Dried blood sample analysis by antibody array across the total testing process
Abstract Dried blood samples (DBSs) have many advantages; yet, impediments have limited the clinical utilization of DBSs. We developed a novel volumetric sampling device that collects a precise volume of blood, which overcomes the heterogeneity and hematocrit issues commonly encountered in a traditi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-99911-8 |
| _version_ | 1819109199735422976 |
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| author | Kelly Whittaker Ying-Qing Mao Yongping Lin Huihua Zhang Siwei Zhu Hannah Peck Ruo-Pan Huang |
| author_facet | Kelly Whittaker Ying-Qing Mao Yongping Lin Huihua Zhang Siwei Zhu Hannah Peck Ruo-Pan Huang |
| author_sort | Kelly Whittaker |
| collection | DOAJ |
| description | Abstract Dried blood samples (DBSs) have many advantages; yet, impediments have limited the clinical utilization of DBSs. We developed a novel volumetric sampling device that collects a precise volume of blood, which overcomes the heterogeneity and hematocrit issues commonly encountered in a traditional DBS card collection as well as allowing for more efficient extraction and processing procedures and thus, more efficient quantitation, by using the entire sample. We also provided a thorough procedure validation using this volumetric DBS collection device with an established quantitative proteomics analysis method, and then analyzed 1000 proteins using this approach in DBSs concomitantly with serum for future consideration of utility in clinical applications. Our data provide a first step in the establishment of a DBS database for the broad application of this sample type for widespread use in clinical proteomic and other analyses applications. |
| first_indexed | 2024-12-22T03:22:02Z |
| format | Article |
| id | doaj.art-4f8aa9dd7cfa4bd8bf5695a6656dc687 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-22T03:22:02Z |
| publishDate | 2021-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-4f8aa9dd7cfa4bd8bf5695a6656dc6872022-12-21T18:40:42ZengNature PortfolioScientific Reports2045-23222021-10-0111111310.1038/s41598-021-99911-8Dried blood sample analysis by antibody array across the total testing processKelly Whittaker0Ying-Qing Mao1Yongping Lin2Huihua Zhang3Siwei Zhu4Hannah Peck5Ruo-Pan Huang6RayBiotech LifeRayBiotech LifeDepartment of Laboratory Medicine, The First Affiliated Hospital of Guangzhou Medical UniversityRayBiotech GuangzhouRayBiotech GuangzhouRayBiotech LifeRayBiotech LifeAbstract Dried blood samples (DBSs) have many advantages; yet, impediments have limited the clinical utilization of DBSs. We developed a novel volumetric sampling device that collects a precise volume of blood, which overcomes the heterogeneity and hematocrit issues commonly encountered in a traditional DBS card collection as well as allowing for more efficient extraction and processing procedures and thus, more efficient quantitation, by using the entire sample. We also provided a thorough procedure validation using this volumetric DBS collection device with an established quantitative proteomics analysis method, and then analyzed 1000 proteins using this approach in DBSs concomitantly with serum for future consideration of utility in clinical applications. Our data provide a first step in the establishment of a DBS database for the broad application of this sample type for widespread use in clinical proteomic and other analyses applications.https://doi.org/10.1038/s41598-021-99911-8 |
| spellingShingle | Kelly Whittaker Ying-Qing Mao Yongping Lin Huihua Zhang Siwei Zhu Hannah Peck Ruo-Pan Huang Dried blood sample analysis by antibody array across the total testing process Scientific Reports |
| title | Dried blood sample analysis by antibody array across the total testing process |
| title_full | Dried blood sample analysis by antibody array across the total testing process |
| title_fullStr | Dried blood sample analysis by antibody array across the total testing process |
| title_full_unstemmed | Dried blood sample analysis by antibody array across the total testing process |
| title_short | Dried blood sample analysis by antibody array across the total testing process |
| title_sort | dried blood sample analysis by antibody array across the total testing process |
| url | https://doi.org/10.1038/s41598-021-99911-8 |
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