Immunoproteomic analysis of a Chikungunya poxvirus-based vaccine reveals high HLA class II immunoprevalence.
<h4>Background</h4>Efficient adaptive antiviral cellular and humoral immune responses require previous recognition of viral antigenic peptides bound to human leukocyte antigen (HLA) class I and II molecules, which are exposed on the surface of infected and antigen presenting cells, respe...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-07-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0007547 |
| _version_ | 1818966751145099264 |
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| author | Elena Lorente Alejandro Barriga Eilon Barnea Concepción Palomo Juan García-Arriaza Carmen Mir Mariano Esteban Arie Admon Daniel López |
| author_facet | Elena Lorente Alejandro Barriga Eilon Barnea Concepción Palomo Juan García-Arriaza Carmen Mir Mariano Esteban Arie Admon Daniel López |
| author_sort | Elena Lorente |
| collection | DOAJ |
| description | <h4>Background</h4>Efficient adaptive antiviral cellular and humoral immune responses require previous recognition of viral antigenic peptides bound to human leukocyte antigen (HLA) class I and II molecules, which are exposed on the surface of infected and antigen presenting cells, respectively. The HLA-restricted immune response to Chikungunya virus (CHIKV), a mosquito-borne Alphavirus of the Togaviridae family responsible for severe chronic polyarthralgia and polyarthritis, is largely unknown.<h4>Methodology/principal findings</h4>In this study, a high-throughput mass spectrometry analysis of complex HLA-bound peptide pools isolated from large amounts of human cells infected with a vaccinia virus (VACV) recombinant expressing CHIKV structural proteins was carried out. Twelve viral ligands from the CHIKV polyprotein naturally presented by different HLA-A, -B, and -C class I, and HLA-DR and -DP class II molecules were identified.<h4>Conclusions/significance</h4>The immunoprevalence of the HLA class II but not the HLA class I-restricted cellular immune response against the CHIKV structural polyprotein was greater than that against the VACV vector itself. In addition, most of the CHIKV HLA class I and II ligands detected by mass spectrometry are not conserved compared to its closely related O'nyong-nyong virus. These findings have clear implications for analysis of both cytotoxic and helper immune responses against CHIKV as well as for the future studies focused in the exacerbated T helper response linked to chronic musculoskeletal disorders in CHIKV patients. |
| first_indexed | 2024-12-20T13:37:52Z |
| format | Article |
| id | doaj.art-4f8bc775e33845b491b7265aab1f301a |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-20T13:37:52Z |
| publishDate | 2019-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-4f8bc775e33845b491b7265aab1f301a2022-12-21T19:38:52ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352019-07-01137e000754710.1371/journal.pntd.0007547Immunoproteomic analysis of a Chikungunya poxvirus-based vaccine reveals high HLA class II immunoprevalence.Elena LorenteAlejandro BarrigaEilon BarneaConcepción PalomoJuan García-ArriazaCarmen MirMariano EstebanArie AdmonDaniel López<h4>Background</h4>Efficient adaptive antiviral cellular and humoral immune responses require previous recognition of viral antigenic peptides bound to human leukocyte antigen (HLA) class I and II molecules, which are exposed on the surface of infected and antigen presenting cells, respectively. The HLA-restricted immune response to Chikungunya virus (CHIKV), a mosquito-borne Alphavirus of the Togaviridae family responsible for severe chronic polyarthralgia and polyarthritis, is largely unknown.<h4>Methodology/principal findings</h4>In this study, a high-throughput mass spectrometry analysis of complex HLA-bound peptide pools isolated from large amounts of human cells infected with a vaccinia virus (VACV) recombinant expressing CHIKV structural proteins was carried out. Twelve viral ligands from the CHIKV polyprotein naturally presented by different HLA-A, -B, and -C class I, and HLA-DR and -DP class II molecules were identified.<h4>Conclusions/significance</h4>The immunoprevalence of the HLA class II but not the HLA class I-restricted cellular immune response against the CHIKV structural polyprotein was greater than that against the VACV vector itself. In addition, most of the CHIKV HLA class I and II ligands detected by mass spectrometry are not conserved compared to its closely related O'nyong-nyong virus. These findings have clear implications for analysis of both cytotoxic and helper immune responses against CHIKV as well as for the future studies focused in the exacerbated T helper response linked to chronic musculoskeletal disorders in CHIKV patients.https://doi.org/10.1371/journal.pntd.0007547 |
| spellingShingle | Elena Lorente Alejandro Barriga Eilon Barnea Concepción Palomo Juan García-Arriaza Carmen Mir Mariano Esteban Arie Admon Daniel López Immunoproteomic analysis of a Chikungunya poxvirus-based vaccine reveals high HLA class II immunoprevalence. PLoS Neglected Tropical Diseases |
| title | Immunoproteomic analysis of a Chikungunya poxvirus-based vaccine reveals high HLA class II immunoprevalence. |
| title_full | Immunoproteomic analysis of a Chikungunya poxvirus-based vaccine reveals high HLA class II immunoprevalence. |
| title_fullStr | Immunoproteomic analysis of a Chikungunya poxvirus-based vaccine reveals high HLA class II immunoprevalence. |
| title_full_unstemmed | Immunoproteomic analysis of a Chikungunya poxvirus-based vaccine reveals high HLA class II immunoprevalence. |
| title_short | Immunoproteomic analysis of a Chikungunya poxvirus-based vaccine reveals high HLA class II immunoprevalence. |
| title_sort | immunoproteomic analysis of a chikungunya poxvirus based vaccine reveals high hla class ii immunoprevalence |
| url | https://doi.org/10.1371/journal.pntd.0007547 |
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