Lung Cancer Cell-Derived Exosome Detection Using Electrochemical Approach towards Early Cancer Screening
Lung cancer is one of the deadliest cancers worldwide due to the inability of existing methods for early diagnosis. Tumor-derived exosomes are nano-scale vesicles released from tumor cells to the extracellular environment, and their investigation can be very useful in both biomarkers for early cance...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/24/17225 |
| _version_ | 1797380760149164032 |
|---|---|
| author | Koosha Irani Hossein Siampour Abdollah Allahverdi Ahmad Moshaii Hossein Naderi-Manesh |
| author_facet | Koosha Irani Hossein Siampour Abdollah Allahverdi Ahmad Moshaii Hossein Naderi-Manesh |
| author_sort | Koosha Irani |
| collection | DOAJ |
| description | Lung cancer is one of the deadliest cancers worldwide due to the inability of existing methods for early diagnosis. Tumor-derived exosomes are nano-scale vesicles released from tumor cells to the extracellular environment, and their investigation can be very useful in both biomarkers for early cancer screening and treatment assessment. This research detected the exosomes via an ultrasensitive electrochemical biosensor containing gold nano-islands (Au-NIs) structures. This way, a high surface-area-to-volume ratio of nanostructures was embellished on the FTO electrodes to increase the chance of immobilizing the CD-151 antibody. In this way, a layer of gold was first deposited on the electrode by physical vapor deposition (PVD), followed by thermal annealing to construct primary gold seeds on the surface of the electrode. Then, gold seeds were grown by electrochemical deposition through gold salt. The cell-derived exosomes were successfully immobilized on the FTO electrode through the CD-151 antibody, and cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods were used in this research. In the CV method, the change in the current passing through the working electrode is measured so that the connection of exosomes causes the current to decrease. In the EIS method, surface resistance changes were investigated so that the binding of exosomes increased the surface resistance. Various concentrations of exosomes in both cell culture and blood serum samples were measured to test the sensitivity of the biosensor, which makes our biosensor capable of detecting 20 exosomes per milliliter. |
| first_indexed | 2024-03-08T20:41:43Z |
| format | Article |
| id | doaj.art-4f981df3c1994a3d939f062a3f7d6dc1 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T20:41:43Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4f981df3c1994a3d939f062a3f7d6dc12023-12-22T14:13:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0124241722510.3390/ijms242417225Lung Cancer Cell-Derived Exosome Detection Using Electrochemical Approach towards Early Cancer ScreeningKoosha Irani0Hossein Siampour1Abdollah Allahverdi2Ahmad Moshaii3Hossein Naderi-Manesh4Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran P.O. Box 14115-154, IranBiosensor Research Center (BRC), Isfahan University of Medical Sciences, Isfahan P.O. Box 81746-73461, IranDepartment of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran P.O. Box 14115-154, IranDepartment of Physics, Tarbiat Modares University, Tehran P.O. Box 14115-175, IranDepartment of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran P.O. Box 14115-154, IranLung cancer is one of the deadliest cancers worldwide due to the inability of existing methods for early diagnosis. Tumor-derived exosomes are nano-scale vesicles released from tumor cells to the extracellular environment, and their investigation can be very useful in both biomarkers for early cancer screening and treatment assessment. This research detected the exosomes via an ultrasensitive electrochemical biosensor containing gold nano-islands (Au-NIs) structures. This way, a high surface-area-to-volume ratio of nanostructures was embellished on the FTO electrodes to increase the chance of immobilizing the CD-151 antibody. In this way, a layer of gold was first deposited on the electrode by physical vapor deposition (PVD), followed by thermal annealing to construct primary gold seeds on the surface of the electrode. Then, gold seeds were grown by electrochemical deposition through gold salt. The cell-derived exosomes were successfully immobilized on the FTO electrode through the CD-151 antibody, and cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods were used in this research. In the CV method, the change in the current passing through the working electrode is measured so that the connection of exosomes causes the current to decrease. In the EIS method, surface resistance changes were investigated so that the binding of exosomes increased the surface resistance. Various concentrations of exosomes in both cell culture and blood serum samples were measured to test the sensitivity of the biosensor, which makes our biosensor capable of detecting 20 exosomes per milliliter.https://www.mdpi.com/1422-0067/24/24/17225exosomesearly lung cancer diagnosiselectrochemical biosensorA549 cell line |
| spellingShingle | Koosha Irani Hossein Siampour Abdollah Allahverdi Ahmad Moshaii Hossein Naderi-Manesh Lung Cancer Cell-Derived Exosome Detection Using Electrochemical Approach towards Early Cancer Screening International Journal of Molecular Sciences exosomes early lung cancer diagnosis electrochemical biosensor A549 cell line |
| title | Lung Cancer Cell-Derived Exosome Detection Using Electrochemical Approach towards Early Cancer Screening |
| title_full | Lung Cancer Cell-Derived Exosome Detection Using Electrochemical Approach towards Early Cancer Screening |
| title_fullStr | Lung Cancer Cell-Derived Exosome Detection Using Electrochemical Approach towards Early Cancer Screening |
| title_full_unstemmed | Lung Cancer Cell-Derived Exosome Detection Using Electrochemical Approach towards Early Cancer Screening |
| title_short | Lung Cancer Cell-Derived Exosome Detection Using Electrochemical Approach towards Early Cancer Screening |
| title_sort | lung cancer cell derived exosome detection using electrochemical approach towards early cancer screening |
| topic | exosomes early lung cancer diagnosis electrochemical biosensor A549 cell line |
| url | https://www.mdpi.com/1422-0067/24/24/17225 |
| work_keys_str_mv | AT kooshairani lungcancercellderivedexosomedetectionusingelectrochemicalapproachtowardsearlycancerscreening AT hosseinsiampour lungcancercellderivedexosomedetectionusingelectrochemicalapproachtowardsearlycancerscreening AT abdollahallahverdi lungcancercellderivedexosomedetectionusingelectrochemicalapproachtowardsearlycancerscreening AT ahmadmoshaii lungcancercellderivedexosomedetectionusingelectrochemicalapproachtowardsearlycancerscreening AT hosseinnaderimanesh lungcancercellderivedexosomedetectionusingelectrochemicalapproachtowardsearlycancerscreening |