DETECTION OF PIG MEAT, LIVER AND LARD IN BEEF BY CE-SSCP
Identification of animal species from foodstuffs is important in order to identify frauds to prevent substitutions and admixtures in animal products. In this paper we demonstrate the identification of cattle and pig species by polymerase chain reaction (PCR) capillary electrophoresis - single strand...
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| Format: | Article |
| Language: | English |
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Faculty of Agrobitechnical Sciences Osijek
2015-09-01
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| Series: | Poljoprivreda |
| Subjects: | |
| Online Access: | http://poljoprivreda.pfos.hr/upload/publications/poljoprivreda-21-1-sup50.pdf |
| _version_ | 1818309880780423168 |
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| author | Ádám Simon Ákos Tisza Ádám Csikós András Jávor Levente Czeglédi |
| author_facet | Ádám Simon Ákos Tisza Ádám Csikós András Jávor Levente Czeglédi |
| author_sort | Ádám Simon |
| collection | DOAJ |
| description | Identification of animal species from foodstuffs is important in order to identify frauds to prevent substitutions and admixtures in animal products. In this paper we demonstrate the identification of cattle and pig species by polymerase chain reaction (PCR) capillary electrophoresis - single stranded conformation polymorphism (CE-SSCP) method. The procedure is based on the amplification of the 12S rRNA gene encoded in the mitochondrial DNA (mtDNA). Since mtDNA copy number is highly tissue dependent mixtures of different pig tissues in cattle meat were prepared, at a concentration of 1, 5, 10, 20 w/w% of pig lard, liver and loin. It was determined that regardless the tissue type pig DNA can be detected by CE-SSCP at each contamination level. |
| first_indexed | 2024-12-13T07:37:12Z |
| format | Article |
| id | doaj.art-4f83643b9ceb402b80a5d0e4b22834ae |
| institution | Directory Open Access Journal |
| issn | 1330-7142 1848-8080 |
| language | English |
| last_indexed | 2024-12-13T07:37:12Z |
| publishDate | 2015-09-01 |
| publisher | Faculty of Agrobitechnical Sciences Osijek |
| record_format | Article |
| series | Poljoprivreda |
| spelling | doaj.art-4f83643b9ceb402b80a5d0e4b22834ae2022-12-21T23:55:03ZengFaculty of Agrobitechnical Sciences OsijekPoljoprivreda1330-71421848-80802015-09-01211 sup21221510.18047/poljo.21.1.sup.50DETECTION OF PIG MEAT, LIVER AND LARD IN BEEF BY CE-SSCPÁdám Simon0Ákos Tisza1Ádám Csikós2András Jávor3Levente Czeglédi4University of Debrecen, Faculty of Agriculture, Food Science and Environmental Management, Department of Animal Science, 138. Boszormenyi Street, H4032 Debrecen, HungaryUniversity of Debrecen, Faculty of Agriculture, Food Science and Environmental Management, Department of Animal Science, 138. Boszormenyi Street, H4032 Debrecen, Hungary University of Debrecen, Faculty of Agriculture, Food Science and Environmental Management, Department of Animal Science, 138. Boszormenyi Street, H4032 Debrecen, Hungary University of Debrecen, Faculty of Agriculture, Food Science and Environmental Management, Department of Animal Science, 138. Boszormenyi Street, H4032 Debrecen, Hungary University of Debrecen, Faculty of Agriculture, Food Science and Environmental Management, Department of Animal Science, 138. Boszormenyi Street, H4032 Debrecen, Hungary Identification of animal species from foodstuffs is important in order to identify frauds to prevent substitutions and admixtures in animal products. In this paper we demonstrate the identification of cattle and pig species by polymerase chain reaction (PCR) capillary electrophoresis - single stranded conformation polymorphism (CE-SSCP) method. The procedure is based on the amplification of the 12S rRNA gene encoded in the mitochondrial DNA (mtDNA). Since mtDNA copy number is highly tissue dependent mixtures of different pig tissues in cattle meat were prepared, at a concentration of 1, 5, 10, 20 w/w% of pig lard, liver and loin. It was determined that regardless the tissue type pig DNA can be detected by CE-SSCP at each contamination level.http://poljoprivreda.pfos.hr/upload/publications/poljoprivreda-21-1-sup50.pdfCE-SSCPmtDNAspecies identification12S rRNA |
| spellingShingle | Ádám Simon Ákos Tisza Ádám Csikós András Jávor Levente Czeglédi DETECTION OF PIG MEAT, LIVER AND LARD IN BEEF BY CE-SSCP Poljoprivreda CE-SSCP mtDNA species identification 12S rRNA |
| title | DETECTION OF PIG MEAT, LIVER AND LARD IN BEEF BY CE-SSCP |
| title_full | DETECTION OF PIG MEAT, LIVER AND LARD IN BEEF BY CE-SSCP |
| title_fullStr | DETECTION OF PIG MEAT, LIVER AND LARD IN BEEF BY CE-SSCP |
| title_full_unstemmed | DETECTION OF PIG MEAT, LIVER AND LARD IN BEEF BY CE-SSCP |
| title_short | DETECTION OF PIG MEAT, LIVER AND LARD IN BEEF BY CE-SSCP |
| title_sort | detection of pig meat liver and lard in beef by ce sscp |
| topic | CE-SSCP mtDNA species identification 12S rRNA |
| url | http://poljoprivreda.pfos.hr/upload/publications/poljoprivreda-21-1-sup50.pdf |
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