A combination of Schwann-cell grafts and aerobic exercise enhances sciatic nerve regeneration.
Despite the regenerative potential of the peripheral nervous system, severe nerve lesions lead to loss of target-organ innervation, making complete functional recovery a challenge. Few studies have given attention to combining different approaches in order to accelerate the regenerative process.Test...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4198198?pdf=render |
| _version_ | 1818026843075248128 |
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| author | Camila Oliveira Goulart Sofia Jürgensen Allana Souto Júlia Teixeira Oliveira Silmara de Lima Chiara Tonda-Turo Suelen Adriani Marques Fernanda Martins de Almeida Ana Maria Blanco Martinez |
| author_facet | Camila Oliveira Goulart Sofia Jürgensen Allana Souto Júlia Teixeira Oliveira Silmara de Lima Chiara Tonda-Turo Suelen Adriani Marques Fernanda Martins de Almeida Ana Maria Blanco Martinez |
| author_sort | Camila Oliveira Goulart |
| collection | DOAJ |
| description | Despite the regenerative potential of the peripheral nervous system, severe nerve lesions lead to loss of target-organ innervation, making complete functional recovery a challenge. Few studies have given attention to combining different approaches in order to accelerate the regenerative process.Test the effectiveness of combining Schwann-cells transplantation into a biodegradable conduit, with treadmill training as a therapeutic strategy to improve the outcome of repair after mouse nerve injury.Sciatic nerve transection was performed in adult C57BL/6 mice; the proximal and distal stumps of the nerve were sutured into the conduit. Four groups were analyzed: acellular grafts (DMEM group), Schwann cell grafts (3×105/2 µL; SC group), treadmill training (TMT group), and treadmill training and Schwann cell grafts (TMT + SC group). Locomotor function was assessed weekly by Sciatic Function Index and Global Mobility Test. Animals were anesthetized after eight weeks and dissected for morphological analysis.Combined therapies improved nerve regeneration, and increased the number of myelinated fibers and myelin area compared to the DMEM group. Motor recovery was accelerated in the TMT + SC group, which showed significantly better values in sciatic function index and in global mobility test than in the other groups. The TMT + SC group showed increased levels of trophic-factor expression compared to DMEM, contributing to the better functional outcome observed in the former group. The number of neurons in L4 segments was significantly higher in the SC and TMT + SC groups when compared to DMEM group. Counts of dorsal root ganglion sensory neurons revealed that TMT group had a significant increased number of neurons compared to DMEM group, while the SC and TMT + SC groups had a slight but not significant increase in the total number of motor neurons.These data provide evidence that this combination of therapeutic strategies can significantly improve functional and morphological recovery after sciatic injury. |
| first_indexed | 2024-12-10T04:38:26Z |
| format | Article |
| id | doaj.art-4fb39da7bb56423faaff6a72fb523499 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T04:38:26Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4fb39da7bb56423faaff6a72fb5234992022-12-22T02:01:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e11009010.1371/journal.pone.0110090A combination of Schwann-cell grafts and aerobic exercise enhances sciatic nerve regeneration.Camila Oliveira GoulartSofia JürgensenAllana SoutoJúlia Teixeira OliveiraSilmara de LimaChiara Tonda-TuroSuelen Adriani MarquesFernanda Martins de AlmeidaAna Maria Blanco MartinezDespite the regenerative potential of the peripheral nervous system, severe nerve lesions lead to loss of target-organ innervation, making complete functional recovery a challenge. Few studies have given attention to combining different approaches in order to accelerate the regenerative process.Test the effectiveness of combining Schwann-cells transplantation into a biodegradable conduit, with treadmill training as a therapeutic strategy to improve the outcome of repair after mouse nerve injury.Sciatic nerve transection was performed in adult C57BL/6 mice; the proximal and distal stumps of the nerve were sutured into the conduit. Four groups were analyzed: acellular grafts (DMEM group), Schwann cell grafts (3×105/2 µL; SC group), treadmill training (TMT group), and treadmill training and Schwann cell grafts (TMT + SC group). Locomotor function was assessed weekly by Sciatic Function Index and Global Mobility Test. Animals were anesthetized after eight weeks and dissected for morphological analysis.Combined therapies improved nerve regeneration, and increased the number of myelinated fibers and myelin area compared to the DMEM group. Motor recovery was accelerated in the TMT + SC group, which showed significantly better values in sciatic function index and in global mobility test than in the other groups. The TMT + SC group showed increased levels of trophic-factor expression compared to DMEM, contributing to the better functional outcome observed in the former group. The number of neurons in L4 segments was significantly higher in the SC and TMT + SC groups when compared to DMEM group. Counts of dorsal root ganglion sensory neurons revealed that TMT group had a significant increased number of neurons compared to DMEM group, while the SC and TMT + SC groups had a slight but not significant increase in the total number of motor neurons.These data provide evidence that this combination of therapeutic strategies can significantly improve functional and morphological recovery after sciatic injury.http://europepmc.org/articles/PMC4198198?pdf=render |
| spellingShingle | Camila Oliveira Goulart Sofia Jürgensen Allana Souto Júlia Teixeira Oliveira Silmara de Lima Chiara Tonda-Turo Suelen Adriani Marques Fernanda Martins de Almeida Ana Maria Blanco Martinez A combination of Schwann-cell grafts and aerobic exercise enhances sciatic nerve regeneration. PLoS ONE |
| title | A combination of Schwann-cell grafts and aerobic exercise enhances sciatic nerve regeneration. |
| title_full | A combination of Schwann-cell grafts and aerobic exercise enhances sciatic nerve regeneration. |
| title_fullStr | A combination of Schwann-cell grafts and aerobic exercise enhances sciatic nerve regeneration. |
| title_full_unstemmed | A combination of Schwann-cell grafts and aerobic exercise enhances sciatic nerve regeneration. |
| title_short | A combination of Schwann-cell grafts and aerobic exercise enhances sciatic nerve regeneration. |
| title_sort | combination of schwann cell grafts and aerobic exercise enhances sciatic nerve regeneration |
| url | http://europepmc.org/articles/PMC4198198?pdf=render |
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