Microbial mutagenesis by atmospheric and room-temperature plasma (ARTP): the latest development
Abstract Although rational genetic engineering is nowadays the favored method for microbial strain improvement, random mutagenesis is still in many cases the only option. Atmospheric and room-temperature plasma (ARTP) is a newly developed whole-cell mutagenesis tool based on radio-frequency atmosphe...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2018-03-01
|
| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40643-018-0200-1 |
| _version_ | 1811212809910878208 |
|---|---|
| author | Christoph Ottenheim Margarete Nawrath Jin Chuan Wu |
| author_facet | Christoph Ottenheim Margarete Nawrath Jin Chuan Wu |
| author_sort | Christoph Ottenheim |
| collection | DOAJ |
| description | Abstract Although rational genetic engineering is nowadays the favored method for microbial strain improvement, random mutagenesis is still in many cases the only option. Atmospheric and room-temperature plasma (ARTP) is a newly developed whole-cell mutagenesis tool based on radio-frequency atmospheric-pressure glow discharge plasma which features higher mutation rates than UV radiation or chemical mutagens while maintaining low treatment temperatures. It has been successfully applied on at least 24 bacterial and 14 fungal species, but also on plants, dinoflagellates, and other microbial communities for the improvement of tolerance to medium components, to increase cellular growth and production of cellular biomass, to enhance enzyme activity, and to increase the production of various chemicals. Achievements like 385.7% of acetic acid production enhancement in Acetobacter pasteurianus give this new mutagenesis tool a promising future. However, certain questions remain regarding optimal operational conditions, the effects at subcellular levels, and standard operation procedures, which need to be addressed to facilitate applications of ARTP in microbial breeding and other fields such as evolution of enzymes. |
| first_indexed | 2024-04-12T05:35:28Z |
| format | Article |
| id | doaj.art-4f8ee2405a294bae90abc7d6071d60fa |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-04-12T05:35:28Z |
| publishDate | 2018-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-4f8ee2405a294bae90abc7d6071d60fa2022-12-22T03:45:51ZengSpringerOpenBioresources and Bioprocessing2197-43652018-03-015111410.1186/s40643-018-0200-1Microbial mutagenesis by atmospheric and room-temperature plasma (ARTP): the latest developmentChristoph Ottenheim0Margarete Nawrath1Jin Chuan Wu2Institute of Chemical and Engineering Sciences, Agency for Sciences, Technology and Research (A*STAR)Institute of Chemical and Engineering Sciences, Agency for Sciences, Technology and Research (A*STAR)Institute of Chemical and Engineering Sciences, Agency for Sciences, Technology and Research (A*STAR)Abstract Although rational genetic engineering is nowadays the favored method for microbial strain improvement, random mutagenesis is still in many cases the only option. Atmospheric and room-temperature plasma (ARTP) is a newly developed whole-cell mutagenesis tool based on radio-frequency atmospheric-pressure glow discharge plasma which features higher mutation rates than UV radiation or chemical mutagens while maintaining low treatment temperatures. It has been successfully applied on at least 24 bacterial and 14 fungal species, but also on plants, dinoflagellates, and other microbial communities for the improvement of tolerance to medium components, to increase cellular growth and production of cellular biomass, to enhance enzyme activity, and to increase the production of various chemicals. Achievements like 385.7% of acetic acid production enhancement in Acetobacter pasteurianus give this new mutagenesis tool a promising future. However, certain questions remain regarding optimal operational conditions, the effects at subcellular levels, and standard operation procedures, which need to be addressed to facilitate applications of ARTP in microbial breeding and other fields such as evolution of enzymes.http://link.springer.com/article/10.1186/s40643-018-0200-1Random mutagenesisAtmospheric and room-temperature plasmaMutation mechanismStrain breedingIndustrial application |
| spellingShingle | Christoph Ottenheim Margarete Nawrath Jin Chuan Wu Microbial mutagenesis by atmospheric and room-temperature plasma (ARTP): the latest development Bioresources and Bioprocessing Random mutagenesis Atmospheric and room-temperature plasma Mutation mechanism Strain breeding Industrial application |
| title | Microbial mutagenesis by atmospheric and room-temperature plasma (ARTP): the latest development |
| title_full | Microbial mutagenesis by atmospheric and room-temperature plasma (ARTP): the latest development |
| title_fullStr | Microbial mutagenesis by atmospheric and room-temperature plasma (ARTP): the latest development |
| title_full_unstemmed | Microbial mutagenesis by atmospheric and room-temperature plasma (ARTP): the latest development |
| title_short | Microbial mutagenesis by atmospheric and room-temperature plasma (ARTP): the latest development |
| title_sort | microbial mutagenesis by atmospheric and room temperature plasma artp the latest development |
| topic | Random mutagenesis Atmospheric and room-temperature plasma Mutation mechanism Strain breeding Industrial application |
| url | http://link.springer.com/article/10.1186/s40643-018-0200-1 |
| work_keys_str_mv | AT christophottenheim microbialmutagenesisbyatmosphericandroomtemperatureplasmaartpthelatestdevelopment AT margaretenawrath microbialmutagenesisbyatmosphericandroomtemperatureplasmaartpthelatestdevelopment AT jinchuanwu microbialmutagenesisbyatmosphericandroomtemperatureplasmaartpthelatestdevelopment |