Regulation of bone and fat balance by Fructus Ligustri Lucidi in ovariectomized mice
AbstractContext Fructus Ligustri Lucidi (FLL), a commonly used herb of traditional Chinese medicine (TCM), is the fruit of Ligustrum lucidum Ait. (Oleaceae). The ethanol extract of FLL is a potential candidate for preventing and treating postmenopausal osteoporosis (PMOP) by nourishing the liver and...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/13880209.2023.2168019 |
| _version_ | 1797234828082413568 |
|---|---|
| author | Xiaoyan Qin Qiu Wei Ran An Yun Yang Mingqi Cai Xiaoling Han Haoping Mao Xiumei Gao |
| author_facet | Xiaoyan Qin Qiu Wei Ran An Yun Yang Mingqi Cai Xiaoling Han Haoping Mao Xiumei Gao |
| author_sort | Xiaoyan Qin |
| collection | DOAJ |
| description | AbstractContext Fructus Ligustri Lucidi (FLL), a commonly used herb of traditional Chinese medicine (TCM), is the fruit of Ligustrum lucidum Ait. (Oleaceae). The ethanol extract of FLL is a potential candidate for preventing and treating postmenopausal osteoporosis (PMOP) by nourishing the liver and kidneys.Objective This study determines whether an ethanol extract of FLL has anti-osteoporotic effects in ovariectomized (OVX) mice and explores the underlying mechanism.Materials and methods The OVX model of eight-week-old C57BL/6J female mice was taken, and ovariectomy was used as PMOP. Mice were divided into five groups: sham-operated group (n = 10), OVX group (n = 10), OVX + E2 group (n = 10; 0.039 mg/kg), OVX + FLL group (n = 10; 2 g/kg) and OVX + FLL group (n = 10; 4 g/kg). Mice were treated by gavage with FLL or CMCNa once daily for 8 weeks. We harvested uteri, femur, and tibias from mice; bone mineral density (BMD) and bone microstructure were obtained by X-ray absorptiometry and micro-CT. Furthermore, the effect of FLL on the balance of osteoblast and adipocyte differentiation was investigated using bone marrow mesenchymal stem cells (BMMSCs).Results The results indicated that FLL did not affect OVX-induced estradiol reduction. Compared with OVX mice, FLL significantly increased BMD (63.54 vs. 61.96), Conn. D (86.46 vs. 57.00), and left tibial strength (13.91 vs. 11.27), decreased Tb. Sp (0.38 vs. 0.44) and body fat content (4.19% vs. 11.24%). FLL decreased osteoclast activity and enhanced RUNX2 expression; inhibited perilipin peroxisome proliferator-activated receptor gamma (PPARγ) expression and adipocyte differentiation from BMMSCs.Conclusions FLL prevented additional bone loss and improved bone microstructure in OVX mice by modulating bone and fat balance, suggesting that FLL might be a therapeutic agent for PMOP. |
| first_indexed | 2024-03-09T00:09:08Z |
| format | Article |
| id | doaj.art-4f7d641fe9714ff69aa269ad53b0cd6c |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-04-24T16:38:16Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-4f7d641fe9714ff69aa269ad53b0cd6c2024-03-29T11:10:26ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162023-12-0161139140310.1080/13880209.2023.2168019Regulation of bone and fat balance by Fructus Ligustri Lucidi in ovariectomized miceXiaoyan Qin0Qiu Wei1Ran An2Yun Yang3Mingqi Cai4Xiaoling Han5Haoping Mao6Xiumei Gao7Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaKey Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaKey Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaKey Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaKey Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaKey Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaKey Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaKey Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaAbstractContext Fructus Ligustri Lucidi (FLL), a commonly used herb of traditional Chinese medicine (TCM), is the fruit of Ligustrum