Molecular systems for the characterization of fungi of the genus Aspergillus

Taxonomy is generally considered a synonym of systematics and is traditionally divided into classification, nomenclature and identification, the process of determining whether an unknown organism belongs to one of the units defined. The new taxonomies are based on a polyphasic approach using phenoty...

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Библиографические подробности
Главный автор: Stefano Andreoni
Формат: Статья
Язык:English
Опубликовано: PAGEPress Publications 2011-12-01
Серии:Microbiologia Medica
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Online-ссылка:http://www.pagepressjournals.org/index.php/mm/article/view/2337
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author Stefano Andreoni
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author_sort Stefano Andreoni
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description Taxonomy is generally considered a synonym of systematics and is traditionally divided into classification, nomenclature and identification, the process of determining whether an unknown organism belongs to one of the units defined. The new taxonomies are based on a polyphasic approach using phenotypical characters together with multigene DNA sequences. The term “polyphasic” taxonomy described the integration of all available genotypic, phenotypic, and phylogenetic information into a consensus type of classification. These information can be used to classify fungi belong to the genus Aspergillus. Over the years, many different molecular methods have been developed for Aspergillus strain typing and to investigate the epidemiological relation between environmental and clinical isolates. Several typing methods have been described for Aspergillus species: restriction fragment lenght polymorphisms (RFLP), Random Amplification Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP), Multilocus sequenze typing (MLST) and Random amplified microsatellites (RAMS). The advantages and disavantages of the different typing techniques are evaluated in terms of their discriminatory power, reproducibility and exchangeability, applicability, easy of use, and interpretation.
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spelling doaj.art-4f932d4d1b3f4556a93c26d55b8aaacd2022-12-22T03:06:37ZengPAGEPress PublicationsMicrobiologia Medica2280-64232011-12-0126410.4081/mm.2011.23371591Molecular systems for the characterization of fungi of the genus AspergillusStefano AndreoniTaxonomy is generally considered a synonym of systematics and is traditionally divided into classification, nomenclature and identification, the process of determining whether an unknown organism belongs to one of the units defined. The new taxonomies are based on a polyphasic approach using phenotypical characters together with multigene DNA sequences. The term “polyphasic” taxonomy described the integration of all available genotypic, phenotypic, and phylogenetic information into a consensus type of classification. These information can be used to classify fungi belong to the genus Aspergillus. Over the years, many different molecular methods have been developed for Aspergillus strain typing and to investigate the epidemiological relation between environmental and clinical isolates. Several typing methods have been described for Aspergillus species: restriction fragment lenght polymorphisms (RFLP), Random Amplification Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP), Multilocus sequenze typing (MLST) and Random amplified microsatellites (RAMS). The advantages and disavantages of the different typing techniques are evaluated in terms of their discriminatory power, reproducibility and exchangeability, applicability, easy of use, and interpretation.http://www.pagepressjournals.org/index.php/mm/article/view/2337Aspergillus, characterization, taxonomy, genotyping
spellingShingle Stefano Andreoni
Molecular systems for the characterization of fungi of the genus Aspergillus
Microbiologia Medica
Aspergillus, characterization, taxonomy, genotyping
title Molecular systems for the characterization of fungi of the genus Aspergillus
title_full Molecular systems for the characterization of fungi of the genus Aspergillus
title_fullStr Molecular systems for the characterization of fungi of the genus Aspergillus
title_full_unstemmed Molecular systems for the characterization of fungi of the genus Aspergillus
title_short Molecular systems for the characterization of fungi of the genus Aspergillus
title_sort molecular systems for the characterization of fungi of the genus aspergillus
topic Aspergillus, characterization, taxonomy, genotyping
url http://www.pagepressjournals.org/index.php/mm/article/view/2337
work_keys_str_mv AT stefanoandreoni molecularsystemsforthecharacterizationoffungiofthegenusaspergillus