Antimicrobial Potential of Endophytic Fungi From Artemisia argyi and Bioactive Metabolites From Diaporthe sp. AC1
Endophytic fungi of medicinal plants are important sources of active natural products. In this study, 26 fungi were isolated from Artemisia argyi, which were belonging to eight genera, namely, Alternaria, Fusarium, Chaetomium, Phoma, Diaporthe, Trichoderma, Gibberella, and Colletotrichum. The antimi...
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| Format: | Article |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.908836/full |
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| author | Haiping Gu Shikai Zhang Lin Liu Zhengyou Yang Fengchun Zhao Yuan Tian |
| author_facet | Haiping Gu Shikai Zhang Lin Liu Zhengyou Yang Fengchun Zhao Yuan Tian |
| author_sort | Haiping Gu |
| collection | DOAJ |
| description | Endophytic fungi of medicinal plants are important sources of active natural products. In this study, 26 fungi were isolated from Artemisia argyi, which were belonging to eight genera, namely, Alternaria, Fusarium, Chaetomium, Phoma, Diaporthe, Trichoderma, Gibberella, and Colletotrichum. The antimicrobial activities of all fungal extracts were tested by using the cup-plate method against Staphylococcus aureus, Salmonella enteritidis, and Fusarium graminearum. The results demonstrated that 25 extracts (96%) exhibited inhibitory activity against at least one of the tested pathogenic microorganisms. The strain Diaporthe sp. AC1, which showed good antimicrobial activity and high yield of crude extract from fermentation, was selected for the study of secondary metabolites. The crude extract of strain AC1 was purified by silica gel column chromatography, Sephadex LH-20 gel column chromatography, and HPLC, and finally, a new compound phomopsolide G (1), together with three known phomopsolides (2–4) and four other known compounds (5–8), was obtained. The structures of the compounds were elucidated by NMR and/or HR-MS spectroscopy. Microdilution method and MTT colorimetry were used to determine the bioactivity of the compounds. The study demonstrated that the new compound 1 had moderate antifungal activity against F. graminearum, Fusarium moniliforme, and Botrytis cinerea and weak antibacterial activity against Staphylococcus aureus. Compound 1 also showed weak cytotoxicity against HepG2, A549, and MDA-MB-231, with IC50 values of 89.91, 107.65, and 53.97 μM. Additionally, other compounds also exhibited antimicrobial and/or cytotoxic activities. The findings provided the basis for searching drug and agricultural lead compounds from A. argyi-associated fungi resources. |
| first_indexed | 2024-12-12T17:23:19Z |
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| language | English |
| last_indexed | 2024-12-12T17:23:19Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-4f876687c03147b2b7851fe0cfbe10d22022-12-22T00:17:35ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-06-011310.3389/fmicb.2022.908836908836Antimicrobial Potential of Endophytic Fungi From Artemisia argyi and Bioactive Metabolites From Diaporthe sp. AC1Haiping Gu0Shikai Zhang1Lin Liu2Zhengyou Yang3Fengchun Zhao4Yuan Tian5Key Laboratory for Agriculture Microbiology, Department of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian, ChinaKey Laboratory for Agriculture Microbiology, Department of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian, ChinaKey Laboratory for Agriculture Microbiology, Department of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian, ChinaKey Laboratory for Agriculture Microbiology, Department of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian, ChinaKey Laboratory for Agriculture Microbiology, Department of Microbiology, College of Life Sciences, Shandong Agricultural University, Taian, ChinaCollege of Life Sciences, Shandong First Medical University and Shandong Academy of Medical Sciences, Taian, ChinaEndophytic fungi of medicinal plants are important sources of active natural products. In this study, 26 fungi were isolated from Artemisia argyi, which were belonging to eight genera, namely, Alternaria, Fusarium, Chaetomium, Phoma, Diaporthe, Trichoderma, Gibberella, and Colletotrichum. The antimicrobial activities of all fungal extracts were tested by using the cup-plate method against Staphylococcus aureus, Salmonella enteritidis, and Fusarium graminearum. The results demonstrated that 25 extracts (96%) exhibited inhibitory activity against at least one of the tested pathogenic microorganisms. The strain Diaporthe sp. AC1, which showed good antimicrobial activity and high yield of crude extract from fermentation, was selected for the study of secondary metabolites. The crude extract of strain AC1 was purified by silica gel column chromatography, Sephadex LH-20 gel column chromatography, and HPLC, and finally, a new compound phomopsolide G (1), together with three known phomopsolides (2–4) and four other known compounds (5–8), was obtained. The structures of the compounds were elucidated by NMR and/or HR-MS spectroscopy. Microdilution method and MTT colorimetry were used to determine the bioactivity of the compounds. The study demonstrated that the new compound 1 had moderate antifungal activity against F. graminearum, Fusarium moniliforme, and Botrytis cinerea and weak antibacterial activity against Staphylococcus aureus. Compound 1 also showed weak cytotoxicity against HepG2, A549, and MDA-MB-231, with IC50 values of 89.91, 107.65, and 53.97 μM. Additionally, other compounds also exhibited antimicrobial and/or cytotoxic activities. The findings provided the basis for searching drug and agricultural lead compounds from A. argyi-associated fungi resources.https://www.frontiersin.org/articles/10.3389/fmicb.2022.908836/fullArtemisia argyiendophytic fungiantimicrobial activitycytotoxic activitysecondary metabolites |
| spellingShingle | Haiping Gu Shikai Zhang Lin Liu Zhengyou Yang Fengchun Zhao Yuan Tian Antimicrobial Potential of Endophytic Fungi From Artemisia argyi and Bioactive Metabolites From Diaporthe sp. AC1 Frontiers in Microbiology Artemisia argyi endophytic fungi antimicrobial activity cytotoxic activity secondary metabolites |
| title | Antimicrobial Potential of Endophytic Fungi From Artemisia argyi and Bioactive Metabolites From Diaporthe sp. AC1 |
| title_full | Antimicrobial Potential of Endophytic Fungi From Artemisia argyi and Bioactive Metabolites From Diaporthe sp. AC1 |
| title_fullStr | Antimicrobial Potential of Endophytic Fungi From Artemisia argyi and Bioactive Metabolites From Diaporthe sp. AC1 |
| title_full_unstemmed | Antimicrobial Potential of Endophytic Fungi From Artemisia argyi and Bioactive Metabolites From Diaporthe sp. AC1 |
| title_short | Antimicrobial Potential of Endophytic Fungi From Artemisia argyi and Bioactive Metabolites From Diaporthe sp. AC1 |
| title_sort | antimicrobial potential of endophytic fungi from artemisia argyi and bioactive metabolites from diaporthe sp ac1 |
| topic | Artemisia argyi endophytic fungi antimicrobial activity cytotoxic activity secondary metabolites |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.908836/full |
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