6A levels in HPN |
| title_fullStr | KIAA1429 facilitates progression of hepatocellular carcinoma by modulating m6A levels in HPN |
| title_full_unstemmed | KIAA1429 facilitates progression of hepatocellular carcinoma by modulating m6A levels in HPN |
| title_short | KIAA1429 facilitates progression of hepatocellular carcinoma by modulating m6A levels in HPN |
| title_sort | kiaa1429 facilitates progression of hepatocellular carcinoma by modulating m6a levels in hpn |
| topic | KIAA1429 Hepatocellular carcinoma m6A methylation HPN Migration |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023092927 |
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