Photon activated therapy (PAT) using monochromatic Synchrotron x-rays and iron oxide nanoparticles in a mouse tumor model: feasibility study of PAT for the treatment of superficial malignancy
<p>Abstract</p> <p>Background</p> <p>X-rays are known to interact with metallic nanoparticles, producing photoelectric species as radiosensitizing effects, and have been exploited <it>in vivo</it> mainly with gold nanoparticles. The purpose of this study was...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-10-01
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| Series: | Radiation Oncology |
| Subjects: | |
| Online Access: | http://www.ro-journal.com/content/7/1/184 |
| _version_ | 1819088438045966336 |
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| author | Choi Gi-Hwan Seo Seung-Jun Kim Ki-Hong Kim Hong-Tae Park Sung-Hwan Lim Jae-Hong Kim Jong-Ki |
| author_facet | Choi Gi-Hwan Seo Seung-Jun Kim Ki-Hong Kim Hong-Tae Park Sung-Hwan Lim Jae-Hong Kim Jong-Ki |
| author_sort | Choi Gi-Hwan |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>X-rays are known to interact with metallic nanoparticles, producing photoelectric species as radiosensitizing effects, and have been exploited <it>in vivo</it> mainly with gold nanoparticles. The purpose of this study was to investigate the potential of sensitizing effect of iron oxide nanoparticles for photon activated therapy.</p> <p>Methods</p> <p>X-rays photon activated therapy (PAT) was studied by treating CT26 tumor cells and CT26 tumor-bearing mice loaded with 13-nm diameter FeO NP, and irradiating them at 7.1 keV near the Fe K-edge using synchrotron x-rays radiation. Survival of cells was determined by MTT assay, and tumor regression assay was performed for in vivo model experiment. The results of PAT treated groups were compared with x-rays alone control groups.</p> <p>Results</p> <p>A more significant reduction in viability and damage was observed in the FeO NP-treated irradiated cells, compared to the radiation alone group (<it>p</it> < 0.04). Injection of FeO NP (100 mg/kg) 30 min prior to irradiation elevated the tumor concentration of magnetite to 40 μg of Fe/g tissue, with a tumor-to-muscle ratio of 17.4. The group receiving FeO NP and radiation of 10 Gy showed 80% complete tumor regression (CTR) after 15–35 days and relapse-free survival for up to 6 months, compared to the control group, which showed growth retardation, resulting in 80% fatality. The group receiving radiation of 40 Gy showed 100% CTR in all cases irrespective of the presence of FeO NP, but CTR was achieved earlier in the PAT-treated group compared with the radiation alone group.</p> <p>Conclusions</p> <p>An iron oxide nanoparticle enhanced therapeutic effect with relatively low tissue concentration of iron and 10 Gy of monochromatic X-rays. Since 7.1 keV X-rays is attenuated very sharply in the tissue, FeO NP-PAT may have promise as a potent treatment option for superficial malignancies in the skin, like chest wall recurrence of breast cancer.</p> |
| first_indexed | 2024-12-21T21:52:02Z |
| format | Article |
| id | doaj.art-4faa778704564ffea60d17e6d67c770f |
| institution | Directory Open Access Journal |
| issn | 1748-717X |
| language | English |
| last_indexed | 2024-12-21T21:52:02Z |
| publishDate | 2012-10-01 |
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| series | Radiation Oncology |
