2D DIGE proteomic analysis highlights delayed postnatal repression of α‐fetoprotein expression in homocystinuria model mice
Cystathionine β‐synthase‐deficient (Cbs−/−) mice, an animal model for homocystinuria, exhibit hepatic steatosis and juvenile semilethality via as yet unknown mechanisms. The plasma protein profile ofCbs−/− mice was investigated by proteomic analysis using two‐dimensional difference gel electrophores...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1016/j.fob.2015.06.008 |
| _version_ | 1811185075487768576 |
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| author | Shotaro Kamata Noriyuki Akahoshi Isao Ishii |
| author_facet | Shotaro Kamata Noriyuki Akahoshi Isao Ishii |
| author_sort | Shotaro Kamata |
| collection | DOAJ |
| description | Cystathionine β‐synthase‐deficient (Cbs−/−) mice, an animal model for homocystinuria, exhibit hepatic steatosis and juvenile semilethality via as yet unknown mechanisms. The plasma protein profile ofCbs−/− mice was investigated by proteomic analysis using two‐dimensional difference gel electrophoresis and matrix‐assisted laser desorption/ionization‐time of flight/mass spectrometry. We found hyperaccumulation of α‐fetoprotein (AFP) and downregulation of most other plasma proteins. AFP was highly expressed in fetal liver, but its expression declined dramatically via transcriptional repression after birth in both wild‐type andCbs−/− mice. However, the repression was delayed inCbs−/− mice, causing high postnatal AFP levels, which may relate to transcriptional repression of most plasma proteins originating from liver and the observed hepatic dysfunction. |
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| format | Article |
| id | doaj.art-4fb54efd06fa427cbfa40306e152f7d4 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-11T13:24:34Z |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-4fb54efd06fa427cbfa40306e152f7d42022-12-22T04:22:07ZengWileyFEBS Open Bio2211-54632015-01-015153554110.1016/j.fob.2015.06.0082D DIGE proteomic analysis highlights delayed postnatal repression of α‐fetoprotein expression in homocystinuria model miceShotaro Kamata0Noriyuki Akahoshi1Isao Ishii2Department of Biochemistry, Keio University Graduate School of Pharmaceutical Sciences, Tokyo 105-8512, JapanDepartment of Immunology, Akita University Graduate School of Medicine, Akita 010-8543, JapanDepartment of Biochemistry, Keio University Graduate School of Pharmaceutical Sciences, Tokyo 105-8512, JapanCystathionine β‐synthase‐deficient (Cbs−/−) mice, an animal model for homocystinuria, exhibit hepatic steatosis and juvenile semilethality via as yet unknown mechanisms. The plasma protein profile ofCbs−/− mice was investigated by proteomic analysis using two‐dimensional difference gel electrophoresis and matrix‐assisted laser desorption/ionization‐time of flight/mass spectrometry. We found hyperaccumulation of α‐fetoprotein (AFP) and downregulation of most other plasma proteins. AFP was highly expressed in fetal liver, but its expression declined dramatically via transcriptional repression after birth in both wild‐type andCbs−/− mice. However, the repression was delayed inCbs−/− mice, causing high postnatal AFP levels, which may relate to transcriptional repression of most plasma proteins originating from liver and the observed hepatic dysfunction.https://doi.org/10.1016/j.fob.2015.06.008α-FetoproteinCystathionine β-synthaseMALDI-TOF/MSProteomicsTranscriptional regulation2D DIGE |
| spellingShingle | Shotaro Kamata Noriyuki Akahoshi Isao Ishii 2D DIGE proteomic analysis highlights delayed postnatal repression of α‐fetoprotein expression in homocystinuria model mice FEBS Open Bio α-Fetoprotein Cystathionine β-synthase MALDI-TOF/MS Proteomics Transcriptional regulation 2D DIGE |
| title | 2D DIGE proteomic analysis highlights delayed postnatal repression of α‐fetoprotein expression in homocystinuria model mice |
| title_full | 2D DIGE proteomic analysis highlights delayed postnatal repression of α‐fetoprotein expression in homocystinuria model mice |
| title_fullStr | 2D DIGE proteomic analysis highlights delayed postnatal repression of α‐fetoprotein expression in homocystinuria model mice |
| title_full_unstemmed | 2D DIGE proteomic analysis highlights delayed postnatal repression of α‐fetoprotein expression in homocystinuria model mice |
| title_short | 2D DIGE proteomic analysis highlights delayed postnatal repression of α‐fetoprotein expression in homocystinuria model mice |
| title_sort | 2d dige proteomic analysis highlights delayed postnatal repression of α fetoprotein expression in homocystinuria model mice |
| topic | α-Fetoprotein Cystathionine β-synthase MALDI-TOF/MS Proteomics Transcriptional regulation 2D DIGE |
| url | https://doi.org/10.1016/j.fob.2015.06.008 |
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