Design and Fabrication of a Microfluidic Chip for Particle Size-Exclusion and Enrichment
Based on the size of particles, a microfluidic chip integrating micro particles capture, controlled release and counting analysis was designed and fabricated in this paper. The chip is composed of a polydimethylsiloxane (PDMS) cover sheet and a PDMS substrate. The PDMS substrate is made of a sample...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/12/10/1218 |
| _version_ | 1797513816124162048 |
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| author | Luxia Yang Tian Ye Xiufeng Zhao Taotao Hu Yanlong Wei |
| author_facet | Luxia Yang Tian Ye Xiufeng Zhao Taotao Hu Yanlong Wei |
| author_sort | Luxia Yang |
| collection | DOAJ |
| description | Based on the size of particles, a microfluidic chip integrating micro particles capture, controlled release and counting analysis was designed and fabricated in this paper. The chip is composed of a polydimethylsiloxane (PDMS) cover sheet and a PDMS substrate. The PDMS substrate is made of a sample inlet, microfluidic channels, a micropillar array, a three-dimensional (3D) focusing channel, and a sample outlet. The chip was fabricated by the multistep SU-8 lithography and PDMS molding method in this study. The micropillar array and channels in the chip can be molded in one step and can be replicated multiple times, which reduces the production cost and increases the practicability of the chip. Using a homemade electromagnetic drive device, the detection function of the chip was tested using a deionized water solution containing 22 μm polyethylene particles. The results showed that under the action of electromagnetic force, the chip enriched polyethylene particles; when the electromagnetic force disappeared, the enriched polyethylene particles were released by injecting buffer solution, and it was looked at as new sample solution. The flow rate of the sample solution and the sheath flow solution (deionized water) was injected into the three-dimensional focusing channel at a flow rate ratio of 1:4, and the polyethylene particles sample solution was focused, which could be used for the counting and analysis of polyethylene particles. The work of this paper can provide a reference for the subsequent detection of circulating tumor cells (CTCs). |
| first_indexed | 2024-03-10T06:22:54Z |
| format | Article |
| id | doaj.art-4f88973ce8da46e4b80057ad31b204e0 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T06:22:54Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-4f88973ce8da46e4b80057ad31b204e02023-11-22T19:11:42ZengMDPI AGMicromachines2072-666X2021-10-011210121810.3390/mi12101218Design and Fabrication of a Microfluidic Chip for Particle Size-Exclusion and EnrichmentLuxia Yang0Tian Ye1Xiufeng Zhao2Taotao Hu3Yanlong Wei4Department of Computer, Taiyuan Normal University, Taiyuan 030619, ChinaDepartment of Computer, Taiyuan Normal University, Taiyuan 030619, ChinaDepartment of Computer, Taiyuan Normal University, Taiyuan 030619, ChinaDepartment of Computer, Taiyuan Normal University, Taiyuan 030619, ChinaDepartment of Computer, Taiyuan Normal University, Taiyuan 030619, ChinaBased on the size of particles, a microfluidic chip integrating micro particles capture, controlled release and counting analysis was designed and fabricated in this paper. The chip is composed of a polydimethylsiloxane (PDMS) cover sheet and a PDMS substrate. The PDMS substrate is made of a sample inlet, microfluidic channels, a micropillar array, a three-dimensional (3D) focusing channel, and a sample outlet. The chip was fabricated by the multistep SU-8 lithography and PDMS molding method in this study. The micropillar array and channels in the chip can be molded in one step and can be replicated multiple times, which reduces the production cost and increases the practicability of the chip. Using a homemade electromagnetic drive device, the detection function of the chip was tested using a deionized water solution containing 22 μm polyethylene particles. The results showed that under the action of electromagnetic force, the chip enriched polyethylene particles; when the electromagnetic force disappeared, the enriched polyethylene particles were released by injecting buffer solution, and it was looked at as new sample solution. The flow rate of the sample solution and the sheath flow solution (deionized water) was injected into the three-dimensional focusing channel at a flow rate ratio of 1:4, and the polyethylene particles sample solution was focused, which could be used for the counting and analysis of polyethylene particles. The work of this paper can provide a reference for the subsequent detection of circulating tumor cells (CTCs).https://www.mdpi.com/2072-666X/12/10/1218microfluidic chip3D focusing channelthe micropillar arrayelectromagnetic drivemicro particles |
| spellingShingle | Luxia Yang Tian Ye Xiufeng Zhao Taotao Hu Yanlong Wei Design and Fabrication of a Microfluidic Chip for Particle Size-Exclusion and Enrichment Micromachines microfluidic chip 3D focusing channel the micropillar array electromagnetic drive micro particles |
| title | Design and Fabrication of a Microfluidic Chip for Particle Size-Exclusion and Enrichment |
| title_full | Design and Fabrication of a Microfluidic Chip for Particle Size-Exclusion and Enrichment |
| title_fullStr | Design and Fabrication of a Microfluidic Chip for Particle Size-Exclusion and Enrichment |
| title_full_unstemmed | Design and Fabrication of a Microfluidic Chip for Particle Size-Exclusion and Enrichment |
| title_short | Design and Fabrication of a Microfluidic Chip for Particle Size-Exclusion and Enrichment |
| title_sort | design and fabrication of a microfluidic chip for particle size exclusion and enrichment |
| topic | microfluidic chip 3D focusing channel the micropillar array electromagnetic drive micro particles |
| url | https://www.mdpi.com/2072-666X/12/10/1218 |
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