An inorganic mineral-based protocell with prebiotic radiation fitness
Abstract Protocell fitness under extreme prebiotic conditions is critical in understanding the origin of life. However, little is known about protocell’s survival and fitness under prebiotic radiations. Here we present a radioresistant protocell model based on assembly of two types of coacervate dro...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-12-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-43272-5 |
| _version_ | 1797397871492857856 |
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| author | Shang Dai Zhenming Xie Binqiang Wang Rui Ye Xinwen Ou Chen Wang Ning Yu Cheng Huang Jie Zhao Chunhui Cai Furong Zhang Damiano Buratto Taimoor Khan Yan Qiao Yuejin Hua Ruhong Zhou Bing Tian |
| author_facet | Shang Dai Zhenming Xie Binqiang Wang Rui Ye Xinwen Ou Chen Wang Ning Yu Cheng Huang Jie Zhao Chunhui Cai Furong Zhang Damiano Buratto Taimoor Khan Yan Qiao Yuejin Hua Ruhong Zhou Bing Tian |
| author_sort | Shang Dai |
| collection | DOAJ |
| description | Abstract Protocell fitness under extreme prebiotic conditions is critical in understanding the origin of life. However, little is known about protocell’s survival and fitness under prebiotic radiations. Here we present a radioresistant protocell model based on assembly of two types of coacervate droplets, which are formed through interactions of inorganic polyphosphate (polyP) with divalent metal cation and cationic tripeptide, respectively. Among the coacervate droplets, only the polyP-Mn droplet is radiotolerant and provides strong protection for recruited proteins. The radiosensitive polyP-tripeptide droplet sequestered with both proteins and DNA could be encapsulated inside the polyP-Mn droplet, and form into a compartmentalized protocell. The protocell protects the inner nucleoid-like condensate through efficient reactive oxygen species’ scavenging capacity of intracellular nonenzymic antioxidants including Mn-phosphate and Mn-peptide. Our results demonstrate a radioresistant protocell model with redox reaction system in response to ionizing radiation, which might enable the protocell fitness to prebiotic radiation on the primitive Earth preceding the emergence of enzyme-based fitness. This protocell might also provide applications in synthetic biology as bioreactor or drug delivery system. |
| first_indexed | 2024-03-09T01:17:29Z |
| format | Article |
| id | doaj.art-4fa5a975b14b448996ce81035d121c80 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-09T01:17:29Z |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-4fa5a975b14b448996ce81035d121c802023-12-10T12:23:28ZengNature PortfolioNature Communications2041-17232023-12-0114111410.1038/s41467-023-43272-5An inorganic mineral-based protocell with prebiotic radiation fitnessShang Dai0Zhenming Xie1Binqiang Wang2Rui Ye3Xinwen Ou4Chen Wang5Ning Yu6Cheng Huang7Jie Zhao8Chunhui Cai9Furong Zhang10Damiano Buratto11Taimoor Khan12Yan Qiao13Yuejin Hua14Ruhong Zhou15Bing Tian16Institute of Biophysics, College of Life Sciences, Zhejiang UniversityInstitute of Biophysics, College of Life Sciences, Zhejiang UniversityInstitute of Biophysics, College of Life Sciences, Zhejiang UniversitySchool of Physics, Institute of Quantitative Biology, Zhejiang UniversitySchool of Physics, Institute of Quantitative Biology, Zhejiang UniversityCollege of Pharmaceutical Science, Zhejiang UniversityInstitute of Biophysics, College of Life Sciences, Zhejiang UniversityInstitute of Biophysics, College of Life Sciences, Zhejiang UniversityInstitute of Biophysics, College of Life Sciences, Zhejiang UniversityInstitute of Biophysics, College of Life Sciences, Zhejiang UniversityInstitute of Biophysics, College of Life Sciences, Zhejiang UniversityInstitute of Biophysics, College of Life Sciences, Zhejiang UniversityInstitute of Biophysics, College of Life Sciences, Zhejiang UniversityInstitute of Chemistry, Chinese Academy of SciencesInstitute of Biophysics, College of Life Sciences, Zhejiang UniversityInstitute of Biophysics, College of Life Sciences, Zhejiang UniversityInstitute of Biophysics, College of Life Sciences, Zhejiang UniversityAbstract Protocell fitness under extreme prebiotic conditions is critical in understanding the origin of life. However, little is known about protocell’s survival and fitness under prebiotic radiations. Here we present a radioresistant protocell model based on assembly of two types of coacervate droplets, which are formed through interactions of inorganic polyphosphate (polyP) with divalent metal cation and cationic tripeptide, respectively. Among the coacervate droplets, only the polyP-Mn droplet is radiotolerant and provides strong protection for recruited proteins. The radiosensitive polyP-tripeptide droplet sequestered with both proteins and DNA could be encapsulated inside the polyP-Mn droplet, and form into a compartmentalized protocell. The protocell protects the inner nucleoid-like condensate through efficient reactive oxygen species’ scavenging capacity of intracellular nonenzymic antioxidants including Mn-phosphate and Mn-peptide. Our results demonstrate a radioresistant protocell model with redox reaction system in response to ionizing radiation, which might enable the protocell fitness to prebiotic radiation on the primitive Earth preceding the emergence of enzyme-based fitness. This protocell might also provide applications in synthetic biology as bioreactor or drug delivery system.https://doi.org/10.1038/s41467-023-43272-5 |
| spellingShingle | Shang Dai Zhenming Xie Binqiang Wang Rui Ye Xinwen Ou Chen Wang Ning Yu Cheng Huang Jie Zhao Chunhui Cai Furong Zhang Damiano Buratto Taimoor Khan Yan Qiao Yuejin Hua Ruhong Zhou Bing Tian An inorganic mineral-based protocell with prebiotic radiation fitness Nature Communications |
| title | An inorganic mineral-based protocell with prebiotic radiation fitness |
| title_full | An inorganic mineral-based protocell with prebiotic radiation fitness |
| title_fullStr | An inorganic mineral-based protocell with prebiotic radiation fitness |
| title_full_unstemmed | An inorganic mineral-based protocell with prebiotic radiation fitness |
| title_short | An inorganic mineral-based protocell with prebiotic radiation fitness |
| title_sort | inorganic mineral based protocell with prebiotic radiation fitness |
| url | https://doi.org/10.1038/s41467-023-43272-5 |
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