Membrane lipids define small extracellular vesicle subtypes secreted by mesenchymal stromal cells
The therapeutic efficacy of mesenchymal stromal cells (MSCs), multipotent progenitor cells, is attributed to small (50–200 nm) extracellular vesicles (EVs). The presence of a lipid membrane differentiates exosomes and EVs from other macromolecules. Analysis of this lipid membrane revealed three dist...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-02-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520326432 |
| _version_ | 1818412029698899968 |
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| author | Ruenn Chai Lai Sai Kiang Lim |
| author_facet | Ruenn Chai Lai Sai Kiang Lim |
| author_sort | Ruenn Chai Lai |
| collection | DOAJ |
| description | The therapeutic efficacy of mesenchymal stromal cells (MSCs), multipotent progenitor cells, is attributed to small (50–200 nm) extracellular vesicles (EVs). The presence of a lipid membrane differentiates exosomes and EVs from other macromolecules. Analysis of this lipid membrane revealed three distinct small MSC EV subtypes, each with a differential affinity for cholera toxin B chain (CTB), annexin V (AV), and Shiga toxin B chain (ST) that bind GM1 ganglioside, phosphatidylserine, and globotriaosylceramide, respectively. Similar EV subtypes are also found in biologic fluids and are independent sources of disease biomarkers. Here, we compare and contrast these three EV subtypes. All subtypes carry β-actin, but only CTB-binding EVs (CTB-EVs) are true exosomes, enriched with exosome proteins and derived from endosomes. No unique protein has been identified yet in AV-binding EVs (AV-EVs); ST-binding EVs (ST-EVs) carry RNA and a high level of extra domain A-containing fibronectin. Based on the CTB, AV, and ST subcellular binding sites, the origins of CTB-, AV-, and ST-EV biogenesis are the plasma membrane, cytoplasm, and nucleus, respectively. The differentiation of EV subtypes through membrane lipids underlies the importance of membrane lipids in defining EVs and implies an influence on EV biology and functions. |
| first_indexed | 2024-12-14T10:40:49Z |
| format | Article |
| id | doaj.art-4f80f9bbc5e04ee5ba46fcaf695ef1e0 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-14T10:40:49Z |
| publishDate | 2019-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-4f80f9bbc5e04ee5ba46fcaf695ef1e02022-12-21T23:05:41ZengElsevierJournal of Lipid Research0022-22752019-02-01602318322Membrane lipids define small extracellular vesicle subtypes secreted by mesenchymal stromal cellsRuenn Chai Lai0Sai Kiang Lim1A*STAR Institute of Medical Biology, S138648 SingaporeTo whom correspondence should be addressed; A*STAR Institute of Medical Biology, S138648 Singapore; Department of Surgery Yong Loo Lin School of Medicine, National University of Singapore, S119074 SingaporeThe therapeutic efficacy of mesenchymal stromal cells (MSCs), multipotent progenitor cells, is attributed to small (50–200 nm) extracellular vesicles (EVs). The presence of a lipid membrane differentiates exosomes and EVs from other macromolecules. Analysis of this lipid membrane revealed three distinct small MSC EV subtypes, each with a differential affinity for cholera toxin B chain (CTB), annexin V (AV), and Shiga toxin B chain (ST) that bind GM1 ganglioside, phosphatidylserine, and globotriaosylceramide, respectively. Similar EV subtypes are also found in biologic fluids and are independent sources of disease biomarkers. Here, we compare and contrast these three EV subtypes. All subtypes carry β-actin, but only CTB-binding EVs (CTB-EVs) are true exosomes, enriched with exosome proteins and derived from endosomes. No unique protein has been identified yet in AV-binding EVs (AV-EVs); ST-binding EVs (ST-EVs) carry RNA and a high level of extra domain A-containing fibronectin. Based on the CTB, AV, and ST subcellular binding sites, the origins of CTB-, AV-, and ST-EV biogenesis are the plasma membrane, cytoplasm, and nucleus, respectively. The differentiation of EV subtypes through membrane lipids underlies the importance of membrane lipids in defining EVs and implies an influence on EV biology and functions.http://www.sciencedirect.com/science/article/pii/S0022227520326432cholesterol/traffickingendocytosissecretionexosomecholera toxin B chainannexin V |
| spellingShingle | Ruenn Chai Lai Sai Kiang Lim Membrane lipids define small extracellular vesicle subtypes secreted by mesenchymal stromal cells Journal of Lipid Research cholesterol/trafficking endocytosis secretion exosome cholera toxin B chain annexin V |
| title | Membrane lipids define small extracellular vesicle subtypes secreted by mesenchymal stromal cells |
| title_full | Membrane lipids define small extracellular vesicle subtypes secreted by mesenchymal stromal cells |
| title_fullStr | Membrane lipids define small extracellular vesicle subtypes secreted by mesenchymal stromal cells |
| title_full_unstemmed | Membrane lipids define small extracellular vesicle subtypes secreted by mesenchymal stromal cells |
| title_short | Membrane lipids define small extracellular vesicle subtypes secreted by mesenchymal stromal cells |
| title_sort | membrane lipids define small extracellular vesicle subtypes secreted by mesenchymal stromal cells |
| topic | cholesterol/trafficking endocytosis secretion exosome cholera toxin B chain annexin V |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520326432 |
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