Multivalent Sialyllactose-Levan-Conjugated Gold Nanoparticles for Efficient Interaction with and Colorimetric Detection of Influenza A Virus
We report a colorimetric assay to detect influenza A virus using sialyllactose-levan-conjugated gold nanoparticles (AuNPs). We successfully conjugated 2, 3- and 2, 6-sialyllactose to levan and synthesized sialyllactose-levan-conjugated AuNPs. Each sialyllactose-conjugated levan specifically interact...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/9/7/186 |
| _version_ | 1797527379376078848 |
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| author | Sun-Jung Kim Pan Kee Bae Yong-Beom Shin |
| author_facet | Sun-Jung Kim Pan Kee Bae Yong-Beom Shin |
| author_sort | Sun-Jung Kim |
| collection | DOAJ |
| description | We report a colorimetric assay to detect influenza A virus using sialyllactose-levan-conjugated gold nanoparticles (AuNPs). We successfully conjugated 2, 3- and 2, 6-sialyllactose to levan and synthesized sialyllactose-levan-conjugated AuNPs. Each sialyllactose-conjugated levan specifically interacted with a recognizable lectin. Synthesized sialyllactose-conjugated levan acted as reducing and coating agents during the formation of AuNPs. Human influenza A virus specifically bound to 2, 6-sialyllactose-levan-conjugated AuNPs. Moreover, 2, 6-sialyllactose-conjugated levan AuNPs rapidly changed color from red to blue after incubation with human influenza virus. For detecting avian influenza virus, 2, 3-sialyllactose-levan-conjugated AuNPs were more effective than 2, 6-sialyllactose-levan-conjugated AuNPs. Therefore, the efficient targeting and diagnosis of influenza virus according to origin was possible. The deployment of sialyllactose-levan-conjugated particles for the detection of influenza virus is simple and quick. The limit of detection (L.O.D) of H1N1 influenza virus was 7.4 × 10<sup>3</sup> pfu using 2, 6-siallylactose-levan-conjugated AuNPs and H5N2 influenza virus was 4.2 × 10<sup>3</sup> pfu using 2, 3-siallylactose-levan- conjugated AuNPs. |
| first_indexed | 2024-03-10T09:42:03Z |
| format | Article |
| id | doaj.art-4f99e86aed41457c9963e3452fff559f |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-10T09:42:03Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-4f99e86aed41457c9963e3452fff559f2023-11-22T03:31:26ZengMDPI AGChemosensors2227-90402021-07-019718610.3390/chemosensors9070186Multivalent Sialyllactose-Levan-Conjugated Gold Nanoparticles for Efficient Interaction with and Colorimetric Detection of Influenza A VirusSun-Jung Kim0Pan Kee Bae1Yong-Beom Shin2BioNano Health Guard Research Center, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, KoreaBioNano Health Guard Research Center, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, KoreaBioNano Health Guard Research Center, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, KoreaWe report a colorimetric assay to detect influenza A virus using sialyllactose-levan-conjugated gold nanoparticles (AuNPs). We successfully conjugated 2, 3- and 2, 6-sialyllactose to levan and synthesized sialyllactose-levan-conjugated AuNPs. Each sialyllactose-conjugated levan specifically interacted with a recognizable lectin. Synthesized sialyllactose-conjugated levan acted as reducing and coating agents during the formation of AuNPs. Human influenza A virus specifically bound to 2, 6-sialyllactose-levan-conjugated AuNPs. Moreover, 2, 6-sialyllactose-conjugated levan AuNPs rapidly changed color from red to blue after incubation with human influenza virus. For detecting avian influenza virus, 2, 3-sialyllactose-levan-conjugated AuNPs were more effective than 2, 6-sialyllactose-levan-conjugated AuNPs. Therefore, the efficient targeting and diagnosis of influenza virus according to origin was possible. The deployment of sialyllactose-levan-conjugated particles for the detection of influenza virus is simple and quick. The limit of detection (L.O.D) of H1N1 influenza virus was 7.4 × 10<sup>3</sup> pfu using 2, 6-siallylactose-levan-conjugated AuNPs and H5N2 influenza virus was 4.2 × 10<sup>3</sup> pfu using 2, 3-siallylactose-levan- conjugated AuNPs.https://www.mdpi.com/2227-9040/9/7/186levansialyllactosegold nanoparticlecolorimetric assayinfluenza |
| spellingShingle | Sun-Jung Kim Pan Kee Bae Yong-Beom Shin Multivalent Sialyllactose-Levan-Conjugated Gold Nanoparticles for Efficient Interaction with and Colorimetric Detection of Influenza A Virus Chemosensors levan sialyllactose gold nanoparticle colorimetric assay influenza |
| title | Multivalent Sialyllactose-Levan-Conjugated Gold Nanoparticles for Efficient Interaction with and Colorimetric Detection of Influenza A Virus |
| title_full | Multivalent Sialyllactose-Levan-Conjugated Gold Nanoparticles for Efficient Interaction with and Colorimetric Detection of Influenza A Virus |
| title_fullStr | Multivalent Sialyllactose-Levan-Conjugated Gold Nanoparticles for Efficient Interaction with and Colorimetric Detection of Influenza A Virus |
| title_full_unstemmed | Multivalent Sialyllactose-Levan-Conjugated Gold Nanoparticles for Efficient Interaction with and Colorimetric Detection of Influenza A Virus |
| title_short | Multivalent Sialyllactose-Levan-Conjugated Gold Nanoparticles for Efficient Interaction with and Colorimetric Detection of Influenza A Virus |
| title_sort | multivalent sialyllactose levan conjugated gold nanoparticles for efficient interaction with and colorimetric detection of influenza a virus |
| topic | levan sialyllactose gold nanoparticle colorimetric assay influenza |
| url | https://www.mdpi.com/2227-9040/9/7/186 |
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