Effects of concentrated growth factors on the angiogenic properties of dental pulp cells and endothelial cells: an in vitro study
Abstract The aim was to investigate the angiogenic effects of concentrated growth factors on human dental pulp cells and human umbilical vein endothelial cells. Cells were treated with concentrated growth factor extracts. The CCK-8 assay and cell cycle assay were conducted to evaluate cell growth. C...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pesquisa Odontológica
2018-06-01
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| Series: | Brazilian Oral Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100244&lng=en&tlng=en |
| _version_ | 1818050642539708416 |
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| author | Huan JUN Dou LEI Yan QIFANG Xu YUAN Yang DEQIN |
| author_facet | Huan JUN Dou LEI Yan QIFANG Xu YUAN Yang DEQIN |
| author_sort | Huan JUN |
| collection | DOAJ |
| description | Abstract The aim was to investigate the angiogenic effects of concentrated growth factors on human dental pulp cells and human umbilical vein endothelial cells. Cells were treated with concentrated growth factor extracts. The CCK-8 assay and cell cycle assay were conducted to evaluate cell growth. Cell migration was evaluated by the Transwell migration assay. Angiogenesis-associated mRNA and protein expression levels were determined using quantitative real-time PCR and Western blotting, respectively. A tube formation assay was conducted to evaluate the angiogenic capacity in vitro. The data showed that compared with the control, concentrated growth factor extracts significantly promoted dental pulp cell proliferation and differentiation and endothelial cell proliferation and migration in a dose-dependent manner (p < 0.05). Concentrated growth factor extracts also promoted the tube-like structure formation of endothelial cells in vitro. The RT-PCR and Western blot results showed that concentrated growth factor extracts upregulated the expression of angiogenesis-related genes – chemokine receptor-4, platelet-derived growth factor, and vascular endothelial growth factor – in dental pulp cells. In conclusion, concentrated growth factors showed proangiogenic effects on dental pulp cells and endothelial cells and have good application potential for dental pulp revascularization. |
| first_indexed | 2024-12-10T10:56:43Z |
| format | Article |
| id | doaj.art-4f98471b0662436991a93d9e8cac741f |
| institution | Directory Open Access Journal |
| issn | 1807-3107 |
| language | English |
| last_indexed | 2024-12-10T10:56:43Z |
| publishDate | 2018-06-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-4f98471b0662436991a93d9e8cac741f2022-12-22T01:51:49ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31072018-06-0132010.1590/1807-3107bor-2018.vol32.0048S1806-83242018000100244Effects of concentrated growth factors on the angiogenic properties of dental pulp cells and endothelial cells: an in vitro studyHuan JUNDou LEIYan QIFANGXu YUANYang DEQINAbstract The aim was to investigate the angiogenic effects of concentrated growth factors on human dental pulp cells and human umbilical vein endothelial cells. Cells were treated with concentrated growth factor extracts. The CCK-8 assay and cell cycle assay were conducted to evaluate cell growth. Cell migration was evaluated by the Transwell migration assay. Angiogenesis-associated mRNA and protein expression levels were determined using quantitative real-time PCR and Western blotting, respectively. A tube formation assay was conducted to evaluate the angiogenic capacity in vitro. The data showed that compared with the control, concentrated growth factor extracts significantly promoted dental pulp cell proliferation and differentiation and endothelial cell proliferation and migration in a dose-dependent manner (p < 0.05). Concentrated growth factor extracts also promoted the tube-like structure formation of endothelial cells in vitro. The RT-PCR and Western blot results showed that concentrated growth factor extracts upregulated the expression of angiogenesis-related genes – chemokine receptor-4, platelet-derived growth factor, and vascular endothelial growth factor – in dental pulp cells. In conclusion, concentrated growth factors showed proangiogenic effects on dental pulp cells and endothelial cells and have good application potential for dental pulp revascularization.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100244&lng=en&tlng=enEndothelial CellsEndodontics |
| spellingShingle | Huan JUN Dou LEI Yan QIFANG Xu YUAN Yang DEQIN Effects of concentrated growth factors on the angiogenic properties of dental pulp cells and endothelial cells: an in vitro study Brazilian Oral Research Endothelial Cells Endodontics |
| title | Effects of concentrated growth factors on the angiogenic properties of dental pulp cells and endothelial cells: an in vitro study |
| title_full | Effects of concentrated growth factors on the angiogenic properties of dental pulp cells and endothelial cells: an in vitro study |
| title_fullStr | Effects of concentrated growth factors on the angiogenic properties of dental pulp cells and endothelial cells: an in vitro study |
| title_full_unstemmed | Effects of concentrated growth factors on the angiogenic properties of dental pulp cells and endothelial cells: an in vitro study |
| title_short | Effects of concentrated growth factors on the angiogenic properties of dental pulp cells and endothelial cells: an in vitro study |
| title_sort | effects of concentrated growth factors on the angiogenic properties of dental pulp cells and endothelial cells an in vitro study |
| topic | Endothelial Cells Endodontics |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100244&lng=en&tlng=en |
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