Combination of 16S rRNA and procalcitonin in diagnosis of neonatal clinically suspected sepsis
Objective To investigate the application of 16S rRNA in diagnosing patients with neonatal sepsis. Methods We studied 60 consecutive neonatal patients with clinically suspected sepsis and 20 non-infective cases as controls. All patients were diagnosed with sepsis by clinical and experimental criteria...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2020-10-01
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| Series: | Journal of International Medical Research |
| Online Access: | https://doi.org/10.1177/0300060519892418 |
| _version_ | 1819140989605904384 |
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| author | Renqiang Yu Qin Zhou Shanyu Jiang Yingzi Mei Min Wang |
| author_facet | Renqiang Yu Qin Zhou Shanyu Jiang Yingzi Mei Min Wang |
| author_sort | Renqiang Yu |
| collection | DOAJ |
| description | Objective To investigate the application of 16S rRNA in diagnosing patients with neonatal sepsis. Methods We studied 60 consecutive neonatal patients with clinically suspected sepsis and 20 non-infective cases as controls. All patients were diagnosed with sepsis by clinical and experimental criteria. Clinical characteristics were recorded and 16S rRNA sequencing was conducted for all patients. The sensitivity, specificity, and accuracy of the detection methods were analyzed. Results The detection limit of 16S rRNA sequencing was 1 × 10 2 CFU/mL. For suspected sepsis, the positive rate of 16S rRNA detection was 93.3%, which was similar to that of procalcitonin detection (85%), and was significantly higher than that of bacterial culture (51.7%). The specificity of procalcitonin detection (74.1%) was significantly lower than that of 16S rRNA detection (100%). Moreover, the combination of 16S rRNA and procalcitonin detection showed a sensitivity of 100%, specificity of 74.1%, and accuracy of 92.0%. For proven sepsis, the sensitivity and specificity of 16S rRNA detection were both 100.0%, and those for procalcitonin were 87.1% and 87.0%, respectively. Conclusion Detection of 16S rRNA has high sensitivity and specificity in diagnosing sepsis. The combination of 16S rRNA and procalcitonin has even better sensitivity with acceptable specificity. |
| first_indexed | 2024-12-22T11:47:19Z |
| format | Article |
| id | doaj.art-4f7e784272bb41a6b63c0edf54a01948 |
| institution | Directory Open Access Journal |
| issn | 1473-2300 |
| language | English |
| last_indexed | 2024-12-22T11:47:19Z |
| publishDate | 2020-10-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of International Medical Research |
| spelling | doaj.art-4f7e784272bb41a6b63c0edf54a019482022-12-21T18:27:07ZengSAGE PublishingJournal of International Medical Research1473-23002020-10-014810.1177/0300060519892418Combination of 16S rRNA and procalcitonin in diagnosis of neonatal clinically suspected sepsisRenqiang YuQin ZhouShanyu JiangYingzi MeiMin WangObjective To investigate the application of 16S rRNA in diagnosing patients with neonatal sepsis. Methods We studied 60 consecutive neonatal patients with clinically suspected sepsis and 20 non-infective cases as controls. All patients were diagnosed with sepsis by clinical and experimental criteria. Clinical characteristics were recorded and 16S rRNA sequencing was conducted for all patients. The sensitivity, specificity, and accuracy of the detection methods were analyzed. Results The detection limit of 16S rRNA sequencing was 1 × 10 2 CFU/mL. For suspected sepsis, the positive rate of 16S rRNA detection was 93.3%, which was similar to that of procalcitonin detection (85%), and was significantly higher than that of bacterial culture (51.7%). The specificity of procalcitonin detection (74.1%) was significantly lower than that of 16S rRNA detection (100%). Moreover, the combination of 16S rRNA and procalcitonin detection showed a sensitivity of 100%, specificity of 74.1%, and accuracy of 92.0%. For proven sepsis, the sensitivity and specificity of 16S rRNA detection were both 100.0%, and those for procalcitonin were 87.1% and 87.0%, respectively. Conclusion Detection of 16S rRNA has high sensitivity and specificity in diagnosing sepsis. The combination of 16S rRNA and procalcitonin has even better sensitivity with acceptable specificity.https://doi.org/10.1177/0300060519892418 |
| spellingShingle | Renqiang Yu Qin Zhou Shanyu Jiang Yingzi Mei Min Wang Combination of 16S rRNA and procalcitonin in diagnosis of neonatal clinically suspected sepsis Journal of International Medical Research |
| title | Combination of 16S rRNA and procalcitonin in diagnosis of neonatal clinically suspected sepsis |
| title_full | Combination of 16S rRNA and procalcitonin in diagnosis of neonatal clinically suspected sepsis |
| title_fullStr | Combination of 16S rRNA and procalcitonin in diagnosis of neonatal clinically suspected sepsis |
| title_full_unstemmed | Combination of 16S rRNA and procalcitonin in diagnosis of neonatal clinically suspected sepsis |
| title_short | Combination of 16S rRNA and procalcitonin in diagnosis of neonatal clinically suspected sepsis |
| title_sort | combination of 16s rrna and procalcitonin in diagnosis of neonatal clinically suspected sepsis |
| url | https://doi.org/10.1177/0300060519892418 |
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