Study of the association between the polymorphism of the TNF-α gene and prostate cancer
Background: Prostate cancer is the third cause of cancer death in men in the Western hemisphere and the second cause of cancer death in Zulian men from Venezuela. Objective: To determine whether polymorphisms 308 and 238 of the tumor necrosis factor alpha (TNF-α) gene are associated with prostate c...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Colegio Mexicano de Inmunología Clínica y Alergia, A.C.
2019-06-01
|
| Series: | Revista Alergia México |
| Subjects: | |
| Online Access: | http://revistaalergia.mx/ojs/index.php/ram/article/view/517 |
| _version_ | 1797713345180073984 |
|---|---|
| author | Tatiana Pardo Pedro Salcedo José Miguel Quintero Lisbeth Borjas Mercedes Fernández-Mestre Yanira Sánchez Zuhey Carrillo Sergio Rivera |
| author_facet | Tatiana Pardo Pedro Salcedo José Miguel Quintero Lisbeth Borjas Mercedes Fernández-Mestre Yanira Sánchez Zuhey Carrillo Sergio Rivera |
| author_sort | Tatiana Pardo |
| collection | DOAJ |
| description | Background: Prostate cancer is the third cause of cancer death in men in the Western hemisphere and the second cause of cancer death in Zulian men from Venezuela.
Objective: To determine whether polymorphisms 308 and 238 of the tumor necrosis factor alpha (TNF-α) gene are associated with prostate cancer.
Methods: The DNA that was extracted from the peripheral blood of 40 patients with prostatic specific antigen and 40 controls was amplified by PCR plus digestion with enzymes NcoI and MspI.
Results: In the patients, genotypes of the TNF-α-238 polymorphism were observed in 90% GG and 10% GA; in controls, in 97.5% GG and 2.5% GA, odds ratio (OR) = 4,000 for GA. In the patients, genotypes of TNF-α-308 polymorphism were identified in 85% GG and 15% GA, and in controls in 72.5% GG and 27.5% GA, OR = 0.545 for GA and 1.172 for GG. The allelic frequencies for TNF-α-238 in patients were 95% for G and 5% for A; in controls, 98.75% for G and 1.25% for A, with OR = 4,000 for A. The allelic frequencies for TNF-α-308 in the patients were 92.5% for G and 7.5% for A.
Conclusions: There weren’t any statistically significant associations. The allele A of the TNF-α-238 polymorphism resulted in a considerable risk factor for prostate cancer. |
| first_indexed | 2024-03-12T07:35:01Z |
| format | Article |
| id | doaj.art-4f7d2b95847342b180ae605834fa1df4 |
| institution | Directory Open Access Journal |
| issn | 0002-5151 2448-9190 |
| language | English |
| last_indexed | 2024-03-12T07:35:01Z |
| publishDate | 2019-06-01 |
| publisher | Colegio Mexicano de Inmunología Clínica y Alergia, A.C. |
| record_format | Article |
| series | Revista Alergia México |
| spelling | doaj.art-4f7d2b95847342b180ae605834fa1df42023-09-02T21:32:55ZengColegio Mexicano de Inmunología Clínica y Alergia, A.C.Revista Alergia México0002-51512448-91902019-06-0166215416210.29262/ram.v66i2.517407Study of the association between the polymorphism of the TNF-α gene and prostate cancerTatiana Pardo0Pedro Salcedo1José Miguel Quintero2Lisbeth Borjas3Mercedes Fernández-Mestre4Yanira Sánchez5Zuhey Carrillo6Sergio Rivera7Universidad del Zulia, Facultad de Medicina, Instituto de Investigaciones Genéticas, MaracaiboLaboratorio HLA Inmunología y Genética, Instituto Hematológico de Occidente, Banco de Sangre del Estado Zulia, ZuliaUniversidad del Zulia, Facultad de Medicina, Instituto de Investigaciones Genéticas, MaracaiboUniversidad del Zulia, Facultad de Medicina, Instituto de Investigaciones Genéticas, MaracaiboInstituto Venezolano de Investigaciones Científicas, Centro de Medicina Experimental, Laboratorio de Fisiopatología, MirandaUniversidad