Sonicated <i>Bordetella bronchiseptica</i> Bacterin Can Protect Dendritic Cells from Differential Cytotoxicity Caused by Doxorubicin and Vincristine and Enhance Their Antigen-Presenting Capability
Doxorubicin (DOX) and vincristine (VC) are anti-cancer drugs commonly used for lymphoma in veterinary and human medicine. However, there are several side effects caused by these drugs. In this study, the protective effects of sonicated <i>Bordetella bronchiseptica</i> bacterin (sBb) on d...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/44/7/213 |
| _version_ | 1797496775882309632 |
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| author | Ji Yun Sung Hong-Gu Joo |
| author_facet | Ji Yun Sung Hong-Gu Joo |
| author_sort | Ji Yun Sung |
| collection | DOAJ |
| description | Doxorubicin (DOX) and vincristine (VC) are anti-cancer drugs commonly used for lymphoma in veterinary and human medicine. However, there are several side effects caused by these drugs. In this study, the protective effects of sonicated <i>Bordetella bronchiseptica</i> bacterin (sBb) on dendritic cells (DCs) damaged by two anti-cancer drugs were investigated. DCs play important roles in the innate and adaptive immunity of hosts, especially activating T cells that can suppress tumor growth. The metabolic activity of DCs significantly increased after the treatment with sBb compared to that of control DCs. In addition, there was a marked change in mitochondrial integrity between DOX-treated DC and DOX + sBb-treated DCs. Flow cytometric analysis also demonstrated that sBb upregulated the expression of the surface markers of DCs, particularly CD54. In mixed lymphocyte responses, sBb significantly increased the antigen-presenting capability of DCs. In particular, sBb increased the capability of control DCs by approximately 150% and that of VC-treated DCs by 221%. These results suggest that sBb can be used as a potential immunostimulatory agent to protect DCs from anti-cancer drug-induced damage and provide fundamental information about using a combination of DCs and vincristine in immunotherapy. |
| first_indexed | 2024-03-10T03:08:27Z |
| format | Article |
| id | doaj.art-4fae685a9c8d4732984ba55690149d03 |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-10T03:08:27Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-4fae685a9c8d4732984ba55690149d032023-11-23T12:36:07ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452022-07-014473089309910.3390/cimb44070213Sonicated <i>Bordetella bronchiseptica</i> Bacterin Can Protect Dendritic Cells from Differential Cytotoxicity Caused by Doxorubicin and Vincristine and Enhance Their Antigen-Presenting CapabilityJi Yun Sung0Hong-Gu Joo1Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University, Jeju 63243, KoreaLaboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University, Jeju 63243, KoreaDoxorubicin (DOX) and vincristine (VC) are anti-cancer drugs commonly used for lymphoma in veterinary and human medicine. However, there are several side effects caused by these drugs. In this study, the protective effects of sonicated <i>Bordetella bronchiseptica</i> bacterin (sBb) on dendritic cells (DCs) damaged by two anti-cancer drugs were investigated. DCs play important roles in the innate and adaptive immunity of hosts, especially activating T cells that can suppress tumor growth. The metabolic activity of DCs significantly increased after the treatment with sBb compared to that of control DCs. In addition, there was a marked change in mitochondrial integrity between DOX-treated DC and DOX + sBb-treated DCs. Flow cytometric analysis also demonstrated that sBb upregulated the expression of the surface markers of DCs, particularly CD54. In mixed lymphocyte responses, sBb significantly increased the antigen-presenting capability of DCs. In particular, sBb increased the capability of control DCs by approximately 150% and that of VC-treated DCs by 221%. These results suggest that sBb can be used as a potential immunostimulatory agent to protect DCs from anti-cancer drug-induced damage and provide fundamental information about using a combination of DCs and vincristine in immunotherapy.https://www.mdpi.com/1467-3045/44/7/213doxorubicinvincristinedendritic cellsonicated <i>Bordetella bronchiseptica</i>protective effects |
| spellingShingle | Ji Yun Sung Hong-Gu Joo Sonicated <i>Bordetella bronchiseptica</i> Bacterin Can Protect Dendritic Cells from Differential Cytotoxicity Caused by Doxorubicin and Vincristine and Enhance Their Antigen-Presenting Capability Current Issues in Molecular Biology doxorubicin vincristine dendritic cell sonicated <i>Bordetella bronchiseptica</i> protective effects |
| title | Sonicated <i>Bordetella bronchiseptica</i> Bacterin Can Protect Dendritic Cells from Differential Cytotoxicity Caused by Doxorubicin and Vincristine and Enhance Their Antigen-Presenting Capability |
| title_full | Sonicated <i>Bordetella bronchiseptica</i> Bacterin Can Protect Dendritic Cells from Differential Cytotoxicity Caused by Doxorubicin and Vincristine and Enhance Their Antigen-Presenting Capability |
| title_fullStr | Sonicated <i>Bordetella bronchiseptica</i> Bacterin Can Protect Dendritic Cells from Differential Cytotoxicity Caused by Doxorubicin and Vincristine and Enhance Their Antigen-Presenting Capability |
| title_full_unstemmed | Sonicated <i>Bordetella bronchiseptica</i> Bacterin Can Protect Dendritic Cells from Differential Cytotoxicity Caused by Doxorubicin and Vincristine and Enhance Their Antigen-Presenting Capability |
| title_short | Sonicated <i>Bordetella bronchiseptica</i> Bacterin Can Protect Dendritic Cells from Differential Cytotoxicity Caused by Doxorubicin and Vincristine and Enhance Their Antigen-Presenting Capability |
| title_sort | sonicated i bordetella bronchiseptica i bacterin can protect dendritic cells from differential cytotoxicity caused by doxorubicin and vincristine and enhance their antigen presenting capability |
| topic | doxorubicin vincristine dendritic cell sonicated <i>Bordetella bronchiseptica</i> protective effects |
| url | https://www.mdpi.com/1467-3045/44/7/213 |
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