Study of fortilin interacting proteins in a human skeletal muscle using the yeast two-hybrid system
Fortilin is implicated in development, cellular processes and malignant transformation. However, a unifying picture ofproteins in key processes pertaining to fortilin function is not yet emerging. To investigate the potential interactions, humanfortilin was expressed in the yeast cells, and used to...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2010-05-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://www.rdoapp.psu.ac.th/html/sjst/journal/32-2/0125-3395-32-2-137-143.pdf |
| _version_ | 1818366387465224192 |
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| author | Juntipa Purintrapiban Potchanapond Graidist |
| author_facet | Juntipa Purintrapiban Potchanapond Graidist |
| author_sort | Juntipa Purintrapiban |
| collection | DOAJ |
| description | Fortilin is implicated in development, cellular processes and malignant transformation. However, a unifying picture ofproteins in key processes pertaining to fortilin function is not yet emerging. To investigate the potential interactions, humanfortilin was expressed in the yeast cells, and used to screen for fortilin binding proteins in a human skeletal muscle. Yeastcells were transformed in the sequential procedure using yeast two-hybrid expression vector (pAS2-1) containing full-lengthhuman fortilin as ‘bait’ for the first transformation, and pGAD10 vector containing a muscle cDNA library for the secondtransformation. In addition, a direct interaction of fortilin with known proteins, Ca2+-ATPase, creatine kinase, glycogenphosphorylase, and troponin C was evaluated. -Galactosidase activity was assayed as an index of interaction betweenfortilin and the potential target proteins whereas yeast mating strategy was used to eliminate false positive and to reconfirmthe actual binding. From this analysis, eukaryotic translation elongation factor-1 delta (guanine nucleotide exchange protein)was identified as a putative fortilin interacting protein. |
| first_indexed | 2024-12-13T22:35:21Z |
| format | Article |
| id | doaj.art-4f87b85507214582938c075d8baec9e9 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-13T22:35:21Z |
| publishDate | 2010-05-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-4f87b85507214582938c075d8baec9e92022-12-21T23:28:59ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952010-05-01322137143Study of fortilin interacting proteins in a human skeletal muscle using the yeast two-hybrid systemJuntipa PurintrapibanPotchanapond GraidistFortilin is implicated in development, cellular processes and malignant transformation. However, a unifying picture ofproteins in key processes pertaining to fortilin function is not yet emerging. To investigate the potential interactions, humanfortilin was expressed in the yeast cells, and used to screen for fortilin binding proteins in a human skeletal muscle. Yeastcells were transformed in the sequential procedure using yeast two-hybrid expression vector (pAS2-1) containing full-lengthhuman fortilin as ‘bait’ for the first transformation, and pGAD10 vector containing a muscle cDNA library for the secondtransformation. In addition, a direct interaction of fortilin with known proteins, Ca2+-ATPase, creatine kinase, glycogenphosphorylase, and troponin C was evaluated. -Galactosidase activity was assayed as an index of interaction betweenfortilin and the potential target proteins whereas yeast mating strategy was used to eliminate false positive and to reconfirmthe actual binding. From this analysis, eukaryotic translation elongation factor-1 delta (guanine nucleotide exchange protein)was identified as a putative fortilin interacting protein.http://www.rdoapp.psu.ac.th/html/sjst/journal/32-2/0125-3395-32-2-137-143.pdffortilinyeast two-hybrid systemskeletal muscle cDNA libraryeukaryotic translation elongation factor-1 delta |
| spellingShingle | Juntipa Purintrapiban Potchanapond Graidist Study of fortilin interacting proteins in a human skeletal muscle using the yeast two-hybrid system Songklanakarin Journal of Science and Technology (SJST) fortilin yeast two-hybrid system skeletal muscle cDNA library eukaryotic translation elongation factor-1 delta |
| title | Study of fortilin interacting proteins in a human skeletal muscle using the yeast two-hybrid system |
| title_full | Study of fortilin interacting proteins in a human skeletal muscle using the yeast two-hybrid system |
| title_fullStr | Study of fortilin interacting proteins in a human skeletal muscle using the yeast two-hybrid system |
| title_full_unstemmed | Study of fortilin interacting proteins in a human skeletal muscle using the yeast two-hybrid system |
| title_short | Study of fortilin interacting proteins in a human skeletal muscle using the yeast two-hybrid system |
| title_sort | study of fortilin interacting proteins in a human skeletal muscle using the yeast two hybrid system |
| topic | fortilin yeast two-hybrid system skeletal muscle cDNA library eukaryotic translation elongation factor-1 delta |
| url | http://www.rdoapp.psu.ac.th/html/sjst/journal/32-2/0125-3395-32-2-137-143.pdf |
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