Reproduction in porcine circovirus type 2 (PCV2) seropositive gilts inseminated with PCV2b spiked semen
<p>Abstract</p> <p>Background</p> <p>Since 1999, field evidence of transplacental infection by porcine circovirus type 2 (PCV2) and reproductive failure has been reported in pigs. The objective of this study was to evaluate the clinical and pathological consequences of...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-08-01
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| Series: | Acta Veterinaria Scandinavica |
| Subjects: | |
| Online Access: | http://www.actavetscand.com/content/54/1/51 |
| _version_ | 1818145842923569152 |
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| author | Sarli Giuseppe Morandi Federico Panarese Serena Bacci Barbara Ferrara Domenico Bianco Carlo Fusaro Laura Bacci Maria Galeati Giovanna Dottori Michele Bonilauri Paolo Lelli Davide Leotti Giorgio Vila Thais Joisel Francois Allan Gordon Benazzi Cinzia Ostanello Fabio |
| author_facet | Sarli Giuseppe Morandi Federico Panarese Serena Bacci Barbara Ferrara Domenico Bianco Carlo Fusaro Laura Bacci Maria Galeati Giovanna Dottori Michele Bonilauri Paolo Lelli Davide Leotti Giorgio Vila Thais Joisel Francois Allan Gordon Benazzi Cinzia Ostanello Fabio |
| author_sort | Sarli Giuseppe |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Since 1999, field evidence of transplacental infection by porcine circovirus type 2 (PCV2) and reproductive failure has been reported in pigs. The objective of this study was to evaluate the clinical and pathological consequences of PCV2 infection in conventional PCV2-seropositive gilts by insemination with PCV2b-spiked semen.</p> <p>Results</p> <p>Six PCV2 seropositive gilts were inseminated with PCV2b-supplemented semen (infected) and three animals with semen and cell culture medium (controls). Only three out of the six infected animals were pregnant by ultrasonography on day 29 after insemination, while two out of the three controls were pregnant. One control gilt aborted on day 23 after insemination but not due to PVC2. Viraemia was demonstrated in four out of six infected and in one control gilt that became infected with PCV2a. Anti-PCV2 antibody titres showed dynamic variations in the infected group throughout the study. Among infected gilts, the animal with the lowest anti-PCV2 titre (1/100) at the beginning of the experiment and another that reached a similar low value during the experiment showed evident seroconversion over time and had also PCV2 positive foetuses. One placenta displayed mild focal necrosis of the chorionic epithelium positively stained by immunohistochemistry for PCV2 antigen.</p> <p>Conclusions</p> <p>PCV2-seropositive gilts can be infected with PCV2 after intrauterine exposure and low maternal antibody titre may increase the probability of a foetal infection.</p> |
| first_indexed | 2024-12-11T12:09:53Z |
| format | Article |
| id | doaj.art-4f8693fd9b0c451db21e5e88a6e17ec8 |
| institution | Directory Open Access Journal |
| issn | 1751-0147 |
| language | English |
| last_indexed | 2024-12-11T12:09:53Z |
| publishDate | 2012-08-01 |
| publisher | BMC |
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| series | Acta Veterinaria Scandinavica |
| spelling | doaj.art-4f8693fd9b0c451db21e5e88a6e17ec82022-12-22T01:07:50ZengBMCActa Veterinaria Scandinavica1751-01472012-08-015415110.1186/1751-0147-54-51Reproduction in porcine circovirus type 2 (PCV2) seropositive gilts inseminated with PCV2b spiked semenSarli GiuseppeMorandi FedericoPanarese SerenaBacci BarbaraFerrara DomenicoBianco CarloFusaro LauraBacci MariaGaleati GiovannaDottori MicheleBonilauri PaoloLelli DavideLeotti GiorgioVila ThaisJoisel FrancoisAllan GordonBenazzi CinziaOstanello Fabio<p>Abstract</p> <p>Background</p> <p>Since 1999, field evidence of transplacental infection by porcine circovirus type 2 (PCV2) and reproductive failure has been reported in pigs. The objective of this study was to evaluate the clinical and pathological consequences of PCV2 infection in conventional PCV2-seropositive gilts by insemination with PCV2b-spiked semen.</p> <p>Results</p> <p>Six PCV2 seropositive gilts were inseminated with PCV2b-supplemented semen (infected) and three animals with semen and cell culture medium (controls). Only three out of the six infected animals were pregnant by ultrasonography on day 29 after insemination, while two out of the three controls were pregnant. One control gilt aborted on day 23 after insemination but not due to PVC2. Viraemia was demonstrated in four out of six infected and in one control gilt that became infected with PCV2a. Anti-PCV2 antibody titres showed dynamic variations in the infected group throughout the study. Among infected gilts, the animal with the lowest anti-PCV2 titre (1/100) at the beginning of the experiment and another that reached a similar low value during the experiment showed evident seroconversion over time and had also PCV2 positive foetuses. One placenta displayed mild focal necrosis of the chorionic epithelium positively stained by immunohistochemistry for PCV2 antigen.</p> <p>Conclusions</p> <p>PCV2-seropositive gilts can be infected with PCV2 after intrauterine exposure and low maternal antibody titre may increase the probability of a foetal infection.</p>http://www.actavetscand.com/content/54/1/51SwinePorcine circovirus type 2SemenInfection |
| spellingShingle | Sarli Giuseppe Morandi Federico Panarese Serena Bacci Barbara Ferrara Domenico Bianco Carlo Fusaro Laura Bacci Maria Galeati Giovanna Dottori Michele Bonilauri Paolo Lelli Davide Leotti Giorgio Vila Thais Joisel Francois Allan Gordon Benazzi Cinzia Ostanello Fabio Reproduction in porcine circovirus type 2 (PCV2) seropositive gilts inseminated with PCV2b spiked semen Acta Veterinaria Scandinavica Swine Porcine circovirus type 2 Semen Infection |
| title | Reproduction in porcine circovirus type 2 (PCV2) seropositive gilts inseminated with PCV2b spiked semen |
| title_full | Reproduction in porcine circovirus type 2 (PCV2) seropositive gilts inseminated with PCV2b spiked semen |
| title_fullStr | Reproduction in porcine circovirus type 2 (PCV2) seropositive gilts inseminated with PCV2b spiked semen |
| title_full_unstemmed | Reproduction in porcine circovirus type 2 (PCV2) seropositive gilts inseminated with PCV2b spiked semen |
| title_short | Reproduction in porcine circovirus type 2 (PCV2) seropositive gilts inseminated with PCV2b spiked semen |
| title_sort | reproduction in porcine circovirus type 2 pcv2 seropositive gilts inseminated with pcv2b spiked semen |
| topic | Swine Porcine circovirus type 2 Semen Infection |
| url | http://www.actavetscand.com/content/54/1/51 |
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