lucidum Ait. (Oleaceae). The ethanol extract of FLL is a potential candidate for preventing and treating postmenopausal osteoporosis (PMOP) by nourishing the liver and kidneys.Objective This study determines whether an ethanol extract of FLL has anti-osteoporotic effects in ovariectomized (OVX) mice and explores the underlying mechanism.Materials and methods The OVX model of eight-week-old C57BL/6J female mice was taken, and ovariectomy was used as PMOP. Mice were divided into five groups: sham-operated group (n = 10), OVX group (n = 10), OVX + E2 group (n = 10; 0.039 mg/kg), OVX + FLL group (n = 10; 2 g/kg) and OVX + FLL group (n = 10; 4 g/kg). Mice were treated by gavage with FLL or CMCNa once daily for 8 weeks. We harvested uteri, femur, and tibias from mice; bone mineral density (BMD) and bone microstructure were obtained by X-ray absorptiometry and micro-CT. Furthermore, the effect of FLL on the balance of osteoblast and adipocyte differentiation was investigated using bone marrow mesenchymal stem cells (BMMSCs).Results The results indicated that FLL did not affect OVX-induced estradiol reduction. Compared with OVX mice, FLL significantly increased BMD (63.54 vs. 61.96), Conn. D (86.46 vs. 57.00), and left tibial strength (13.91 vs. 11.27), decreased Tb. Sp (0.38 vs. 0.44) and body fat content (4.19% vs. 11.24%). FLL decreased osteoclast activity and enhanced RUNX2 expression; inhibited perilipin peroxisome proliferator-activated receptor gamma (PPARγ) expression and adipocyte differentiation from BMMSCs.Conclusions FLL prevented additional bone loss and improved bone microstructure in OVX mice by modulating bone and fat balance, suggesting that FLL might be a therapeutic agent for PMOP.https://www.tandfonline.com/doi/10.1080/13880209.2023.2168019Ovariectomyosteoblastogenesisadipogenesispostmenopausal osteoporosis (PMOP)bone marrow mesenchymal stem cells (BMMSCs) |
| spellingShingle | Xiaoyan Qin Qiu Wei Ran An Yun Yang Mingqi Cai Xiaoling Han Haoping Mao Xiumei Gao Regulation of bone and fat balance by Fructus Ligustri Lucidi in ovariectomized mice Pharmaceutical Biology Ovariectomy osteoblastogenesis adipogenesis postmenopausal osteoporosis (PMOP) bone marrow mesenchymal stem cells (BMMSCs) |
| title | Regulation of bone and fat balance by Fructus Ligustri Lucidi in ovariectomized mice |
| title_full | Regulation of bone and fat balance by Fructus Ligustri Lucidi in ovariectomized mice |
| title_fullStr | Regulation of bone and fat balance by Fructus Ligustri Lucidi in ovariectomized mice |
| title_full_unstemmed | Regulation of bone and fat balance by Fructus Ligustri Lucidi in ovariectomized mice |
| title_short | Regulation of bone and fat balance by Fructus Ligustri Lucidi in ovariectomized mice |
| title_sort | regulation of bone and fat balance by fructus ligustri lucidi in ovariectomized mice |
| topic | Ovariectomy osteoblastogenesis adipogenesis postmenopausal osteoporosis (PMOP) bone marrow mesenchymal stem cells (BMMSCs) |
| url | https://www.tandfonline.com/doi/10.1080/13880209.2023.2168019 |
| work_keys_str_mv | AT xiaoyanqin regulationofboneandfatbalancebyfructusligustrilucidiinovariectomizedmice AT qiuwei regulationofboneandfatbalancebyfructusligustrilucidiinovariectomizedmice AT ranan regulationofboneandfatbalancebyfructusligustrilucidiinovariectomizedmice AT yunyang regulationofboneandfatbalancebyfructusligustrilucidiinovariectomizedmice AT mingqicai regulationofboneandfatbalancebyfructusligustrilucidiinovariectomizedmice AT xiaolinghan regulationofboneandfatbalancebyfructusligustrilucidiinovariectomizedmice AT haopingmao regulationofboneandfatbalancebyfructusligustrilucidiinovariectomizedmice AT xiumeigao regulationofboneandfatbalancebyfructusligustrilucidiinovariectomizedmice |