Concordance between Antimicrobial Resistance Phenotype and Genotype of <i>Staphylococcus pseudintermedius</i> from Healthy Dogs
<i>Staphylococcus pseudintermedius</i>, a common commensal canine bacterium, is the main cause of skin infections in dogs and is a potential zoonotic pathogen. The emergence of methicillin-resistant <i>S. pseudintermedius</i> (MRSP) has compromised the treatment of infections...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/11/1625 |
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| author | Joaquim Viñes Norma Fàbregas Daniel Pérez Anna Cuscó Rocío Fonticoba Olga Francino Lluís Ferrer Lourdes Migura-Garcia |
| author_facet | Joaquim Viñes Norma Fàbregas Daniel Pérez Anna Cuscó Rocío Fonticoba Olga Francino Lluís Ferrer Lourdes Migura-Garcia |
| author_sort | Joaquim Viñes |
| collection | DOAJ |
| description | <i>Staphylococcus pseudintermedius</i>, a common commensal canine bacterium, is the main cause of skin infections in dogs and is a potential zoonotic pathogen. The emergence of methicillin-resistant <i>S. pseudintermedius</i> (MRSP) has compromised the treatment of infections caused by these bacteria. In this study, we compared the phenotypic results obtained by minimum inhibitory concentration (MICs) for 67 <i>S. pseudintermedius</i> isolates from the skin of nine healthy dogs versus the genotypic data obtained with Nanopore sequencing. A total of 17 antibiotic resistance genes (ARGs) were detected among the isolates. A good correlation between phenotype and genotype was observed for some antimicrobial classes, such as ciprofloxacin (fluoroquinolone), macrolides, or tetracycline. However, for oxacillin (beta-lactam) or aminoglycosides the correlation was low. Two antibiotic resistance genes were located on plasmids integrated in the chromosome, and a third one was in a circular plasmid. To our knowledge, this is the first study assessing the correlation between phenotype and genotype regarding antimicrobial resistance of <i>S. pseudintermedius</i> from healthy dogs using Nanopore sequencing technology. |
| first_indexed | 2024-03-09T18:31:08Z |
| format | Article |
| id | doaj.art-4f8e00c4749a45a3a34bdcb210a87dea |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T18:31:08Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-4f8e00c4749a45a3a34bdcb210a87dea2023-11-24T07:30:50ZengMDPI AGAntibiotics2079-63822022-11-011111162510.3390/antibiotics11111625Concordance between Antimicrobial Resistance Phenotype and Genotype of <i>Staphylococcus pseudintermedius</i> from Healthy DogsJoaquim Viñes0Norma Fàbregas1Daniel Pérez2Anna Cuscó3Rocío Fonticoba4Olga Francino5Lluís Ferrer6Lourdes Migura-Garcia7Vetgenomics, Edifici EUREKA, PRUAB, Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, SpainVetgenomics, Edifici EUREKA, PRUAB, Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, SpainDepartment of Animal Medicine and Surgery, Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, SpainVetgenomics, Edifici EUREKA, PRUAB, Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, SpainDepartment of Animal Medicine and Surgery, Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, SpainMolecular Genetics Veterinary Service (SVGM), Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, SpainDepartment of Animal Medicine and Surgery, Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, SpainUnitat mixta d’Investigació IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Autònoma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain<i>Staphylococcus pseudintermedius</i>, a common commensal canine bacterium, is the main cause of skin infections in dogs and is a potential zoonotic pathogen. The emergence of methicillin-resistant <i>S. pseudintermedius</i> (MRSP) has compromised the treatment of infections caused by these bacteria. In this study, we compared the phenotypic results obtained by minimum inhibitory concentration (MICs) for 67 <i>S. pseudintermedius</i> isolates from the skin of nine healthy dogs versus the genotypic data obtained with Nanopore sequencing. A total of 17 antibiotic resistance genes (ARGs) were detected among the isolates. A good correlation between phenotype and genotype was observed for some antimicrobial classes, such as ciprofloxacin (fluoroquinolone), macrolides, or tetracycline. However, for oxacillin (beta-lactam) or aminoglycosides the correlation was low. Two antibiotic resistance genes were located on plasmids integrated in the chromosome, and a third one was in a circular plasmid. To our knowledge, this is the first study assessing the correlation between phenotype and genotype regarding antimicrobial resistance of <i>S. pseudintermedius</i> from healthy dogs using Nanopore sequencing technology.https://www.mdpi.com/2079-6382/11/11/1625<i>Staphylococcus pseudintermedius</i>antibiotic resistancenanopore sequencingphenotype-genotype concordancehealthy dog |
| spellingShingle | Joaquim Viñes Norma Fàbregas Daniel Pérez Anna Cuscó Rocío Fonticoba Olga Francino Lluís Ferrer Lourdes Migura-Garcia Concordance between Antimicrobial Resistance Phenotype and Genotype of <i>Staphylococcus pseudintermedius</i> from Healthy Dogs Antibiotics <i>Staphylococcus pseudintermedius</i> antibiotic resistance nanopore sequencing phenotype-genotype concordance healthy dog |
| title | Concordance between Antimicrobial Resistance Phenotype and Genotype of <i>Staphylococcus pseudintermedius</i> from Healthy Dogs |
| title_full | Concordance between Antimicrobial Resistance Phenotype and Genotype of <i>Staphylococcus pseudintermedius</i> from Healthy Dogs |
| title_fullStr | Concordance between Antimicrobial Resistance Phenotype and Genotype of <i>Staphylococcus pseudintermedius</i> from Healthy Dogs |
| title_full_unstemmed | Concordance between Antimicrobial Resistance Phenotype and Genotype of <i>Staphylococcus pseudintermedius</i> from Healthy Dogs |
| title_short | Concordance between Antimicrobial Resistance Phenotype and Genotype of <i>Staphylococcus pseudintermedius</i> from Healthy Dogs |
| title_sort | concordance between antimicrobial resistance phenotype and genotype of i staphylococcus pseudintermedius i from healthy dogs |
| topic | <i>Staphylococcus pseudintermedius</i> antibiotic resistance nanopore sequencing phenotype-genotype concordance healthy dog |
| url | https://www.mdpi.com/2079-6382/11/11/1625 |
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