Characterization of the Cellular Reaction to a Collagen-Based Matrix: An In Vivo Histological and Histomorphometrical Analysis
The permeability and inflammatory tissue reaction to Mucomaix<sup>®</sup> matrix (MM), a non- cross-linked collagen-based matrix was evaluated in both ex vivo and in vivo settings. Liquid platelet rich fibrin (PRF), a blood concentrate system, was used to assess its capacity to absorb hu...
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| Online Access: | https://www.mdpi.com/1996-1944/13/12/2730 |
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| author | Samuel Ebele Udeabor Carlos Herrera-Vizcaíno Robert Sader C. James Kirkpatrick Sarah Al-Maawi Shahram Ghanaati |
| author_facet | Samuel Ebele Udeabor Carlos Herrera-Vizcaíno Robert Sader C. James Kirkpatrick Sarah Al-Maawi Shahram Ghanaati |
| author_sort | Samuel Ebele Udeabor |
| collection | DOAJ |
| description | The permeability and inflammatory tissue reaction to Mucomaix<sup>®</sup> matrix (MM), a non- cross-linked collagen-based matrix was evaluated in both ex vivo and in vivo settings. Liquid platelet rich fibrin (PRF), a blood concentrate system, was used to assess its capacity to absorb human proteins and interact with blood cells ex vivo. In the in vivo aspect, 12 Wister rats had MM implanted subcutaneously, whereas another 12 rats (control) were sham-operated without biomaterial implantation. On days 3, 15 and 30, explantation was completed (four rats per time-point) to evaluate the tissue reactions to the matrix. Data collected were statistically analyzed using analysis of variance (ANOVA) and Tukey multiple comparisons tests (GraphPad Prism 8). The matrix absorbed the liquid PRF in the ex vivo study. Day 3 post-implantation revealed mild tissue inflammatory reaction with presence of mononuclear cells in the implantation site and on the biomaterial surface (mostly CD68-positive macrophages). The control group at this stage had more mononuclear cells than the test group. From day 15, multinucleated giant cells (MNGCs) were seen in the implantation site and the outer third of the matrix with marked increase on day 30 and spread to the matrix core. The presence of these CD68-positive MNGCs was associated with significant matrix vascularization. The matrix degraded significantly over the study period, but its core was still visible as of day 30 post-implantation. The high permeability and fast degradation properties of MM were highlighted. |
| first_indexed | 2024-03-10T19:07:58Z |
| format | Article |
| id | doaj.art-4faebfe7c3f245b48d6e24212dc1ddd8 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T19:07:58Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-4faebfe7c3f245b48d6e24212dc1ddd82023-11-20T03:58:53ZengMDPI AGMaterials1996-19442020-06-011312273010.3390/ma13122730Characterization of the Cellular Reaction to a Collagen-Based Matrix: An In Vivo Histological and Histomorphometrical AnalysisSamuel Ebele Udeabor0Carlos Herrera-Vizcaíno1Robert Sader2C. James Kirkpatrick3Sarah Al-Maawi4Shahram Ghanaati5Department for Oral, Cranio-Maxillofacial, and Facial Plastic Surgery, Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Johann Wolfgang Goethe University, 60590 Frankfurt am Main, GermanyDepartment for Oral, Cranio-Maxillofacial, and Facial Plastic Surgery, Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Johann Wolfgang Goethe University, 60590 Frankfurt am Main, GermanyDepartment for Oral, Cranio-Maxillofacial, and Facial Plastic Surgery, Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Johann Wolfgang Goethe University, 60590 Frankfurt am Main, GermanyDepartment for Oral, Cranio-Maxillofacial, and Facial Plastic Surgery, Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Johann Wolfgang Goethe University, 60590 Frankfurt am Main, GermanyDepartment for Oral, Cranio-Maxillofacial, and Facial Plastic Surgery, Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Johann Wolfgang Goethe University, 60590 Frankfurt am Main, GermanyDepartment for Oral, Cranio-Maxillofacial, and Facial Plastic Surgery, Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Johann Wolfgang Goethe University, 60590 Frankfurt am Main, GermanyThe permeability and inflammatory tissue reaction to Mucomaix<sup>®</sup> matrix (MM), a non- cross-linked collagen-based matrix was evaluated in both ex vivo and in vivo settings. Liquid platelet rich fibrin (PRF), a blood concentrate system, was used to assess its capacity to absorb human proteins and interact with blood cells ex vivo. In the in vivo aspect, 12 Wister rats had MM implanted subcutaneously, whereas another 12 rats (control) were sham-operated without biomaterial implantation. On days 3, 15 and 30, explantation was completed (four rats per time-point) to evaluate the tissue reactions to the matrix. Data collected were statistically analyzed using analysis of variance (ANOVA) and Tukey multiple comparisons tests (GraphPad Prism 8). The matrix absorbed the liquid PRF in the ex vivo study. Day 3 post-implantation revealed mild tissue inflammatory reaction with presence of mononuclear cells in the implantation site and on the biomaterial surface (mostly CD68-positive macrophages). The control group at this stage had more mononuclear cells than the test group. From day 15, multinucleated giant cells (MNGCs) were seen in the implantation site and the outer third of the matrix with marked increase on day 30 and spread to the matrix core. The presence of these CD68-positive MNGCs was associated with significant matrix vascularization. The matrix degraded significantly over the study period, but its core was still visible as of day 30 post-implantation. The high permeability and fast degradation properties of MM were highlighted.https://www.mdpi.com/1996-1944/13/12/2730cellular reactioncollagen-based matrixMucomaix<sup>®</sup> matrixhistologyhistomorphometryliquid PRF |
| spellingShingle | Samuel Ebele Udeabor Carlos Herrera-Vizcaíno Robert Sader C. James Kirkpatrick Sarah Al-Maawi Shahram Ghanaati Characterization of the Cellular Reaction to a Collagen-Based Matrix: An In Vivo Histological and Histomorphometrical Analysis Materials cellular reaction collagen-based matrix Mucomaix<sup>®</sup> matrix histology histomorphometry liquid PRF |
| title | Characterization of the Cellular Reaction to a Collagen-Based Matrix: An In Vivo Histological and Histomorphometrical Analysis |
| title_full | Characterization of the Cellular Reaction to a Collagen-Based Matrix: An In Vivo Histological and Histomorphometrical Analysis |
| title_fullStr | Characterization of the Cellular Reaction to a Collagen-Based Matrix: An In Vivo Histological and Histomorphometrical Analysis |
| title_full_unstemmed | Characterization of the Cellular Reaction to a Collagen-Based Matrix: An In Vivo Histological and Histomorphometrical Analysis |
| title_short | Characterization of the Cellular Reaction to a Collagen-Based Matrix: An In Vivo Histological and Histomorphometrical Analysis |
| title_sort | characterization of the cellular reaction to a collagen based matrix an in vivo histological and histomorphometrical analysis |
| topic | cellular reaction collagen-based matrix Mucomaix<sup>®</sup> matrix histology histomorphometry liquid PRF |
| url | https://www.mdpi.com/1996-1944/13/12/2730 |
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