Paraburkholderia phytofirmans PsJN delays Botrytis cinerea development on grapevine inflorescences
Grapevine flowering is an important stage in the epidemiology of Botrytis cinerea, the causal agent of gray mold disease. To prevent infection and to minimize postharvest losses, the control of this necrotrophic fungus is mainly based on chemical fungicides application. However, there is a growing i...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1030982/full |
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| author | Lidiane Carla Miotto Vilanova Marine Rondeau Mathilde Robineau Jean François Guise Céline Lavire Ludovic Vial Florence Fontaine Christophe Clément Cédric Jacquard Qassim Esmaeel Essaïd Aït Barka Lisa Sanchez |
| author_facet | Lidiane Carla Miotto Vilanova Marine Rondeau Mathilde Robineau Jean François Guise Céline Lavire Ludovic Vial Florence Fontaine Christophe Clément Cédric Jacquard Qassim Esmaeel Essaïd Aït Barka Lisa Sanchez |
| author_sort | Lidiane Carla Miotto Vilanova |
| collection | DOAJ |
| description | Grapevine flowering is an important stage in the epidemiology of Botrytis cinerea, the causal agent of gray mold disease. To prevent infection and to minimize postharvest losses, the control of this necrotrophic fungus is mainly based on chemical fungicides application. However, there is a growing interest in other control alternatives. Among them, the use of beneficial microorganisms appears as an eco-friendly strategy. This study aims to investigate the effect of Paraburkholderia phytofirmans PsJN, root-inoculated or directly sprayed on fruiting cuttings inflorescences to control B. cinerea growth. For this purpose, quantification by real time PCR of Botrytis development, direct effect of PsJN on fungal spore germination and chemotaxis were assayed. Our results showed a significant protective effect of PsJN only by direct spraying on inflorescences. Moreover, we demonstrated an inhibition exerted by PsJN on Botrytis spore germination, effective when there was a direct contact between the two microorganisms. This study showed that PsJN is positively attracted by the pathogenic fungus B. cinerea and forms a biofilm around the fungal hyphae in liquid co-culture. Finally, microscopic observations on fruit cuttings revealed a co-localization of both beneficial and pathogenic microorganisms on grapevine receptacle and stigma that might be correlated with the protective effect induced by PsJN against B. cinerea via a direct antimicrobial effect. Taking together, our findings allowed us to propose PsJN as a biofungicide to control grapevine gray mold disease. |
| first_indexed | 2024-04-12T16:29:13Z |
| format | Article |
| id | doaj.art-4f892f44179d4cfd96edae3b45455b97 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T16:29:13Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-4f892f44179d4cfd96edae3b45455b972022-12-22T03:25:14ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.10309821030982Paraburkholderia phytofirmans PsJN delays Botrytis cinerea development on grapevine inflorescencesLidiane Carla Miotto Vilanova0Marine Rondeau1Mathilde Robineau2Jean François Guise3Céline Lavire4Ludovic Vial5Florence Fontaine6Christophe Clément7Cédric Jacquard8Qassim Esmaeel9Essaïd Aït Barka10Lisa Sanchez11Université de Reims Champagne-Ardenne, RIBP USC INRAE 1488, Reims, FranceUniversité de Reims Champagne-Ardenne, RIBP USC INRAE 1488, Reims, FranceUniversité de Reims Champagne-Ardenne, RIBP USC INRAE 1488, Reims, FranceUniversité de Reims Champagne-Ardenne, RIBP USC INRAE 1488, Reims, FranceUniversité Claude Bernard Lyon 1, CNRS, INRAE, VetAgro Sup, UMR Ecologie Microbienne, Villeurbanne, FranceUniversité Claude Bernard Lyon 1, CNRS, INRAE, VetAgro Sup, UMR Ecologie Microbienne, Villeurbanne, FranceUniversité de Reims Champagne-Ardenne, RIBP USC INRAE 1488, Reims, FranceUniversité de Reims Champagne-Ardenne, RIBP USC INRAE 1488, Reims, FranceUniversité de Reims Champagne-Ardenne, RIBP USC INRAE 1488, Reims, FranceUniversité de Reims Champagne-Ardenne, RIBP USC INRAE 1488, Reims, FranceUniversité de Reims Champagne-Ardenne, RIBP USC INRAE 1488, Reims, FranceUniversité de Reims Champagne-Ardenne, RIBP USC INRAE 1488, Reims, FranceGrapevine flowering is an important stage in the epidemiology of Botrytis cinerea, the causal agent of gray mold disease. To prevent infection and to minimize postharvest losses, the control of this necrotrophic fungus is mainly based on chemical fungicides application. However, there is a growing interest in other control alternatives. Among them, the use of beneficial microorganisms appears as an eco-friendly strategy. This study aims to investigate the effect of Paraburkholderia phytofirmans PsJN, root-inoculated or directly sprayed on fruiting cuttings inflorescences to control B. cinerea growth. For this purpose, quantification by real time PCR of Botrytis development, direct effect of PsJN on fungal spore germination and chemotaxis were assayed. Our results showed a significant protective effect of PsJN only by direct spraying on inflorescences. Moreover, we demonstrated an inhibition exerted by PsJN on Botrytis spore germination, effective when there was a direct contact between the two microorganisms. This study showed that PsJN is positively attracted by the pathogenic fungus B. cinerea and forms a biofilm around the fungal hyphae in liquid co-culture. Finally, microscopic observations on fruit cuttings revealed a co-localization of both beneficial and pathogenic microorganisms on grapevine receptacle and stigma that might be correlated with the protective effect induced by PsJN against B. cinerea via a direct antimicrobial effect. Taking together, our findings allowed us to propose PsJN as a biofungicide to control grapevine gray mold disease.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1030982/fullBotrytis cinereaprotectiongrapevine inflorescencesantigerminative effectParaburkholderia phytofirmans PsJN |
| spellingShingle | Lidiane Carla Miotto Vilanova Marine Rondeau Mathilde Robineau Jean François Guise Céline Lavire Ludovic Vial Florence Fontaine Christophe Clément Cédric Jacquard Qassim Esmaeel Essaïd Aït Barka Lisa Sanchez Paraburkholderia phytofirmans PsJN delays Botrytis cinerea development on grapevine inflorescences Frontiers in Microbiology Botrytis cinerea protection grapevine inflorescences antigerminative effect Paraburkholderia phytofirmans PsJN |
| title | Paraburkholderia phytofirmans PsJN delays Botrytis cinerea development on grapevine inflorescences |
| title_full | Paraburkholderia phytofirmans PsJN delays Botrytis cinerea development on grapevine inflorescences |
| title_fullStr | Paraburkholderia phytofirmans PsJN delays Botrytis cinerea development on grapevine inflorescences |
| title_full_unstemmed | Paraburkholderia phytofirmans PsJN delays Botrytis cinerea development on grapevine inflorescences |
| title_short | Paraburkholderia phytofirmans PsJN delays Botrytis cinerea development on grapevine inflorescences |
| title_sort | paraburkholderia phytofirmans psjn delays botrytis cinerea development on grapevine inflorescences |
| topic | Botrytis cinerea protection grapevine inflorescences antigerminative effect Paraburkholderia phytofirmans PsJN |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1030982/full |
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