Influence of the genotype and density of inoculation on the differentiation of somatic embryos of Coffea arabica L. cv. Red Caturra and Coffea canephora cv. Robusta
The conditions were established for the differentiation of somatic embryos from cell suspensions in the genotype Caturra rojo (Coffea arabica) and Robusta (Coffea canephora). Cell suspensions with high embryogenic potentials and stable coefficients of multiplication were used. While studying the den...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidad Central Marta Abreu de Las Villas
2003-07-01
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| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/242 |
| Summary: | The conditions were established for the differentiation of somatic embryos from cell suspensions in the genotype Caturra rojo (Coffea arabica) and Robusta (Coffea canephora). Cell suspensions with high embryogenic potentials and stable coefficients of multiplication were used. While studying the density of inoculation, for the phase of differentiation for both varieties, differences appeared in the embryogenic capacity among them, being reached a whole of 556 500 ES.l-1 for the variety Caturra rojo and 298 670 SE.l-1 for the variety Robusta. The biggest number of embryos in torpedo state, were obtained with a density of inoculation of 0.5 gFW.l-1 for the variety Caturra rojo and 5.0 gMF.l-1 for the variety Robusta.
Key Words: cell suspensions, embryogenic potential, somatic Embryogenesis, embryogenic cells |
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| ISSN: | 1609-1841 2074-8647 |