Should liquid based cytology (LBC) be applied to thyroid fine needle aspiration cytology samples?: Comparative analysis of conventional and LBC smears
Context: Liquid-based cytology (LBC) is a cyto-preparatory technique that may be applied to fine-needle aspiration (FNA) samples. However, its efficacy over conventional smears (CS) in thyroid is controversial. Aims: The aim of this study was to compare CS versus LBC (SurePath, BD) preparations in t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | Journal of Cytology |
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| Online Access: | http://www.jcytol.org/article.asp?issn=0970-9371;year=2021;volume=38;issue=4;spage=198;epage=202;aulast=Mahajan |
| _version_ | 1828131040321863680 |
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| author | Swati Mahajan Arvind Rajwanshi Radhika Srinivasan Bishan Dass Radotra Naresh Panda |
| author_facet | Swati Mahajan Arvind Rajwanshi Radhika Srinivasan Bishan Dass Radotra Naresh Panda |
| author_sort | Swati Mahajan |
| collection | DOAJ |
| description | Context: Liquid-based cytology (LBC) is a cyto-preparatory technique that may be applied to fine-needle aspiration (FNA) samples. However, its efficacy over conventional smears (CS) in thyroid is controversial. Aims: The aim of this study was to compare CS versus LBC (SurePath, BD) preparations in thyroid FNA samples for diagnostic efficacy. Settings and Design: Prospective case-control study using split sample analysis in 200 non-consecutive cases of thyroid FNA reported as per the Bethesda system. Methods and Material: Detailed cytomorphological features were evaluated in CS and LBC preparations. Cellularity was scored as 0–3. Based on diagnostic efficacy, they were categorized into three groups:(i) CS and LBC equivalent for diagnosis, (ii) CS better than LBC, and (iii) LBC better than CS for providing the diagnosis. Statistical Analysis Used: Paired t test for cellularity scores and descriptive for diagnostic efficacy. Results: There were 7 unsatisfactory, 118 benign, 10 atypia or follicular lesion of undetermined significance, 25 follicular neoplasms, 6 suspicious for malignancy, and 34 malignant thyroid FNA cases based on routine conventional smears. Cellularity of conventional smears was significantly higher than paired LBC smears (paired t test, P < 0.005). Comparison of overall diagnostic efficacy showed that LBC and CS were equivalent in 59% of cases; CS was superior to LBC in 37% cases and LBC was better than CS in 4% cases only. LBC smears showed higher unsatisfactory rate as compared to CS (18% vs. 3.5%). In category 2, the two techniques were equivalent in 87% of cases. In categories 4 and 6, CS were superior to LBC. Conclusions: In thyroid FNA cases, conventional smears are superior to LBC preparation in terms of cellularity and diagnostic efficacy and hence, LBC preparations should not replace conventional smears for making a routine cytomorphological diagnosis. |
| first_indexed | 2024-04-11T16:41:28Z |
| format | Article |
| id | doaj.art-4f928a5dba9c43cc8ffbd659f3f3cea7 |
| institution | Directory Open Access Journal |
| issn | 0970-9371 |
| language | English |
| last_indexed | 2024-04-11T16:41:28Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Cytology |
| spelling | doaj.art-4f928a5dba9c43cc8ffbd659f3f3cea72022-12-22T04:13:40ZengWolters Kluwer Medknow PublicationsJournal of Cytology0970-93712021-01-0138419820210.4103/JOC.JOC_6_17Should liquid based cytology (LBC) be applied to thyroid fine needle aspiration cytology samples?: Comparative analysis of conventional and LBC smearsSwati MahajanArvind RajwanshiRadhika SrinivasanBishan Dass RadotraNaresh PandaContext: Liquid-based cytology (LBC) is a cyto-preparatory technique that may be applied to fine-needle aspiration (FNA) samples. However, its efficacy over conventional smears (CS) in thyroid is controversial. Aims: The aim of this study was to compare CS versus LBC (SurePath, BD) preparations in thyroid FNA samples for diagnostic efficacy. Settings and Design: Prospective case-control study using split sample analysis in 200 non-consecutive cases of thyroid FNA reported as per the Bethesda system. Methods and Material: Detailed cytomorphological features were evaluated in CS and LBC preparations. Cellularity was scored as 0–3. Based on diagnostic efficacy, they were categorized into three groups:(i) CS and LBC equivalent for diagnosis, (ii) CS better than LBC, and (iii) LBC better than CS for providing the diagnosis. Statistical Analysis Used: Paired t test for cellularity scores and descriptive for diagnostic efficacy. Results: There were 7 unsatisfactory, 118 benign, 10 atypia or follicular lesion of undetermined significance, 25 follicular neoplasms, 6 suspicious for malignancy, and 34 malignant thyroid FNA cases based on routine conventional smears. Cellularity of conventional smears was significantly higher than paired LBC smears (paired t test, P < 0.005). Comparison of overall diagnostic efficacy showed that LBC and CS were equivalent in 59% of cases; CS was superior to LBC in 37% cases and LBC was better than CS in 4% cases only. LBC smears showed higher unsatisfactory rate as compared to CS (18% vs. 3.5%). In category 2, the two techniques were equivalent in 87% of cases. In categories 4 and 6, CS were superior to LBC. Conclusions: In thyroid FNA cases, conventional smears are superior to LBC preparation in terms of cellularity and diagnostic efficacy and hence, LBC preparations should not replace conventional smears for making a routine cytomorphological diagnosis.http://www.jcytol.org/article.asp?issn=0970-9371;year=2021;volume=38;issue=4;spage=198;epage=202;aulast=Mahajanconventional smearefficacyfine-needle aspirationliquid-based cytologythyroid |
| spellingShingle | Swati Mahajan Arvind Rajwanshi Radhika Srinivasan Bishan Dass Radotra Naresh Panda Should liquid based cytology (LBC) be applied to thyroid fine needle aspiration cytology samples?: Comparative analysis of conventional and LBC smears Journal of Cytology conventional smear efficacy fine-needle aspiration liquid-based cytology thyroid |
| title | Should liquid based cytology (LBC) be applied to thyroid fine needle aspiration cytology samples?: Comparative analysis of conventional and LBC smears |
| title_full | Should liquid based cytology (LBC) be applied to thyroid fine needle aspiration cytology samples?: Comparative analysis of conventional and LBC smears |
| title_fullStr | Should liquid based cytology (LBC) be applied to thyroid fine needle aspiration cytology samples?: Comparative analysis of conventional and LBC smears |
| title_full_unstemmed | Should liquid based cytology (LBC) be applied to thyroid fine needle aspiration cytology samples?: Comparative analysis of conventional and LBC smears |
| title_short | Should liquid based cytology (LBC) be applied to thyroid fine needle aspiration cytology samples?: Comparative analysis of conventional and LBC smears |
| title_sort | should liquid based cytology lbc be applied to thyroid fine needle aspiration cytology samples comparative analysis of conventional and lbc smears |
| topic | conventional smear efficacy fine-needle aspiration liquid-based cytology thyroid |
| url | http://www.jcytol.org/article.asp?issn=0970-9371;year=2021;volume=38;issue=4;spage=198;epage=202;aulast=Mahajan |
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