Peptide-based siRNA delivery system for tumor vascular normalization and gene silencing in 4T1 cells
Summary: Efficient gene delivery in an integrated drug delivery system is urgent for multimodal antitumor therapy. Herein, we describe a protocol for constructing a peptide-based siRNA delivery system to achieve tumor vascular normalization and gene silencing in 4T1 cells. We highlighted four major...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723000965 |
| _version_ | 1811161687298932736 |
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| author | Yunfei Yi Chan Feng Mian Yu Lin Mei Meiying Wu Wei Tao |
| author_facet | Yunfei Yi Chan Feng Mian Yu Lin Mei Meiying Wu Wei Tao |
| author_sort | Yunfei Yi |
| collection | DOAJ |
| description | Summary: Efficient gene delivery in an integrated drug delivery system is urgent for multimodal antitumor therapy. Herein, we describe a protocol for constructing a peptide-based siRNA delivery system to achieve tumor vascular normalization and gene silencing in 4T1 cells. We highlighted four major steps, including (1) synthesis of the chimeric peptide, (2) preparation and characterization of PA7R@siRNA micelleplexes, (3) in vitro tube formation assay and transwell cell migration assay, and (4) siRNA transfection in 4T1 cells. This delivery system is expected to be used to silence gene expression, normalize tumor vasculature, and perform other treatments based on the different peptide segments.For complete details on the use and execution of this protocol, please refer to Yi et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-10T06:18:22Z |
| format | Article |
| id | doaj.art-4f9ed319407445c590ae8e477e7216d7 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-10T06:18:22Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-4f9ed319407445c590ae8e477e7216d72023-03-02T05:03:06ZengElsevierSTAR Protocols2666-16672023-03-0141102138Peptide-based siRNA delivery system for tumor vascular normalization and gene silencing in 4T1 cellsYunfei Yi0Chan Feng1Mian Yu2Lin Mei3Meiying Wu4Wei Tao5School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, ChinaCenter for Nanomedicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02115, USA; Department of Respiratory Medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310016, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, China; Tianjin Key Laboratory of Biomedical Materials, Key Laboratory of Biomaterials and Nanotechnology for Cancer Immunotherapy, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, ChinaSchool of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, China; Corresponding authorCenter for Nanomedicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02115, USA; Corresponding authorSummary: Efficient gene delivery in an integrated drug delivery system is urgent for multimodal antitumor therapy. Herein, we describe a protocol for constructing a peptide-based siRNA delivery system to achieve tumor vascular normalization and gene silencing in 4T1 cells. We highlighted four major steps, including (1) synthesis of the chimeric peptide, (2) preparation and characterization of PA7R@siRNA micelleplexes, (3) in vitro tube formation assay and transwell cell migration assay, and (4) siRNA transfection in 4T1 cells. This delivery system is expected to be used to silence gene expression, normalize tumor vasculature, and perform other treatments based on the different peptide segments.For complete details on the use and execution of this protocol, please refer to Yi et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723000965CancerHealth SciencesMolecular BiologyBiotechnology and BioengineeringChemistry |
| spellingShingle | Yunfei Yi Chan Feng Mian Yu Lin Mei Meiying Wu Wei Tao Peptide-based siRNA delivery system for tumor vascular normalization and gene silencing in 4T1 cells STAR Protocols Cancer Health Sciences Molecular Biology Biotechnology and Bioengineering Chemistry |
| title | Peptide-based siRNA delivery system for tumor vascular normalization and gene silencing in 4T1 cells |
| title_full | Peptide-based siRNA delivery system for tumor vascular normalization and gene silencing in 4T1 cells |
| title_fullStr | Peptide-based siRNA delivery system for tumor vascular normalization and gene silencing in 4T1 cells |
| title_full_unstemmed | Peptide-based siRNA delivery system for tumor vascular normalization and gene silencing in 4T1 cells |
| title_short | Peptide-based siRNA delivery system for tumor vascular normalization and gene silencing in 4T1 cells |
| title_sort | peptide based sirna delivery system for tumor vascular normalization and gene silencing in 4t1 cells |
| topic | Cancer Health Sciences Molecular Biology Biotechnology and Bioengineering Chemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723000965 |
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