del Zulia, Facultad de Medicina, Instituto de Investigaciones Genéticas, MaracaiboLaboratorio HLA Inmunología y Genética, Instituto Hematológico de Occidente, Banco de Sangre del Estado Zulia, Maracaibo, Venezuela.Laboratorio HLA Inmunología y Genética, Instituto Hematológico de Occidente, Banco de Sangre del Estado Zulia, ZuliaBackground: Prostate cancer is the third cause of cancer death in men in the Western hemisphere and the second cause of cancer death in Zulian men from Venezuela. Objective: To determine whether polymorphisms 308 and 238 of the tumor necrosis factor alpha (TNF-α) gene are associated with prostate cancer. Methods: The DNA that was extracted from the peripheral blood of 40 patients with prostatic specific antigen and 40 controls was amplified by PCR plus digestion with enzymes NcoI and MspI. Results: In the patients, genotypes of the TNF-α-238 polymorphism were observed in 90% GG and 10% GA; in controls, in 97.5% GG and 2.5% GA, odds ratio (OR) = 4,000 for GA. In the patients, genotypes of TNF-α-308 polymorphism were identified in 85% GG and 15% GA, and in controls in 72.5% GG and 27.5% GA, OR = 0.545 for GA and 1.172 for GG. The allelic frequencies for TNF-α-238 in patients were 95% for G and 5% for A; in controls, 98.75% for G and 1.25% for A, with OR = 4,000 for A. The allelic frequencies for TNF-α-308 in the patients were 92.5% for G and 7.5% for A. Conclusions: There weren’t any statistically significant associations. The allele A of the TNF-α-238 polymorphism resulted in a considerable risk factor for prostate cancer.http://revistaalergia.mx/ojs/index.php/ram/article/view/517cáncer de próstatatnf-αpolimorfismo tnf-αpolimorfismo tnfα-308antígeno prostático específico |
| spellingShingle | Tatiana Pardo Pedro Salcedo José Miguel Quintero Lisbeth Borjas Mercedes Fernández-Mestre Yanira Sánchez Zuhey Carrillo Sergio Rivera Study of the association between the polymorphism of the TNF-α gene and prostate cancer Revista Alergia México cáncer de próstata tnf-α polimorfismo tnf-α polimorfismo tnfα-308 antígeno prostático específico |
| title | Study of the association between the polymorphism of the TNF-α gene and prostate cancer |
| title_full | Study of the association between the polymorphism of the TNF-α gene and prostate cancer |
| title_fullStr | Study of the association between the polymorphism of the TNF-α gene and prostate cancer |
| title_full_unstemmed | Study of the association between the polymorphism of the TNF-α gene and prostate cancer |
| title_short | Study of the association between the polymorphism of the TNF-α gene and prostate cancer |
| title_sort | study of the association between the polymorphism of the tnf α gene and prostate cancer |
| topic | cáncer de próstata tnf-α polimorfismo tnf-α polimorfismo tnfα-308 antígeno prostático específico |
| url | http://revistaalergia.mx/ojs/index.php/ram/article/view/517 |
| work_keys_str_mv | AT tatianapardo studyoftheassociationbetweenthepolymorphismofthetnfageneandprostatecancer AT pedrosalcedo studyoftheassociationbetweenthepolymorphismofthetnfageneandprostatecancer AT josemiguelquintero studyoftheassociationbetweenthepolymorphismofthetnfageneandprostatecancer AT lisbethborjas studyoftheassociationbetweenthepolymorphismofthetnfageneandprostatecancer AT mercedesfernandezmestre studyoftheassociationbetweenthepolymorphismofthetnfageneandprostatecancer AT yanirasanchez studyoftheassociationbetweenthepolymorphismofthetnfageneandprostatecancer AT zuheycarrillo studyoftheassociationbetweenthepolymorphismofthetnfageneandprostatecancer AT sergiorivera studyoftheassociationbetweenthepolymorphismofthetnfageneandprostatecancer |