Ethanol extract separated from Sargassum horneri (Turner) abate LPS-induced inflammation in RAW 264.7 macrophages
Abstract Background This study is aimed at identifying the anti-inflammatory properties of 70% ethanol extract produced from an edible brown seaweed Sargassum horneri (SJB-SHE) with industrial-scale production by Seojin Biotech Co. Ltd. S. horneri is a rich source of nutrient and abundantly growing...
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| Format: | Article |
| Language: | English |
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The Korean Society of Fisheries and Aquatic Science
2019-02-01
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| Series: | Fisheries and Aquatic Sciences |
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| Online Access: | http://link.springer.com/article/10.1186/s41240-019-0121-8 |
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| author | K. K. Asanka Sanjeewa Thilina U. Jayawardena Hyun-Soo Kim Seo-Young Kim Ginnae Ahn Hak-Ju Kim Xiaoting Fu Youngheun Jee You-Jin Jeon |
| author_facet | K. K. Asanka Sanjeewa Thilina U. Jayawardena Hyun-Soo Kim Seo-Young Kim Ginnae Ahn Hak-Ju Kim Xiaoting Fu Youngheun Jee You-Jin Jeon |
| author_sort | K. K. Asanka Sanjeewa |
| collection | DOAJ |
| description | Abstract Background This study is aimed at identifying the anti-inflammatory properties of 70% ethanol extract produced from an edible brown seaweed Sargassum horneri (SJB-SHE) with industrial-scale production by Seojin Biotech Co. Ltd. S. horneri is a rich source of nutrient and abundantly growing along the shores of Jeju, South Korea. Methods Here, we investigated the effect of SJB-SHE on LPS-activated RAW 264.7 macrophages. The cytotoxicity and NO production of SJB-SHE were evaluated using MTT and Griess assays, respectively. Additionally, protein expression and gene expression levels were quantified using ELISA, Western blots, and RT-qPCR. Results Our results indicated that pre-treatment of RAW 264.7 macrophages with SJB-SHE significantly inhibited LPS-induced NO and PGE2 production. SJB-SHE downregulated the proteins and genes expression of LPS-induced iNOS and COX2. Additionally, SJB-SHE downregulated LPS-induced production of pro-inflammatory cytokines (tumor necrosis factor-α, interleukin (IL)-6, and IL-1β). Furthermore, SJB-SHE inhibited nuclear factor kappa-B (NF-κB) activation and translocation to the nucleus. SJB-SHE also suppressed the phosphorylation of mitogen-activated protein kinases (ERK1/2 and JNK). Conclusions Collectively, our results demonstrated that SJB-SHE has a potential anti-inflammatory property to use as a functional food ingredient in the future. |
| first_indexed | 2024-12-13T12:06:55Z |
| format | Article |
| id | doaj.art-4faa74c362da42e5b3586bb9ba60d008 |
| institution | Directory Open Access Journal |
| issn | 2234-1757 |
| language | English |
| last_indexed | 2024-12-13T12:06:55Z |
| publishDate | 2019-02-01 |
| publisher | The Korean Society of Fisheries and Aquatic Science |
| record_format | Article |
| series | Fisheries and Aquatic Sciences |
| spelling | doaj.art-4faa74c362da42e5b3586bb9ba60d0082022-12-21T23:46:55ZengThe Korean Society of Fisheries and Aquatic ScienceFisheries and Aquatic Sciences2234-17572019-02-0122111010.1186/s41240-019-0121-8Ethanol extract separated from Sargassum horneri (Turner) abate LPS-induced inflammation in RAW 264.7 macrophagesK. K. Asanka Sanjeewa0Thilina U. Jayawardena1Hyun-Soo Kim2Seo-Young Kim3Ginnae Ahn4Hak-Ju Kim5Xiaoting Fu6Youngheun Jee7You-Jin Jeon8Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National UniversityDepartment of Marine Life Science, School of Marine Biomedical Sciences, Jeju National UniversityDepartment of Marine Life Science, School of Marine Biomedical Sciences, Jeju National UniversityDepartment of Marine Life Science, School of Marine Biomedical Sciences, Jeju National UniversityDepartment of Food Technology and Nutrition, Chonnam National UniversitySeojin Biotech Co. Ltd, Acedongbaek TowerCollege of Food Science and Engineering, Ocean University of ChinaDepartment of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National UniversityDepartment of Marine Life Science, School of Marine Biomedical Sciences, Jeju National UniversityAbstract Background This study is aimed at identifying the anti-inflammatory properties of 70% ethanol extract produced from an edible brown seaweed Sargassum horneri (SJB-SHE) with industrial-scale production by Seojin Biotech Co. Ltd. S. horneri is a rich source of nutrient and abundantly growing along the shores of Jeju, South Korea. Methods Here, we investigated the effect of SJB-SHE on LPS-activated RAW 264.7 macrophages. The cytotoxicity and NO production of SJB-SHE were evaluated using MTT and Griess assays, respectively. Additionally, protein expression and gene expression levels were quantified using ELISA, Western blots, and RT-qPCR. Results Our results indicated that pre-treatment of RAW 264.7 macrophages with SJB-SHE significantly inhibited LPS-induced NO and PGE2 production. SJB-SHE downregulated the proteins and genes expression of LPS-induced iNOS and COX2. Additionally, SJB-SHE downregulated LPS-induced production of pro-inflammatory cytokines (tumor necrosis factor-α, interleukin (IL)-6, and IL-1β). Furthermore, SJB-SHE inhibited nuclear factor kappa-B (NF-κB) activation and translocation to the nucleus. SJB-SHE also suppressed the phosphorylation of mitogen-activated protein kinases (ERK1/2 and JNK). Conclusions Collectively, our results demonstrated that SJB-SHE has a potential anti-inflammatory property to use as a functional food ingredient in the future.http://link.springer.com/article/10.1186/s41240-019-0121-8Sargassum horneriEthanol extractRAW 264.7 macrophagesAnti-inflammationNF-κBMAPKs |
| spellingShingle | K. K. Asanka Sanjeewa Thilina U. Jayawardena Hyun-Soo Kim Seo-Young Kim Ginnae Ahn Hak-Ju Kim Xiaoting Fu Youngheun Jee You-Jin Jeon Ethanol extract separated from Sargassum horneri (Turner) abate LPS-induced inflammation in RAW 264.7 macrophages Fisheries and Aquatic Sciences Sargassum horneri Ethanol extract RAW 264.7 macrophages Anti-inflammation NF-κB MAPKs |
| title | Ethanol extract separated from Sargassum horneri (Turner) abate LPS-induced inflammation in RAW 264.7 macrophages |
| title_full | Ethanol extract separated from Sargassum horneri (Turner) abate LPS-induced inflammation in RAW 264.7 macrophages |
| title_fullStr | Ethanol extract separated from Sargassum horneri (Turner) abate LPS-induced inflammation in RAW 264.7 macrophages |
| title_full_unstemmed | Ethanol extract separated from Sargassum horneri (Turner) abate LPS-induced inflammation in RAW 264.7 macrophages |
| title_short | Ethanol extract separated from Sargassum horneri (Turner) abate LPS-induced inflammation in RAW 264.7 macrophages |
| title_sort | ethanol extract separated from sargassum horneri turner abate lps induced inflammation in raw 264 7 macrophages |
| topic | Sargassum horneri Ethanol extract RAW 264.7 macrophages Anti-inflammation NF-κB MAPKs |
| url | http://link.springer.com/article/10.1186/s41240-019-0121-8 |
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