The impact of pRAP vectors on plant genetic transformation and pathogenesis studies including an analysis of BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1)-mediated resistance
Crop improvement can be facilitated through efficient gene transfer, leading to pRAP plasmid development. Comparative hairy root transformation results from 24 previously published articles examining 29,756 roots show a 70% transformation efficiency. Average gene overexpression was 11.24-fold and −3...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Journal of Plant Interactions |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/17429145.2021.1940328 |
| _version_ | 1818965919236358144 |
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| author | Vincent P. Klink Omar Darwish Nadim W. Alkharouf Katherine S. Lawrence |
| author_facet | Vincent P. Klink Omar Darwish Nadim W. Alkharouf Katherine S. Lawrence |
| author_sort | Vincent P. Klink |
| collection | DOAJ |
| description | Crop improvement can be facilitated through efficient gene transfer, leading to pRAP plasmid development. Comparative hairy root transformation results from 24 previously published articles examining 29,756 roots show a 70% transformation efficiency. Average gene overexpression was 11.24-fold and −3.84-fold in RNAi roots. New studies show Glycine max BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1) overexpression leads to a 67% decrease in Heterodera glycines parasitism while BAK1-1 RNAi led to a 4.8-fold increase in parasitism. The results show pathogen associated molecular pattern triggered immunity (PTI) functions in the G. max-H. glycines pathosystem during defense. Consequently, the pRAP vectors have applicability for studying basic biology and defense in other agricultural plants including Manihot esculenta (cassava), Zea mays (maize), Oryza sativa (rice), Triticum aestivum (wheat), Sorghum bicolor (sorghum), Brassica rapa (rape seed), Solanum tuberosum (potato), Solanum lycopersicum (tomato), Elaes guineensis (oil palm), Saccharum officinalis (sugarcane) and Beta vulgaris (sugar beet) since each have BAK1 homologs. |
| first_indexed | 2024-12-20T13:24:39Z |
| format | Article |
| id | doaj.art-4f8f40c251374f059ea3e9e2e16171fc |
| institution | Directory Open Access Journal |
| issn | 1742-9145 1742-9153 |
| language | English |
| last_indexed | 2024-12-20T13:24:39Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Plant Interactions |
| spelling | doaj.art-4f8f40c251374f059ea3e9e2e16171fc2022-12-21T19:39:18ZengTaylor & Francis GroupJournal of Plant Interactions1742-91451742-91532021-01-0116127028310.1080/17429145.2021.19403281940328The impact of pRAP vectors on plant genetic transformation and pathogenesis studies including an analysis of BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1)-mediated resistanceVincent P. Klink0Omar Darwish1Nadim W. Alkharouf2Katherine S. Lawrence3USDA-ARS-NEA-BARC Molecular Plant Pathology Laboratory, Beltsville, MD, USATexas Women’s UniversityTowson UniversityAuburn UniversityCrop improvement can be facilitated through efficient gene transfer, leading to pRAP plasmid development. Comparative hairy root transformation results from 24 previously published articles examining 29,756 roots show a 70% transformation efficiency. Average gene overexpression was 11.24-fold and −3.84-fold in RNAi roots. New studies show Glycine max BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1) overexpression leads to a 67% decrease in Heterodera glycines parasitism while BAK1-1 RNAi led to a 4.8-fold increase in parasitism. The results show pathogen associated molecular pattern triggered immunity (PTI) functions in the G. max-H. glycines pathosystem during defense. Consequently, the pRAP vectors have applicability for studying basic biology and defense in other agricultural plants including Manihot esculenta (cassava), Zea mays (maize), Oryza sativa (rice), Triticum aestivum (wheat), Sorghum bicolor (sorghum), Brassica rapa (rape seed), Solanum tuberosum (potato), Solanum lycopersicum (tomato), Elaes guineensis (oil palm), Saccharum officinalis (sugarcane) and Beta vulgaris (sugar beet) since each have BAK1 homologs.http://dx.doi.org/10.1080/17429145.2021.1940328planttransformationplasmidprap15prap17 overexpressionheterologous expressionectopic expressionrna interference (rnai)cropgeneticengineeringnematodebri1-associated receptor kinase 1bak1 |
| spellingShingle | Vincent P. Klink Omar Darwish Nadim W. Alkharouf Katherine S. Lawrence The impact of pRAP vectors on plant genetic transformation and pathogenesis studies including an analysis of BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1)-mediated resistance Journal of Plant Interactions plant transformation plasmid prap15 prap17 overexpression heterologous expression ectopic expression rna interference (rnai) crop genetic engineering nematode bri1-associated receptor kinase 1 bak1 |
| title | The impact of pRAP vectors on plant genetic transformation and pathogenesis studies including an analysis of BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1)-mediated resistance |
| title_full | The impact of pRAP vectors on plant genetic transformation and pathogenesis studies including an analysis of BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1)-mediated resistance |
| title_fullStr | The impact of pRAP vectors on plant genetic transformation and pathogenesis studies including an analysis of BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1)-mediated resistance |
| title_full_unstemmed | The impact of pRAP vectors on plant genetic transformation and pathogenesis studies including an analysis of BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1)-mediated resistance |
| title_short | The impact of pRAP vectors on plant genetic transformation and pathogenesis studies including an analysis of BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1)-mediated resistance |
| title_sort | impact of prap vectors on plant genetic transformation and pathogenesis studies including an analysis of bri1 associated receptor kinase 1 bak1 mediated resistance |
| topic | plant transformation plasmid prap15 prap17 overexpression heterologous expression ectopic expression rna interference (rnai) crop genetic engineering nematode bri1-associated receptor kinase 1 bak1 |
| url | http://dx.doi.org/10.1080/17429145.2021.1940328 |
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