| spelling | doaj.art-4faa778704564ffea60d17e6d67c770f2022-12-21T18:49:04ZengBMCRadiation Oncology1748-717X2012-10-017118410.1186/1748-717X-7-184Photon activated therapy (PAT) using monochromatic Synchrotron x-rays and iron oxide nanoparticles in a mouse tumor model: feasibility study of PAT for the treatment of superficial malignancyChoi Gi-HwanSeo Seung-JunKim Ki-HongKim Hong-TaePark Sung-HwanLim Jae-HongKim Jong-Ki<p>Abstract</p> <p>Background</p> <p>X-rays are known to interact with metallic nanoparticles, producing photoelectric species as radiosensitizing effects, and have been exploited <it>in vivo</it> mainly with gold nanoparticles. The purpose of this study was to investigate the potential of sensitizing effect of iron oxide nanoparticles for photon activated therapy.</p> <p>Methods</p> <p>X-rays photon activated therapy (PAT) was studied by treating CT26 tumor cells and CT26 tumor-bearing mice loaded with 13-nm diameter FeO NP, and irradiating them at 7.1 keV near the Fe K-edge using synchrotron x-rays radiation. Survival of cells was determined by MTT assay, and tumor regression assay was performed for in vivo model experiment. The results of PAT treated groups were compared with x-rays alone control groups.</p> <p>Results</p> <p>A more significant reduction in viability and damage was observed in the FeO NP-treated irradiated cells, compared to the radiation alone group (<it>p</it> < 0.04). Injection of FeO NP (100 mg/kg) 30 min prior to irradiation elevated the tumor concentration of magnetite to 40 μg of Fe/g tissue, with a tumor-to-muscle ratio of 17.4. The group receiving FeO NP and radiation of 10 Gy showed 80% complete tumor regression (CTR) after 15–35 days and relapse-free survival for up to 6 months, compared to the control group, which showed growth retardation, resulting in 80% fatality. The group receiving radiation of 40 Gy showed 100% CTR in all cases irrespective of the presence of FeO NP, but CTR was achieved earlier in the PAT-treated group compared with the radiation alone group.</p> <p>Conclusions</p> <p>An iron oxide nanoparticle enhanced therapeutic effect with relatively low tissue concentration of iron and 10 Gy of monochromatic X-rays. Since 7.1 keV X-rays is attenuated very sharply in the tissue, FeO NP-PAT may have promise as a potent treatment option for superficial malignancies in the skin, like chest wall recurrence of breast cancer.</p>http://www.ro-journal.com/content/7/1/184Photon activated therapyIron oxide nanoparticlesAuger electronSynchrotron monochromatic x-raysSuperficial malignancy |
| spellingShingle | Choi Gi-Hwan Seo Seung-Jun Kim Ki-Hong Kim Hong-Tae Park Sung-Hwan Lim Jae-Hong Kim Jong-Ki Photon activated therapy (PAT) using monochromatic Synchrotron x-rays and iron oxide nanoparticles in a mouse tumor model: feasibility study of PAT for the treatment of superficial malignancy Radiation Oncology Photon activated therapy Iron oxide nanoparticles Auger electron Synchrotron monochromatic x-rays Superficial malignancy |
| title | Photon activated therapy (PAT) using monochromatic Synchrotron x-rays and iron oxide nanoparticles in a mouse tumor model: feasibility study of PAT for the treatment of superficial malignancy |
| title_full | Photon activated therapy (PAT) using monochromatic Synchrotron x-rays and iron oxide nanoparticles in a mouse tumor model: feasibility study of PAT for the treatment of superficial malignancy |
| title_fullStr | Photon activated therapy (PAT) using monochromatic Synchrotron x-rays and iron oxide nanoparticles in a mouse tumor model: feasibility study of PAT for the treatment of superficial malignancy |
| title_full_unstemmed | Photon activated therapy (PAT) using monochromatic Synchrotron x-rays and iron oxide nanoparticles in a mouse tumor model: feasibility study of PAT for the treatment of superficial malignancy |
| title_short | Photon activated therapy (PAT) using monochromatic Synchrotron x-rays and iron oxide nanoparticles in a mouse tumor model: feasibility study of PAT for the treatment of superficial malignancy |
| title_sort | photon activated therapy pat using monochromatic synchrotron x rays and iron oxide nanoparticles in a mouse tumor model feasibility study of pat for the treatment of superficial malignancy |
| topic | Photon activated therapy Iron oxide nanoparticles Auger electron Synchrotron monochromatic x-rays Superficial malignancy |
| url | http://www.ro-journal.com/content/7/1/184 |
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