RNA Nanovaccine Protects against White Spot Syndrome Virus in Shrimp
In the last 15 years, crustacean fisheries have experienced billions of dollars in economic losses, primarily due to viral diseases caused by such pathogens as white spot syndrome virus (WSSV) in the Pacific white shrimp <i>Litopenaeus vannamei</i> and Asian tiger shrimp <i>Penaeus...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/10/9/1428 |
| _version_ | 1797481620397096960 |
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| author | Yashdeep Phanse Supraja Puttamreddy Duan Loy Julia Vela Ramirez Kathleen A. Ross Ignacio Alvarez-Castro Mark Mogler Scott Broderick Krishna Rajan Balaji Narasimhan Lyric C. Bartholomay |
| author_facet | Yashdeep Phanse Supraja Puttamreddy Duan Loy Julia Vela Ramirez Kathleen A. Ross Ignacio Alvarez-Castro Mark Mogler Scott Broderick Krishna Rajan Balaji Narasimhan Lyric C. Bartholomay |
| author_sort | Yashdeep Phanse |
| collection | DOAJ |
| description | In the last 15 years, crustacean fisheries have experienced billions of dollars in economic losses, primarily due to viral diseases caused by such pathogens as white spot syndrome virus (WSSV) in the Pacific white shrimp <i>Litopenaeus vannamei</i> and Asian tiger shrimp <i>Penaeus monodon</i>. To date, no effective measures are available to prevent or control disease outbreaks in these animals, despite their economic importance. Recently, double-stranded RNA-based vaccines have been shown to provide specific and robust protection against WSSV infection in cultured shrimp. However, the limited stability of double-stranded RNA is the most significant hurdle for the field application of these vaccines with respect to delivery within an aquatic system. Polyanhydride nanoparticles have been successfully used for the encapsulation and release of vaccine antigens. We have developed a double-stranded RNA-based nanovaccine for use in shrimp disease control with emphasis on the Pacific white shrimp <i>L. vannamei</i>. Nanoparticles based on copolymers of sebacic acid, 1,6-bis(<i>p</i>-carboxyphenoxy)hexane, and 1,8-bis(<i>p</i>-carboxyphenoxy)-3,6-dioxaoctane exhibited excellent safety profiles, as measured by shrimp survival and histological evaluation. Furthermore, the nanoparticles localized to tissue target replication sites for WSSV and persisted through 28 days postadministration. Finally, the nanovaccine provided ~80% protection in a lethal WSSV challenge model. This study demonstrates the exciting potential of a safe, effective, and field-applicable RNA nanovaccine that can be rationally designed against infectious diseases affecting aquaculture. |
| first_indexed | 2024-03-09T22:17:11Z |
| format | Article |
| id | doaj.art-4f8e29c5e985486a8229ccf124c67149 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T22:17:11Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-4f8e29c5e985486a8229ccf124c671492023-11-23T19:20:59ZengMDPI AGVaccines2076-393X2022-08-01109142810.3390/vaccines10091428RNA Nanovaccine Protects against White Spot Syndrome Virus in ShrimpYashdeep Phanse0Supraja Puttamreddy1Duan Loy2Julia Vela Ramirez3Kathleen A. Ross4Ignacio Alvarez-Castro5Mark Mogler6Scott Broderick7Krishna Rajan8Balaji Narasimhan9Lyric C. Bartholomay10Department of Entomology, Iowa State University, Ames, IA 50011, USADepartment of Entomology, Iowa State University, Ames, IA 50011, USADepartment of Entomology, Iowa State University, Ames, IA 50011, USADepartment of Chemical and Biological Engineering, Nanovaccine Institute, Iowa State University, Ames, IA 50011, USADepartment of Chemical and Biological Engineering, Nanovaccine Institute, Iowa State University, Ames, IA 50011, USAInstituto de Estadística, Universidad de la República, Montevideo 11200, UruguayMerck Animal Health, Ames, IA 50010, USADepartment of Materials Science and Engineering, Iowa State University, Ames, IA 50011, USADepartment of Materials Science and Engineering, Iowa State University, Ames, IA 50011, USADepartment of Chemical and Biological Engineering, Nanovaccine Institute, Iowa State University, Ames, IA 50011, USADepartment of Entomology, Iowa State University, Ames, IA 50011, USAIn the last 15 years, crustacean fisheries have experienced billions of dollars in economic losses, primarily due to viral diseases caused by such pathogens as white spot syndrome virus (WSSV) in the Pacific white shrimp <i>Litopenaeus vannamei</i> and Asian tiger shrimp <i>Penaeus monodon</i>. To date, no effective measures are available to prevent or control disease outbreaks in these animals, despite their economic importance. Recently, double-stranded RNA-based vaccines have been shown to provide specific and robust protection against WSSV infection in cultured shrimp. However, the limited stability of double-stranded RNA is the most significant hurdle for the field application of these vaccines with respect to delivery within an aquatic system. Polyanhydride nanoparticles have been successfully used for the encapsulation and release of vaccine antigens. We have developed a double-stranded RNA-based nanovaccine for use in shrimp disease control with emphasis on the Pacific white shrimp <i>L. vannamei</i>. Nanoparticles based on copolymers of sebacic acid, 1,6-bis(<i>p</i>-carboxyphenoxy)hexane, and 1,8-bis(<i>p</i>-carboxyphenoxy)-3,6-dioxaoctane exhibited excellent safety profiles, as measured by shrimp survival and histological evaluation. Furthermore, the nanoparticles localized to tissue target replication sites for WSSV and persisted through 28 days postadministration. Finally, the nanovaccine provided ~80% protection in a lethal WSSV challenge model. This study demonstrates the exciting potential of a safe, effective, and field-applicable RNA nanovaccine that can be rationally designed against infectious diseases affecting aquaculture.https://www.mdpi.com/2076-393X/10/9/1428nanovaccinedsRNApolyanhydrideshrimpWSSV |
| spellingShingle | Yashdeep Phanse Supraja Puttamreddy Duan Loy Julia Vela Ramirez Kathleen A. Ross Ignacio Alvarez-Castro Mark Mogler Scott Broderick Krishna Rajan Balaji Narasimhan Lyric C. Bartholomay RNA Nanovaccine Protects against White Spot Syndrome Virus in Shrimp Vaccines nanovaccine dsRNA polyanhydride shrimp WSSV |
| title | RNA Nanovaccine Protects against White Spot Syndrome Virus in Shrimp |
| title_full | RNA Nanovaccine Protects against White Spot Syndrome Virus in Shrimp |
| title_fullStr | RNA Nanovaccine Protects against White Spot Syndrome Virus in Shrimp |
| title_full_unstemmed | RNA Nanovaccine Protects against White Spot Syndrome Virus in Shrimp |
| title_short | RNA Nanovaccine Protects against White Spot Syndrome Virus in Shrimp |
| title_sort | rna nanovaccine protects against white spot syndrome virus in shrimp |
| topic | nanovaccine dsRNA polyanhydride shrimp WSSV |
| url | https://www.mdpi.com/2076-393X/10/9/1428 |
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