Clusters of Drug-Resistant Mycobacterium tuberculosis Detected by Whole-Genome Sequence Analysis of Nationwide Sample, Thailand, 2014–2017
Multidrug-resistant tuberculosis (MDR TB), pre-extensively drug-resistant tuberculosis (pre-XDR TB), and extensively drug-resistant tuberculosis (XDR TB) complicate disease control. We analyzed whole-genome sequence data for 579 phenotypically drug-resistant M. tuberculosis isolates (28% of availabl...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2021-03-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/27/3/20-4364_article |
| _version_ | 1828929685642280960 |
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| author | Ditthawat Nonghanphithak Angkana Chaiprasert Saijai Smithtikarn Phalin Kamolwat Petchawan Pungrassami Virasakdi Chongsuvivatwong Surakameth Mahasirimongkol Wipa Reechaipichitkul Chaniya Leepiyasakulchai Jody E. Phelan David Blair Taane G. Clark Kiatichai Faksri |
| author_facet | Ditthawat Nonghanphithak Angkana Chaiprasert Saijai Smithtikarn Phalin Kamolwat Petchawan Pungrassami Virasakdi Chongsuvivatwong Surakameth Mahasirimongkol Wipa Reechaipichitkul Chaniya Leepiyasakulchai Jody E. Phelan David Blair Taane G. Clark Kiatichai Faksri |
| author_sort | Ditthawat Nonghanphithak |
| collection | DOAJ |
| description | Multidrug-resistant tuberculosis (MDR TB), pre-extensively drug-resistant tuberculosis (pre-XDR TB), and extensively drug-resistant tuberculosis (XDR TB) complicate disease control. We analyzed whole-genome sequence data for 579 phenotypically drug-resistant M. tuberculosis isolates (28% of available MDR/pre-XDR and all culturable XDR TB isolates collected in Thailand during 2014–2017). Most isolates were from lineage 2 (n = 482; 83.2%). Cluster analysis revealed that 281/579 isolates (48.5%) formed 89 clusters, including 205 MDR TB, 46 pre-XDR TB, 19 XDR TB, and 11 poly–drug-resistant TB isolates based on genotypic drug resistance. Members of most clusters had the same subset of drug resistance-associated mutations, supporting potential primary resistance in MDR TB (n = 176/205; 85.9%), pre-XDR TB (n = 29/46; 63.0%), and XDR TB (n = 14/19; 73.7%). Thirteen major clades were significantly associated with geography (p<0.001). Clusters of clonal origin contribute greatly to the high prevalence of drug-resistant TB in Thailand. |
| first_indexed | 2024-12-14T00:22:49Z |
| format | Article |
| id | doaj.art-4fb168c6b80d440fbf927d4b1fad917d |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-14T00:22:49Z |
| publishDate | 2021-03-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-4fb168c6b80d440fbf927d4b1fad917d2022-12-21T23:25:09ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592021-03-0127381382210.3201/eid2703.204364Clusters of Drug-Resistant Mycobacterium tuberculosis Detected by Whole-Genome Sequence Analysis of Nationwide Sample, Thailand, 2014–2017Ditthawat NonghanphithakAngkana ChaiprasertSaijai SmithtikarnPhalin KamolwatPetchawan PungrassamiVirasakdi ChongsuvivatwongSurakameth MahasirimongkolWipa ReechaipichitkulChaniya LeepiyasakulchaiJody E. PhelanDavid BlairTaane G. ClarkKiatichai FaksriMultidrug-resistant tuberculosis (MDR TB), pre-extensively drug-resistant tuberculosis (pre-XDR TB), and extensively drug-resistant tuberculosis (XDR TB) complicate disease control. We analyzed whole-genome sequence data for 579 phenotypically drug-resistant M. tuberculosis isolates (28% of available MDR/pre-XDR and all culturable XDR TB isolates collected in Thailand during 2014–2017). Most isolates were from lineage 2 (n = 482; 83.2%). Cluster analysis revealed that 281/579 isolates (48.5%) formed 89 clusters, including 205 MDR TB, 46 pre-XDR TB, 19 XDR TB, and 11 poly–drug-resistant TB isolates based on genotypic drug resistance. Members of most clusters had the same subset of drug resistance-associated mutations, supporting potential primary resistance in MDR TB (n = 176/205; 85.9%), pre-XDR TB (n = 29/46; 63.0%), and XDR TB (n = 14/19; 73.7%). Thirteen major clades were significantly associated with geography (p<0.001). Clusters of clonal origin contribute greatly to the high prevalence of drug-resistant TB in Thailand.https://wwwnc.cdc.gov/eid/article/27/3/20-4364_articleMycobacterium tuberculosisdrug resistancemultidrug-resistant TBtransmissionwhole-genome sequencingThailand |
| spellingShingle | Ditthawat Nonghanphithak Angkana Chaiprasert Saijai Smithtikarn Phalin Kamolwat Petchawan Pungrassami Virasakdi Chongsuvivatwong Surakameth Mahasirimongkol Wipa Reechaipichitkul Chaniya Leepiyasakulchai Jody E. Phelan David Blair Taane G. Clark Kiatichai Faksri Clusters of Drug-Resistant Mycobacterium tuberculosis Detected by Whole-Genome Sequence Analysis of Nationwide Sample, Thailand, 2014–2017 Emerging Infectious Diseases Mycobacterium tuberculosis drug resistance multidrug-resistant TB transmission whole-genome sequencing Thailand |
| title | Clusters of Drug-Resistant Mycobacterium tuberculosis Detected by Whole-Genome Sequence Analysis of Nationwide Sample, Thailand, 2014–2017 |
| title_full | Clusters of Drug-Resistant Mycobacterium tuberculosis Detected by Whole-Genome Sequence Analysis of Nationwide Sample, Thailand, 2014–2017 |
| title_fullStr | Clusters of Drug-Resistant Mycobacterium tuberculosis Detected by Whole-Genome Sequence Analysis of Nationwide Sample, Thailand, 2014–2017 |
| title_full_unstemmed | Clusters of Drug-Resistant Mycobacterium tuberculosis Detected by Whole-Genome Sequence Analysis of Nationwide Sample, Thailand, 2014–2017 |
| title_short | Clusters of Drug-Resistant Mycobacterium tuberculosis Detected by Whole-Genome Sequence Analysis of Nationwide Sample, Thailand, 2014–2017 |
| title_sort | clusters of drug resistant mycobacterium tuberculosis detected by whole genome sequence analysis of nationwide sample thailand 2014 2017 |
| topic | Mycobacterium tuberculosis drug resistance multidrug-resistant TB transmission whole-genome sequencing Thailand |
| url | https://wwwnc.cdc.gov/eid/article/27/3/20-4364_article |
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